scholarly journals Escherichia coli dGTP triphosphohydrolase is inhibited by gene 1.2 protein of bacteriophage T7.

1988 ◽  
Vol 263 (27) ◽  
pp. 13549-13556 ◽  
Author(s):  
H E Huber ◽  
B B Beauchamp ◽  
C C Richardson
Keyword(s):  
Escherichia Coli ◽  
Bacteriophage T7

scholarly journals An Endonuclease Induced after Infection of Escherichia coli with Bacteriophage T7

1970 ◽  
Vol 245 (23) ◽  
pp. 6292-6299 ◽  
Author(s):  
Melvin S. Center ◽  
Charles C. Richardson
Keyword(s):  
Escherichia Coli ◽  
Bacteriophage T7

Development of T7 phage and T7 phage containing apurinic sites in an exonuclease III, endonuclease IV double mutant of Escherichia coli

1992 ◽  
Vol 70 (7) ◽  
pp. 605-608 ◽  
Author(s):  
Giselle Sanchez ◽  
Margaret D. Mamet-Bratley
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Dna Synthesis ◽  
Double Mutant ◽  
Bacteriophage T7 ◽  
Bacterial Dna ◽  
Exonuclease Iii ◽  
Repair Enzymes ◽  
Phage Dna ◽  
T7 Phage

The development of bacteriophage T7 was examined in an Escherichia coli double mutant defective for the two major apurinic, apyrimidinic endonucleases (exonuclease III and endonuclease IV, xth nfo). In cells infected with phages containing apurinic sites, the defect in repair enzymes led to a decrease of phage survival and a total absence of bacterial DNA degradation and of phage DNA synthesis. These results directly demonstrate the toxic action of apurinic sites on bacteriophage T7 at the intracellular level and its alleviation by DNA repair. In addition, untreated T7 phage unexpectedly displayed reduced plating efficiency and decreased DNA synthesis in the xth nfo double mutant.Key words: apurinic sites, DNA repair, T7 phage.

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