scholarly journals Effects of Denaturing Agents on the Phenylalanyl Circular Dichroism Bands of Horseradish Peroxidase Isoenzymes and Apoisoenzymes

1970 ◽  
Vol 245 (6) ◽  
pp. 1233-1238 ◽  
Author(s):  
E. Hardin Strickland ◽  
Ernest Kay ◽  
Leland M. Shannon
Keyword(s):  
Circular Dichroism ◽  
Horseradish Peroxidase ◽  
Peroxidase Isoenzymes ◽  
Circular Dichroïsm

scholarly journals Concanavalin A photocross-linked affinity cryogels for the purification of horseradish peroxidase

2018 ◽  
Vol 36 (3-4) ◽  
pp. 1199-1212 ◽  
Author(s):  
Rüstem Keçili ◽  
Umut Çelikoğlu ◽  
Sevgi Mil ◽  
Arzu Ersöz ◽  
Rıdvan Say
Keyword(s):  
Circular Dichroism ◽  
Horseradish Peroxidase ◽  
Concanavalin A ◽  
Crude Extract ◽  
Cross Linking ◽  
Efficient Procedure ◽  
Sds Page ◽  
Circular Dichroïsm

The present study describes an easy and efficient procedure for the purification of horseradish peroxidase from horseradish roots. For this purpose, supermacroporous cryogels having Concanavalin A were prepared by photosensitive cross-linking polymerization. Horseradish peroxidase binding and elution from the prepared cryogels were carried out changing various parameters such as initial peroxidase concentration and pH. The best binding performance was obtained at pH 7.0. The maximum horseradish peroxidase binding of the cryogels was found to be 3.85 mg g−1 cryogel. Horseradish peroxidase purification from crude extract resulted in 115.1-fold. SDS-PAGE analysis and circular dichroism measurements indicated that the horseradish peroxidase purification from horseradish roots was successfully carried out.

scholarly journals A comparison by magnetic circular dichroism of compound X and compound II of horseradish peroxidase

FEBS Letters ◽  
1987 ◽  
Vol 214 (2) ◽  
pp. 347-350 ◽  
Author(s):  
Nicholas Foote ◽  
Paul M.A. Gadsby ◽  
Robert A. Field ◽  
Colin Greenwood ◽  
Andrew J. Thomson
Keyword(s):  
Circular Dichroism ◽  
Horseradish Peroxidase ◽  
Magnetic Circular Dichroism ◽  
Compound Ii ◽  
Circular Dichroïsm

Characterization of Tamm-Horsfall Urinary Glycoprotein

1973 ◽  
Vol 31 ◽  
pp. 420-421
Author(s):  
John P. Robinson ◽  
J. David Puett
Keyword(s):  
Electron Microscopy ◽  
Circular Dichroism ◽  
Secondary Structure ◽  
Quaternary Structure ◽  
Normal Adult ◽  
Morphological Properties ◽  
Adult Males ◽  
Circular Dichroïsm ◽  
Urinary Glycoprotein

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.

Circular dichroism spectra of adsorbed dye-aggregates

1968 ◽  
Vol 65 ◽  
pp. 146-151 ◽  
Author(s):  
G. Scheibe ◽  
O. Wörz ◽  
F. Haimerl ◽  
W. Seiffert ◽  
J. Winkler
Keyword(s):  
Circular Dichroism ◽  
Circular Dichroism Spectra ◽  
Circular Dichroïsm
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