Optimization of Bacterial Concentrate Media for Lactobacillus Sakei

Two strains of lactic acid bacteria were selected for the study (Lactobacillus sakei Lsk-45andLactobacillus sakeiDSM 20017). Bacterial media, based on rice and rice flour, were tested as an alternative to media based on whey. A comparison of the different types of media showed that there was better growth of the selected strains on themedium based on rice flour.Statistical analyses, including factorial experiments and response surface analyses, were used to optimizethe composition of the bacterial medium for Lactobacillus sakei propagation. Bacteriological peptone and rice flour were found to be good growth factors for Lactobacillus sakei.For Lactobacillus sakei Lsk-45,better growth was obtained with the use of 7.75-10 g/L of peptone and 57.5-75 g/L of rice flour. For Lactobacillus sakei DSM 20017, better growth was obtained with the use of 7-10 g/L of peptone and 40-75 g/L of rice flour. Keywords: starters, media, Lactobacillus sakei, fermentation,fish products, bacterial strains, lactic acid bacteria

Research Paper DNA barcode identification of fish products from Guiyang markets in southwest China

Nowadays, the global fish consumption continues to rise along with the continuous growth of the population, which has led to the dilemma of overfishing of fishery resources. Especially high-value fish that are overfished are often replaced by other fish. Therefore, the accurate identification of fish products in the market is a problem worthy of attention. In this study, full-DNA barcoding (FDB) and mini-DNA barcoding (MDB) used to detect the fraud of fish products in Guiyang, Guizhou province in China. The molecular identification results showed that 39 of the 191 samples were not consistent with the labels. The mislabelling of fish products for fresh, frozen, cooked and canned were 11.70%, 20.00%, 34.09% and 50.00%, respectively. The average kimura 2 parameter distances of MDB within species and genera were 0.27% and 5.41%, respectively; while average distances of FDB were 0.17% within species and 6.17% within genera. In this study, commercial fraud is noticeable, most of the high-priced fish were replaced of low-priced fish with a similar feature. Our study indicated that DNA barcoding is a valid tool for the identification of fish products and that it allows an idea of conservation and monitoring efforts, while confirming the MDB as a reliable tool for fish products.

Isolation and Identification of major bacteria from three Ethiopian rift valley lakes live and processed fish, and water samples: Implications in sanitary system of fish products

Bacterial pathogens are a great threat to fish production. Gram-negative bacteria are among the major bacterial fish pathogens and zoonotic with the potential to infect humans. This cross-sectional study was conducted to isolate and identify major gram-negative bacteria from live and processed fish, and water samples from Lakes Hawassa, Langanoo and Ziway. A total of 674 different types of samples: 630 tissue samples (210 samples for each intestine, Kidney and liver collected from 210 live fish (Oreochromis niloticus, Cyprinus carpio and Clarias gariepinus), 20 processed fish samples from lake Ziway fish processing center and 24 lake water samples were included in the study from each lake. The mean values of pH, temperature, dissolved oxygen and nitrate in all water samples were within the normal range at which most freshwater fish species become non-stressed. Of a total of 674 samples included in the study, the bacteria were isolated from 154 (22.8%) samples with significant difference (P<0.05) observed in some isolates with respect to sample origin. Of these 154 isolates, 103(66.8%) isolates were gram-negative bacteria consisting of 15 species based on morphology and a range of biochemical tests. From live fish samples, Escherichia coli was the dominant species with 15 isolates followed by Edwardsiella tarda (12), Salmonella Paratyphi (10), Salmonella Typhi (9), Shigella dysenteriae(7), Shigella flexneri (7), Klebsiella pneumonia (7), Enterobacter aerogenes (6), Enterobacter cloacae (5), Pseudomonas aeruginosa (5), Vibrio parahemolyticus (5), Aeromonas sobria (4), Citrobacter freundii (4), Citrobacter koseri (4) and Plesiomonas shigelloides(3). Detection of common fecal coliforms (E. coli, K. pneumoniae and E. aerogenes) and Salmonella spp. in processed fish indicates the potential danger of passage of pathogenic bacteria and/or their poisons to humans via infected and/or contaminated fish products. Human infection by pathogenic fish bacteria and food poisoning is possible through contamination of fish product in fish production chain due to inadequate handling, poor hygiene and contact with contaminated water. Therefore, producers, consumers and all other stakeholders need to be cautious during handling, processing and consumption of fish harvested from the study lakes.

