Mitochondrial Cytochrome b DNA Sequence Variations: An Approach to Fish Species Identification in Processed Fish Products

2005 ◽  
Vol 68 (2) ◽  
pp. 421-425 ◽  
Author(s):  
TIZIANA PEPE ◽  
MICHELE TROTTA ◽  
ISOLINA DI MARCO ◽  
PAOLA CENNAMO ◽  
ANIELLO ANASTASIO ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Cytochrome B ◽  
Fish Species ◽  
Mitochondrial Cytochrome ◽  
Fish Products ◽  
Community Regulation ◽  
Sequence Variations ◽  
Fish Species Identification ◽  
Mitochondrial Cytochrome B ◽  
Pcr Products

The identification of fish species in food products is problematic because morphological features of the fish are partially or completely lost during processing. It is important to determine fish origin because of the increasing international seafood trade and because European Community Regulation 104/2000 requires that the products be labeled correctly. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. This method allowed the identification of fish species in all samples. Fish in all of the examined products belonged to these two families, with the exception of one sample of smoked baccalà (salt cod), which was not included in the Gadidae cluster.

scholarly journals Wild Boar-Specific PCR Assay and Sequence Analysis Based on Mitochondrial Cytochrome-B Gene for Halal Authentication Studies

2020 ◽  
Vol 20 (2) ◽  
pp. 483
Author(s):  
Ganea Qorry Aina ◽  
Abdul Rohman ◽  
Yuny Erwanto
Keyword(s):  
Sequence Analysis ◽  
Wild Boar ◽  
Cytochrome B ◽  
Coefficient Of Determination ◽  
Mitochondrial Cytochrome ◽  
Cyt B ◽  
Mt Dna ◽  
Mitochondrial Cytochrome B ◽  
Amplicon Size ◽  
Halal Authentication

Wild boar meat (WBM) is non-halal meat widely abused in Indonesia. The most common case is mixing beef with WBM either in raw or processed foods. Therefore, it is necessary to develop a detection method of WBM contamination. The objective of this study was to employ polymerase chain reaction (PCR) and sequence analysis using species specific primer (SSP) targeting on wild boar mitochondrial cytochrome-b (CYTBWB2-wb) gene for the identification of WBM in a meatball. The specificity of primer was tested, and the amplicon size was confirmed with conventional PCR and agarose electrophoresis. The base sequences were analyzed using GeneStudio software and subjected to BLAST using NCBI. CYTBWB2-wb primer was also used to test the reference meatballs made from beef and WBM using real-time PCR. The result showed that CYTBWB2-wb amplified wild boar Cyt-B mt-DNA gene specifically. The amplicon size was 194 base pair (bp) with a similarity of 93–98% toward gen Cyt-B mt-DNA of several wild boar types. The primer is able to detect WBM on the reference meatballs up to 0.1% wt/wt with efficiency value of 108.0% and coefficient of determination (R2) of 0.970. The CYTBWB2-wb primer proved to be specific and could be used as a standard method to identify the presence of WBM contamination in meatball products for halal authentication studies.

scholarly journals First PCR isolation of Crithidia mellificae (Euglenozoa: Trypanosomatidae) in Apis mellifera (Hymenoptera: Apidae) in Italy

2015 ◽  
Vol 47 (1) ◽  
pp. 45 ◽  
Author(s):  
Antonella Cersini ◽  
Valeria Antognetti ◽  
Raffaella Conti ◽  
Fabiana Velletrani ◽  
Giovanni Formato
Keyword(s):  
Apis Mellifera ◽  
Cytochrome B ◽  
Ribosomal Rna ◽  
Short Communication ◽  
Small Subunit ◽  
Mitochondrial Cytochrome ◽  
Small Subunit Ribosomal Rna ◽  
Mitochondrial Cytochrome B ◽  
Pcr Products

<em>Crithidia mellificae</em> (Langridge &amp; McGhee, 1967) is a trypanosomatid described in <em>Apis</em> <em>mellifera</em> (Linnaeus, 1758). The pathological effects of this parasite on the host are not well known. In this short communication, we report the first isolation of this pathogen in Italy, as realized in December 2013. The detection of <em>Crithidia</em> spp. was obtained by applying two PCR protocols that target the sequence of the mitochondrial cytochrome b (<em>Cytb</em>) and the sequence of small subunit ribosomal RNA (<em>SSUrRNA</em>), respectively. The PCR products were subjected to sequencing, which confirmed that the strain belonged to <em>Crithidia mellificae</em>.

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