Novel Binding Partners for CCT and PhLP1 Suggest a Common Folding Mechanism for WD40 Proteins with a 7-Bladed Beta-Propeller Structure

This study investigates whether selected WD40 proteins with a 7-bladed β-propeller structure, similar to that of the β subunit of the G protein heterotrimer, interact with the cytosolic chaperonin CCT and its known binding partner, PhLP1. Previous studies have shown that CCT is required for the folding of the Gβ subunit and other WD40 proteins. The role of PhLP1 in the folding of Gβ has also been established, but it is unknown if PhLP1 assists in the folding of other Gβ-like proteins. The binding of three Gβ-like proteins, TBL2, MLST8 and CDC20, to CCT and PhLP1, was demonstrated in this study. Co-immunoprecipitation assays identified one novel binding partner for CCT and three new interactors for PhLP1. All three of the studied proteins interact with CCT and PhLP1, suggesting that these proteins may have a folding machinery in common with that of Gβ and that the well-established Gβ folding mechanism may have significantly broader biological implications than previously thought. These findings contribute to continuous efforts to determine common traits and unique differences in the folding mechanism of the WD40 β-propeller protein family, and the role PhLP1 has in this process.

Fractured reservoir distribution characterization using folding mechanism analysis and patterns recognition in the Tabnak hydrocarbon reservoir anticline

Naturally, fractured reservoirs play a considerable part in the study, production, and development of hydrocarbon fields because most hydrocarbon reservoirs in the Zagros Basin are naturally fractured. Production from those reservoirs is usually affected by the presence of a system of connected fractures. In this study, the Tabnak hydrocarbon field on the fold–thrust belt at the Zagros zone in the Persian plate has been analyzed by the facies models, folding mechanism analysis to identify fracture reservoir patterns. The results show a flexural fold with similarity in the folding mechanism and some open fracture potential made by limestone, shale, clay, and anhydrite in the study area's facies models. Consequently, the stress pattern and type of fracture issue on the fold's upper and lower layers will be similar. On the Tabnak anticline reservoir using image processing techniques in MATLAB R2019 software and kriging geostatistical methods, fracture surface patterns as a block model extended to the depth. Using the model results, fractures’ orientation distribution in adjacent wells 11, 14, and 15 is appropriate. The results also have similarities with the facies models, folding mechanism assessment, well test, and mud loss data analysis. These results can affect the development plans’ primary approach by drilling horizontal and sleep wells and hydrocarbon reservoir management strategies.

Design and Fabrication of Wheelchair CUM Stretcher

The wheelchair and stretcher are very widely used in hospitals, airports, train stations, shopping malls, etc. This design here is a modified wheelchair stretcher as needed. This machine converts the wheelchair into a stretcher. The chair transforms into a stretcher when the levers are operated. The stretchers can be detached from the main frame according to the convenience of the patient and doctors can make it easier Access to the patient with less effort and transport The folding mechanism enables a large number of stretchers arranged in chair form to be accommodated in a comparatively smaller space. in hospitals, patients have to be moved from a wheelchair to a stretcher, from a stretcher to a bed, from a bed to a wheelchair or vice versa; which creates unsafe conditions for patients. A wheelchair stretcher is required to facilitate mobility for the disabled patient.