Modern ecological and hygienic problems of the agricultural economy associated with development of pond fish farming

The materials on the study of the content of heavy metals and organochlorine pesticides in fish are presented. It has been established that the level of heavy metals and pesticides in fish is influenced by the territorial location of the pond production. Therefore, the content of lead, cadmium and mercury in the liver and gonads of carp was higher than the permissible values for fish growing in ponds located in the city limits near highways. The pesticides DDT and HCH were detected in the organs of fish in the territories of pond fish farming of farms. Residual concentrations of pesticides contained in fish did not exceed the maximum permissible levels. In order to safely consume fish grown near highways by the population, we recommend the removal of internal organs (liver and gonads) from fish products.

Digital marketing to increase sales of salted fish products in Muara Badak Ilir village, Kutai Kartanegara

The development of information technology has grown rapidly and has an impact on society in supporting various large and small-scale business activities. Digital Marketing is one of the means to sell that can help increase product sales compared to conventional sales. This community service aims to provide training and assistance to members of the Rukun Nelayan Toko Lima, Muara Badak Ilir Village, Kutai Kartanegara Regency in marketing tembang and anchovy salted fFish products as regional superior products. This program was carried out through a Focus Group Discussion (FGD) to explore problems in product marketing as well as training and assistance in website management, design and marketing of tembang and anchovy salted fish products. The results of the program show that members of the Rukun Nelayan Toko Lima can skillfully manage marketing through digital marketing and website.

Analysis of the Bioprotective Potential of Different Lactic Acid Bacteria Against Listeria monocytogenes in Cold-Smoked Sea Bass, a New Product Packaged Under Vacuum and Stored at 6 ± 2°C

The aim of the work was to monitor the presence of Listeria monocytogenes in cold-smoked fish products (trout, salmon, and sea bass) marketed in Italy. Cold-smoked sea bass is a new product that has not yet been commercialized and was collected from the production facility. Monitoring data have shown that cold-smoked products can be contaminated by L. monocytogenes, the presence of which has been highlighted mainly by enrichment culture (presence in 25 g). The isolated Listeria were serotyped and belonged mainly to low-virulence serotypes (1/2c), followed by serotypes 1/2a, 1/2b, and 4b. Furthermore, considering the ability of L. monocytogenes to grow in these products due to their chemical–physical characteristics (pH &gt; 6.0, Aw &gt; 0.97) and long shelf life at 4°C, an additional aim was to verify the activity of different bioprotective starters, including Lactilactobacillus sakei (LAK-23, Sacco srl, Via Alessandro Manzoni 29/A, 22071 Cadorago, CO, Italy), Carnobacterium spp., Lacticaseibacillus casei (SAL 106), and Lacticaseibacillus paracasei (SAL 211), in cold-smoked sea bass. All starters were bacteriocin producers. For this experiment, smoked sea bass samples were intentionally inoculated with a mixture of three different strains of L. monocytogenes and of each starter culture. After inoculation, the smoked sea bass were vacuum-packed and stored at 6 ± 2°C for 60 days, simulating the typical abuse storage temperature of markets and home refrigerators. At 0, 15, 30, 45, and 60 days, the sea bass samples were analyzed to evaluate the effectiveness of the starters against L. monocytogenes. Listeria monocytogenes growth was prevented only by the addition of the LAK-23 starter. Indeed, at the end of the shelf life, the amount of L. monocytogenes observed was similar to that in the inoculum. Consequently, the use of this starter can allow the inclusion of cold-smoked sea bass or smoked fish products in category 1.3 of Regolamento CE 2073/2005, which are products that do not support the growth of this microorganism. Finally, the activity of the LAK-23 starter did not produce an off flavor or off odor in the smoked sea bass.

Trends in development of Russian Far East’s fishing industry in context of strategic regional growth

This article is devoted to topical issues of the fishing industry development in the Russian Far East. Far East is the largest and the most abundant with fish resources region of Russia, and also it is the largest territorial administrative unit. The main scientific and practical task the research can help to solve is implementation of strategic prospects and scientific support of the industry development government programs. The aim of the research is to update the trends and identify the main problems of the fishing industry development in the Far East of Russia over the past decade. Methodologically the research is based on the concept of regional economy sustainable development. The article analyzes such indicators of the industry development as: number of enterprises in the industry, number of employees at the industry enterprises, volume of fish products production and processing, level of domestic prices for fish products, fish products consumption volume, fish products export value, volume of investments in fixed assets of industry enterprises. The key trends are identified and the most important problems of the regional fishing industry development are noted. Among such problems: the industry’s dependence on the world market situation, insufficient development of domestic market, excessive growth in prices for fish products in the domestic market, low degree of fish products processing, dependence of fish products export on Asian countries’ consumers.

