scholarly journals Kinetic characterization of the normal and detergent-perturbed reaction cycles of the sarcoplasmic reticulum calcium pump. Rate-limiting step(s) under different conditions.

1986 ◽  
Vol 261 (35) ◽  
pp. 16372-16384
Author(s):  
P Champeil ◽  
M le Maire ◽  
J P Andersen ◽  
F Guillain ◽  
M Gingold ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Pump ◽  
Kinetic Characterization ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Pump Rate ◽  
Reaction Cycles ◽  
Rate Limiting ◽  
Sarcoplasmic Reticulum Calcium

scholarly journals Kinetic characterization of the normal and procaine-perturbed reaction cycles of the sarcoplasmic reticulum calcium pump

1991 ◽  
Vol 202 (2) ◽  
pp. 559-567 ◽  
Author(s):  
Fernando HENAO ◽  
Beatrice FORESTA ◽  
Stephane ORLOWSKI ◽  
Ana CUENDA ◽  
Carlos GUTIERREZ-MERINO ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Pump ◽  
Kinetic Characterization ◽  
Reaction Cycles ◽  
Sarcoplasmic Reticulum Calcium

Wavelet Based Structural Analysis of Electroless Deposits in the Diffusion Limited Regime

1994 ◽  
Vol 367 ◽  
Author(s):  
A. Arneodo ◽  
F. Argoul ◽  
A. Kuhn ◽  
J.F. Muzy
Keyword(s):  
Electroless Deposition ◽  
Side Reactions ◽  
Statistical Features ◽  
Diffusion Limited ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Fractal Patterns ◽  
Rate Limiting ◽  
Comparative Structural Characterization

AbstractWe discuss the actual relevance of thin gap geometry electrodeposition to generate fractal patterns that mimic the morphology of Witten and Sander's diffusion-limited aggregates (DLA). Eliminating migration and convection, as well as electrochemical side reactions, we show that electroless deposition is a good candidate to meet the requirements for diffusion to be the rate limiting step of the growth process. We use the wavelet transform microscope to achieve a comparative structural characterization of both experimental electroless deposits and numerical DLA clusters. The fact that five-fold symmetry and Fibonacci hierarchical ordering are found as common predominant statistical features is, to our knowledge, the first demonstration, relying on an appropriate structural fractal analysis, of the existence of DLA morphologies in an experimental context.

Phosphorylation of Ca-ATPase of Sarcoplasmic Reticulum with Different Substrates

1983 ◽  
Vol 38 (9-10) ◽  
pp. 845-847 ◽  
Author(s):  
Hector Barrabin ◽  
Leopoldo de Meis
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Low Temperatures ◽  
Hplc Analysis ◽  
Calcium Concentration ◽  
Specific Activity ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
High Calcium ◽  
High Calcium Concentration ◽  
Rate Limiting

ATP and GTP as substrate for phosphorylation of sarcoplasmic reticulum ATPase are compared. Maximal levels of phosphoenzyme are between 4.5 and 4.8 nmol per mg of protein when either substrate is used provided that phosphoenzyme hydrolysis are strongly inhibited by high calcium concentration (20 mм) and low temperatures ( 0 ° C ) . The maximal values obtained with GTP are lower than those previously reported. It is shown that this difference is due to underestimation of the specific activity of labeled nucleotides used in previous studies, as revealed by UV absorption and HPLC analysis. The dependence of the phosphoenzyme levels on calcium concentration, pH and temperature confirm previous findings indicating that ATP, but no GTP, accelerates the rate limiting step of the catalytic cycle.

Ornithine Decarboxylase Activity in Cultured Endothelial Cells Stimulated by Serum, Thrombin and Serotonin

1978 ◽  
Vol 39 (02) ◽  
pp. 496-503 ◽  
Author(s):  
P A D’Amore ◽  
H B Hechtman ◽  
D Shepro
Keyword(s):  
Endothelial Cells ◽  
Ornithine Decarboxylase ◽  
Decarboxylase Activity ◽  
Aortic Endothelial Cells ◽  
Ornithine Decarboxylase Activity ◽  
Rate Limiting Step ◽  
Bovine Aortic Endothelial Cells ◽  
Limiting Step ◽  
Rate Limiting ◽  
Serum Serotonin

SummaryOrnithine decarboxylase (ODC) activity, the rate-limiting step in the synthesis of polyamines, can be demonstrated in cultured, bovine, aortic endothelial cells (EC). Serum, serotonin and thrombin produce a rise in ODC activity. The serotonin-induced ODC activity is significantly blocked by imipramine (10-5 M) or Lilly 11 0140 (10-6M). Preincubation of EC with these blockers together almost completely depresses the 5-HT-stimulated ODC activity. These observations suggest a manner by which platelets may maintain EC structural and metabolic soundness.

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