Intragenic suppressors reveal long distance interactions between inactivating and reactivating amino acid replacements generating three-dimensional constraints in the structure of mitochondrial cytochrome b.

The identification of fish species in food products is problematic because morphological features of the fish are partially or completely lost during processing. It is important to determine fish origin because of the increasing international seafood trade and because European Community Regulation 104/2000 requires that the products be labeled correctly. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. This method allowed the identification of fish species in all samples. Fish in all of the examined products belonged to these two families, with the exception of one sample of smoked baccalà (salt cod), which was not included in the Gadidae cluster.

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The Importance of Ice Ages in Diversification of Arctic Collared Lemmings (Dicrostonyx): Evidence from the Mitochondrial Cytochrome B Region

Hereditas ◽

10.1111/j.1601-5223.1999.00301.x ◽

2004 ◽

Vol 130 (3) ◽

pp. 301-307 ◽

Cited By ~ 35

Author(s):

Vadim B. Fedorov ◽

Anna V. Goropashnaya

Keyword(s):

Cytochrome B ◽

Mitochondrial Cytochrome ◽

Ice Ages ◽

Mitochondrial Cytochrome B

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Genetic characterization and phylogenetic study of Indonesian indigenous catfish based on mitochondrial cytochrome B gene

Veterinary World ◽

10.14202/vetworld.2020.96-103 ◽

2020 ◽

Vol 13 (1) ◽

pp. 96-103

Author(s):

Dorothea Vera Megarani ◽

Herjuno Ari Nugroho ◽

Zahrah Prawita Andarini ◽

Yura Dwi Risa B. R. Surbakti ◽

Rini Widayanti

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Cytochrome B ◽

Genetic Characterization ◽

Phylogenetic Study ◽

Cyt B ◽

Phylogenetic Structure ◽

Mitochondrial Cytochrome B ◽

Sperata Seenghala ◽

Cyt B Gene

Aim: This study aimed to determine the genetic characterization and phylogenetic structure of Indonesian indigenous catfish using cytochrome B (Cyt B) sequences. Materials and Methods: The genomes of 26 catfishes caught from nine rivers from nine different geographical locations around Indonesia were analyzed. The tissue isolation method was used to isolate the total genome of the fishes. Furthermore, polymerase chain reaction was done to amplify the mtDNA Cyt B using the CytBF and CytBR primers. Following sequencing, the analysis of genetic variation and the phylogenetic relationship was performed using MEGA version X software. Results: Cyt B gene sequencing attained a total of 1139 nucleotides encrypting 379 amino acids for all samples. The ClustalW alignment program using MEGA X software revealed 395 substituted nucleotides, which then translated into 63 amino acid variation sites among all 26 samples. No amino acids in catfish BB were different compared to catfish PM, MP, and KR2,3. Catfish MS had one modified amino acid; KR1 and KS had two different amino acids; BF had 38 different amino acids; EM had 31 different amino acids; and BSBJ had 26 different amino acids compared to catfish BB. The most significant alteration of amino acids was between catfish EM and BF (49 amino acids). Conclusion: Indonesian catfish were divided into five clades based on the Cyt B gene. Samples KR and MP (Sumatra); MS and BB (Kalimantan); and PM (Java) were clustered with Hemibagrus nemurus and Hemibagrus wyckioides (Bagridae family). Samples from Kalimantan (KS) and one sample of KR (KR1) from Sumatra were clustered with Sperata seenghala and Hemibagrus spilopterus (Bagridae family). Samples from Java (BSBJ) were clustered with Pseudolais pleurotaenia (Pangasiidae family). Samples EM (Java) were together with Mystus cavasius (Bagridae family). Samples from West Papua were clustered with Potamosilurus latirostris (Ariidae family).

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Kajian Penanda Genetik Gen Sitokrom b DNA Mitokondria Ikan Lais dari Sungai Kampar Riau

Jurnal Natur Indonesia ◽

10.31258/jnat.10.1.6-12 ◽

2018 ◽

Vol 10 (1) ◽

pp. 6

Author(s):

Roza Elvyra ◽

Dedy Duryadi Solihin

Keyword(s):

Species Diversity ◽

Molecular Marker ◽

Phylogenetic Relationship ◽

Cytochrome B ◽

Mitochondrial Cytochrome ◽

Cyt B ◽

Phylogenetic Marker ◽

Mitochondrial Cytochrome B ◽

Cyt B Gene

The mitochondrial cytochrome b (cyt-b) gene as a phylogenetic marker of lais fish Kryptopterus schilbeides from Kampar River in Riau has been studied. This is a prelimininary research on the utility of cyt-b gene as a molecular marker to obtain species diversity and phylogenetic relationship among Kryptopterus fishes from Kampar River. The primers of L14841 and H15149 were used to amplify the cyt-b gene. The results showed that K. schilbeides has isoleusine at site-81 and metionine at site-114; K. schilbeides from Kampar River and K. schilbeides from GenBank form a phylogeny cluster at 45% value.

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