Determination of pyruvate and lactate in primary liver cell culture medium during hypoxia by on-line microdialysis–liquid chromatography

2001 ◽  
Vol 913 (1-2) ◽  
pp. 341-347 ◽  
Author(s):  
Yuh-Shen Wu ◽  
Tung-Hu Tsai ◽  
Tsu-Fang Wu ◽  
Fu-Chou Cheng
Keyword(s):  
Cell Culture ◽  
Liquid Chromatography ◽  
Liver Cell ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Liver Cell Culture ◽  
On Line

scholarly journals Quantitative determination of urea concentrations in cell culture medium

2009 ◽  
Vol 87 (3) ◽  
pp. 541-544 ◽  
Author(s):  
Robert J.X. Zawada ◽  
Peggy Kwan ◽  
Kellen L. Olszewski ◽  
Manuel Llinas ◽  
Shu-Gui Huang
Keyword(s):  
Cell Culture ◽  
Quantitative Determination ◽  
Protein Metabolism ◽  
Room Temperature ◽  
Culture Medium ◽  
Urea Synthesis ◽  
Cell Culture Medium ◽  
Urea Determination ◽  
Sensitive Method

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.

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