Quality and methods of adulteration of meat and fish products on the Polish market in 2010-2020

Background. Globalisation is the direct or indirect source and cause of many economic, social, political and cultural processes and phenomena. These processes also affect agribusiness and food production. One of the important developments in recent decades is the ever-increasing scale of food adulteration. Its consequence is a reduction in the level of food safety, both in its health and economic aspects. The latter is due to the presence of impaired, or even adulterated, food on the market, which exposes consumers to non-equivalent exchanges. Sectors particularly vulnerable to these illegal trade practices include meat and fish products. Objective. The aim of the article is to identify the dominating methods/categories of food adulteration using the example of the Polish market for the food production sectors of meat and fish. Material and methods. The research was conducted using data from the Agricultural and Food Quality Inspection (AFQI), the official food control institution responsible for quality and food adulteration controls in Poland. The study covered the period from 2010 to 2020. Results. Research has shown an exceptionally significant level and diversity of methods of adulterating meat and fish products. These products are adulterate much more often than other food products. The conducted research has shown many methods and ways of adulterating both meat and fish products. It is worth emphasizing that the greater concentration of counterfeiting methods concerns meat products. The study revealed an extremely significant variety of adulterating methods for meat and fish products, significantly beyond those typically cited in the literature. Conclusions. The results of the research indicate the need to intensify official food controls on the Polish market. In particular, this should apply to the group of meat and fish products due to their high level of adulteration.

Monitoring of productive and biochemical parameters of young Clarias gariepinus during feeding of Skretting and Roycher AQUA feeds in experimental conditions

The article presents the results of monitoring the productive and biochemical indicators of the growth rate of young clary catfish using dry extruded complete feed for fish, which have proven to be best adapted for use at all stages of growing clary catfish, namely: Skretting and Roycher AQUA in experimental conditions. The research was conducted in the educational and experimental laboratories of Bila Tserkva National Agrarian University from 2019 to 2021. For the experiment, we used the young of the African marble clary catfish (Clarias gariepinus), purchased from one of the fish farms in the Odesa region. Clary catfish fry was kept in four rectangular plastic containers for 90 liters of freshwater. The tanks were two-thirds full (60 liters). The water temperature was maintained at 26 °C. For the experiment, 120 fries were used, which were kept in four plastic tanks by the method of analogs of 30 specimens each. Given that the fry differed in weight (for experiment 1 we selected fry with higher weight, and for experiment 2 – with lower weight), they were divided on the principle of analogs into two experimental and two control groups. Based on the results of monitoring in Experiment 1, fish observations, and mathematical calculation, we found that the feed ratio when fed Skretting and Roycher AQUA starter feed is 0.74 and 0.99 %, respectively. Based on the results of monitoring, fish observations, and mathematical calculation, it was found that the feed ratio in experiment 2 when feeding starter feed Skretting and Roycher AQUA is 0.75 and 1.54 %, respectively. The high feed rate for the use of Roycher AQUA can be explained by overuse due to the grinding of the granules. Growing aquaculture facilities in an artificially formed system – production, allows you to get environmentally friendly fish products all year round. This is a very important criterion in modern environmental conditions.

Method of assessing the economic availability of fish products to ensure food security

Продовольственная безопасность помимо прочего означает доступность продуктов питания для всех членов общества. Исходя из этого, одна из задач управления продовольственной безопасностью состоит в обеспечении такого режима функционирования рыночного механизма, при котором достигается баланс между спросом и предложением продовольствия на уровне общественно приемлемых норм его потребления. Целью статьи является совершенствование технологии и инструментов управления продовольственной безопасностью РФ в той их части, которая предназначена для оценки состояния и разработки мероприятий по повышению экономической доступности рыбных товаров. Методология исследования базируется на сочетании теоретических положений концепции благосостояния и микроэкономического анализа. В работе исследуются закономерности спроса на рыбные товары, служащие оценке их доступности с позиции частного и общего благосостояния. Для этого используется модель расчета экономического излишка потребителя, позволяющая в явном виде оценивать экономическую доступность рыбных товаров. На основании полученных результатов предлагаются возможные направления принятия управленческих решений в сфере продовольственной безопасности, нацеленные на повышение доступности рыбных товаров. Food security means the availability of food for everyone in society, among other things. Therefore, one of the goals of food security management is to ensure the functioning of the market mechanism, when a balance is achieved between the demand and supply of food and a socially acceptable level of its consumption is ensured.. The purpose of the paper is to develop food security management tools in the Russian Federation designed to assess the state and develop recommendations for improving the economic availability of fish products. The research methodology is based on a combination of the concept of welfare and microeconomic analysis, when economic accessibility is characterized by certain market parameters. The paper examines models of demand for fish products, which then serve to assess accessibility from the point of view of private and general welfare using the model of economic surplus. The obtained results allowed us to propose directions for making managerial decisions in the field of food security aimed at increasing the availability of fish products.