scholarly journals Effect of Urea on the Divalent Cation Binding Site of Pyruvate Kinase

1971 ◽  
Vol 246 (13) ◽  
pp. 4363-4365 ◽  
Author(s):  
Gene L. Cottam ◽  
Albert S. Mildvan
Keyword(s):  
Binding Site ◽  
Pyruvate Kinase ◽  
Divalent Cation ◽  
Cation Binding ◽  
Cation Binding Site

scholarly journals Chemical properties of the divalent cation binding site on potassium channels.

1992 ◽  
Vol 100 (2) ◽  
pp. 181-193 ◽  
Author(s):  
S Spires ◽  
T Begenisich
Keyword(s):  
Potassium Channels ◽  
Binding Site ◽  
Divalent Cation ◽  
Divalent Cations ◽  
Chemical Properties ◽  
Cation Binding ◽  
Solution Ph ◽  
Electrophysiological Properties ◽  
Cation Binding Site ◽  
Voltage Gated Ion Channels

The actions of divalent cations on voltage-gated ion channels suggest that these cations bind to specific sites and directly influence gating kinetics. We have examined some chemical properties of the external divalent cation binding sites on neuronal potassium channels. Patch clamp techniques were used to measure the electrophysiological properties of these channels and Zn ions were used to probe the divalent cation binding site. The channel activation kinetics were greatly (three- to fourfold) slowed by low (2-5 mM) concentrations of Zn; deactivation kinetics were only slightly affected. These effects of Zn were inhibited by low solution pH in a manner consistent with competition between Zn and H ions for a single site. The apparent inhibitory pK for this site was near 7.2. Treatment of the neurons with specific amino acid reagents implicated amino, but no histidyl or sulfhydryl, residues in divalent cation binding.

scholarly journals Experimental and Theoretical Characterization of the High-Affinity Cation-Binding Site of the Purple Membrane

1998 ◽  
Vol 75 (2) ◽  
pp. 777-784 ◽  
Author(s):  
Leonardo Pardo ◽  
Francesc Sepulcre ◽  
Josep Cladera ◽  
Mireia Duñach ◽  
Amílcar Labarta ◽  
...  
Keyword(s):  
Binding Site ◽  
Purple Membrane ◽  
Cation Binding ◽  
High Affinity ◽  
Cation Binding Site ◽  
Theoretical Characterization
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