Thin-layer gel chromatography of dyed proteins

Kinetic and equilibrium studies were performed on the reactions of the hapten ε-dinitrophenyl-lysine with specific intact antibodies, reduced, alkylated, and polyalanylated antibodies, and reduced, alkylated, and polyalanylated γ-chains. No reaction was detected between the hapten and light chains. The γ-chains were found to have 0.5 combining sites per chain, and thin layer gel chromatography revealed that they existed as monomers. The rate constant of association for the reaction of γ-chains with hapten was found to be almost 1000 times lower than that for the corresponding reaction with the parent antibody. Differences in the rate constants of dissociation were much less pronounced. These results suggested that the combining site in the separated γ-chain had undergone a change in conformation.

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The Fractionation of Beef Brain Ganglioside into Several Components with Thin-Layer and Column Silica Gel Chromatography

Cerebral Sphingolipidoses ◽

10.1016/b978-1-4831-9648-0.50025-0 ◽

1962 ◽

pp. 289-299 ◽

Cited By ~ 6

Author(s):

JOEL A. DAIN ◽

H. WEICKER ◽

GERHARD SCHMIDT ◽

S.J. THANNHAUSER

Keyword(s):

Thin Layer ◽

Silica Gel ◽

Gel Chromatography ◽

Brain Ganglioside ◽

Beef Brain ◽

Silica Gel Chromatography

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Thin-layer gel chromatography of proteins in mild and denaturing detergents

Journal of Chromatography A ◽

10.1016/s0021-9673(00)92337-9 ◽

1978 ◽

Vol 157 ◽

pp. 227-234 ◽

Cited By ~ 7

Author(s):

James F. Collawn ◽

David J. Cox ◽

Larry M. Hamlin ◽

Wayne W. Fish

Keyword(s):

Thin Layer ◽

Gel Chromatography

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Faster estimation of reserve bilirubin binding capacity of serum from the neonate by thin-layer chromatography on sephadex.

Clinical Chemistry ◽

10.1093/clinchem/23.3.541 ◽

1977 ◽

Vol 23 (3) ◽

pp. 541-545 ◽

Cited By ~ 5

Author(s):

F Trivin ◽

M Odièvre ◽

A Lemonnier

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

Binding Capacity ◽

Gel Chromatography ◽

Standard Solution ◽

Bilirubin Binding ◽

Layer Chromatography

Abstract Knowledge of the reserve bilirubin-binding capacity is useful in management of hyperbilirubinemia in the newborn. We describe thin-layer gel chromatography that permits measurement of this variable, with a 250-mul sample of serum, within 50 min. Single samples from four different infants can be analyzed on the same plate. The capacity of 40 sera from jaundiced and of 19 sera from nonjaundiced infants was measured. Results by thin-layer Sephadex gel chromatography and column Sephadex chromatography correlated well. Dithiothreitol, added to the standard bilirubin solution, protected bilirubin from oxidation for at least six days, which obviated the time consuming preparation of standard solution before such determination.

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