Purification and Properties of Interstitial Cell-stimulating Hormone from Sheep Pituitary Glands

SUMMARY Four gonadotrophic fractions from human pituitary glands have been assayed in terms of the International Reference Preparation, HMG 24, by four different methods. These are the assay for total gonadotrophin which depends on the increase in uterine weight of the immature mouse, the augmentation method for follicle-stimulating hormone, the increase in weight of the ventral prostate of the hypophysectomized rat for interstitial cell-stimulating hormone and the ovulation method for the 'ovulating ' factor. The results have been contrasted and discussed.

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PURIFICATION AND PROPERTIES OF THE FOLLICLE-STIMULATING HORMONE INHIBITOR OBTAINED FROM THE URINE OF THE BONNET MONKEY

Journal of Endocrinology ◽

10.1677/joe.0.0400165 ◽

1968 ◽

Vol 40 (2) ◽

pp. 165-173 ◽

Cited By ~ 3

Author(s):

M. R. SAIRAM ◽

H. G. MADHWA RAJ ◽

N. R. MOUDGAL

Keyword(s):

Follicle Stimulating Hormone ◽

Gel Filtration ◽

Deae Cellulose ◽

Selective Extraction ◽

Human Pituitary ◽

Ammonium Sulphate Precipitation ◽

Purification And Properties ◽

Rat Pituitary ◽

Pituitary Glands ◽

Stimulating Hormone

SUMMARY The follicle-stimulating hormone (FSH) inhibitor in monkey urine was purified by selective extraction of the crude extract with acetate buffer, ammonium sulphate precipitation and DEAE-cellulose chromatography. The purified inhibitor was free of luteinizing hormone activity. It behaved as an apparently homogeneous protein. The inhibitor contained about 20% carbohydrate (hexoses, hexosamines, fucose and sialic acid). Thin-layer gel filtration indicated a molecular weight of about 65,000. The inhibitor was labile to heat treatment, exposure to extremes of pH and denaturing agents. The inhibitor effectively neutralized the biological activity of FSH preparations from human, monkey, horse, pig, sheep and rat pituitary glands, pregnant mare serum gonadotrophin and human pituitary urinary gonadotrophin.

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An autoradiographic study of the intracellular transport of interstitial cell stimulating hormone in dissociated pituitary glands of normal and castrated rats

10.31274/rtd-180814-2057 ◽

1978 ◽

Author(s):

John Gunnar Linner

Keyword(s):

Intracellular Transport ◽

Interstitial Cell ◽

Autoradiographic Study ◽

Pituitary Glands ◽

Stimulating Hormone

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LACK OF AROMATISATION OF CIRCULATING DEHYDROEPIANDROSTERONE AND DEHYDROEPIANDROSTERONE SULPHATE IN AMENORRHOEIC WOMEN STIMULATED WITH HUMAN GONADOTROPHINS

Acta Endocrinologica ◽

10.1530/acta.0.0490248 ◽

1965 ◽

Vol 49 (2) ◽

pp. 248-261 ◽

Cited By ~ 8

Author(s):

S. Mancuso ◽

Francesca P. Mancuso ◽

K.-G. Tillinger ◽

E. Diczfalusy

Keyword(s):

Pregnant Women ◽

Ovarian Stimulation ◽

Interstitial Cell ◽

Follicular Phase ◽

Radioactive Material ◽

Dehydroepiandrosterone Sulphate ◽

Experimental Conditions ◽

Human Menopausal Gonadotrophin ◽

Urine Specimens ◽

Stimulating Hormone

ABSTRACT Two amenorrhoeic women were given a course of 10 injections of human menopausal gonadotrophin (HMG) in daily doses corresponding to 260 IU of follicle stimulating hormone (FSH) activity and 165 IU of interstitial cell stimulating hormone (ICSH) activity. In both patients an extensive ovarian stimulation was observed as indicated by the greatly increased urinary excretion of oestrone, 17β-oestradiol and oestriol. When HMG-treatment was followed subsequently by the administration of human chorionic gonadotrophin (HCG) for 5 days in a total dose of 18 000 and 30 000 IU, respectively, functional corpus luteum tissue was formed in both patients as evidenced by a huge rise in urinary pregnane-3α,20α-diol excretion and by the secretory transformation of a previously atrophic endometrium. At the approximate height of the follicular phase tracer doses of 3H-labelled dehydroepiandrosterone sulphate (DHAS) and 14C-labelled dehydroepiandrosterone (DHA) were administered to both patients in the form of a continuous intravenous infusion of 10 hours' duration. Infusion of the same dose was repeated under identical experimental conditions at the approximate height of the luteal phase. In both patients, very little radioactive material was associated with oestrone and 17β-oestradiol and none with oestriol isolated from 96-hours' urine specimens obtained at both phases of ovarian stimulation. It is concluded that — in contradistinction to the situation in pregnant women — circulating DHAS is not a significant precursor of urinary oestrogens in non-pregnant women.

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