scholarly journals Deletion analysis of the mini-P1 plasmid origin of replication and the role of Escherichia coli DnaA protein.

1990 ◽  
Vol 265 (20) ◽  
pp. 11622-11627
Author(s):  
S Wickner ◽  
J Hoskins ◽  
D Chattoraj ◽  
K McKenney
Keyword(s):  
Escherichia Coli ◽  
Deletion Analysis ◽  
Origin Of Replication ◽  
Dnaa Protein

scholarly journals Role of RepA and DnaA Proteins in the Opening of the Origin of DNA Replication of an IncB Plasmid

2004 ◽  
Vol 186 (12) ◽  
pp. 3785-3793 ◽  
Author(s):  
T. Betteridge ◽  
J. Yang ◽  
A. J. Pittard ◽  
J. Praszkier
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Origin Of Replication ◽  
Initiator Protein ◽  
Origin Of Dna Replication ◽  
Replication Initiator Protein ◽  
Replication Initiator

ABSTRACT The replication initiator protein RepA of the IncB plasmid pMU720 was shown to induce localized unwinding of its cognate origin of replication in vitro. DnaA, the initiator protein of Escherichia coli, was unable to induce localized unwinding of this origin of replication on its own but enhanced the opening generated by RepA. The opened region lies immediately downstream of the last of the three binding sites for RepA (RepA boxes) and covers one turn of DNA helix. A 6-mer sequence, 5′-TCTTAA-3′, which lies within the opened region, was essential for the localized unwinding of the origin in vitro and origin activity in vivo. In addition, efficient unwinding of the origin of replication of pMU720 in vitro required the native positioning of the binding sites for the initiator proteins. Interestingly, binding of RepA to RepA box 1, which is essential for origin activity, was not required for the localized opening of the origin in vitro.

Role of DnaA protein during replication of plasmid pBR322 in Escherichia coli

1991 ◽  
Vol 225 (3) ◽  
pp. 435-442 ◽  
Author(s):  
C. -S. Chiang ◽  
Y. -C. Xu ◽  
H. Bremer
Keyword(s):  
Escherichia Coli ◽  
Plasmid Pbr322 ◽  
Dnaa Protein
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