scholarly journals Induction of Aryl Hydrocarbon (Benzo[a]pyrene) Hydroxylase in Liver Cell Culture by Temporary Inhibition of Protein Synthesis

1973 ◽  
Vol 248 (17) ◽  
pp. 6114-6121
Author(s):  
James P. Whitlock ◽  
Harry V. Gelboin
Keyword(s):  
Cell Culture ◽  
Protein Synthesis ◽  
Liver Cell ◽  
Aryl Hydrocarbon ◽  
Liver Cell Culture ◽  
Inhibition Of Protein Synthesis ◽  
Pyrene Hydroxylase ◽  
Temporary Inhibition

scholarly journals Evidence that ligand formation is a mechanism underlying the maintenance of cytochrome P-450 in rat liver cell culture. Potent maintenance by metyrapone

1980 ◽  
Vol 188 (3) ◽  
pp. 937-939 ◽  
Author(s):  
A J Paine ◽  
P Villa ◽  
L J Hockin
Keyword(s):  
Cell Culture ◽  
Protein Synthesis ◽  
Rat Liver ◽  
Liver Cell ◽  
Rat Hepatocytes ◽  
Liver Cell Culture ◽  
Substituted Pyridines ◽  
Cultured Rat Hepatocytes ◽  
Highly Correlated ◽  
Cytochrome P 450

The loss of cytochrome P-450 in cultured rat hepatocytes can be prevented by substituted pyridines, especially isonicotinamide, 3-hydroxypyridine and metyrapone. The effect of these compounds is independent of protein synthesis, suggesting that they maintain pre-existing cytochrome P-450. The efficiency of pyridines at maintaining cytochrome P-450 in hepatocyte culture is highly correlated with their ability to bind to this cytochrome, suggesting that ligand formation with cytochrome P-450 prevents its accelerated turnover in liver cell culture.

Sign in / Sign up

Export Citation Format

Share Document