scholarly journals Phosphorylation of ribosomal protein S6 by cAMP-dependent protein kinase and mitogen-stimulated S6 kinase differentially alters translation of globin mRNA.

1987 ◽  
Vol 262 (8) ◽  
pp. 3518-3523 ◽  
Author(s):  
E. Palen ◽  
J.A. Traugh
Keyword(s):  
Protein Kinase ◽  
Ribosomal Protein ◽  
Globin Mrna ◽  
Dependent Protein Kinase ◽  
S6 Kinase ◽  
Camp Dependent Protein Kinase ◽  
Ribosomal Protein S6 ◽  
Dependent Protein

PKA from Saccharomyces cerevisiae can be activated by cyclic AMP and cyclic GMP

1999 ◽  
Vol 45 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Malgorzata Cytrynska ◽  
Iwona Wojda ◽  
Magdalena Frajnt ◽  
Teresa Jakubowicz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Ribosomal Protein ◽  
Cyclic Gmp ◽  
Enzyme Activation ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Dependent Protein ◽  
Camp And Cgmp

Analysis of Saccharomyces cerevisiae genome revealed no sequence homologous to cyclic GMP (cGMP) dependent protein kinase from other organisms. Here we demonstrate that cyclic AMP (cAMP) dependent protein kinase purified from S. cerevisiae was almost equally activated by cAMP and cGMP at 3 × 10-6 M concentrations of either nucleotide in the presence of Mg2+ ions. Interestingly, if Mn2+ ions were used instead of Mg2+, cGMP was only 30% as effective as cAMP in the activation of cAMP-dependent protein kinase. Analogs of cAMP such as 8-chloro-cAMP and 3':5'-cyclic monophosphate of ribofuranosylbenzimidazole were as potent as cAMP in the enzyme activation, while N6,2'-O-dibutyryl-cAMP activated the enzyme to a lower extent. It was also found that yeast cAMP-dependent protein kinase can be activated by limited proteolytic digestion. The results presented were obtained with protamine and ribosomal protein S10 used as phosphorylation substrates.Key words: cAMP-dependent protein kinase, cAMP, cGMP, yeast, ribosomal protein S10.

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