Use of centrifugal partition chromatography for assessing partition coefficients in various solvent systems

1989 ◽  
Vol 469 ◽  
pp. 91-99 ◽  
Author(s):  
Nabil El Tayar ◽  
Andrew Marston ◽  
Antoine Bechalany ◽  
Kurt Hostettmann ◽  
Bernard Testa
Keyword(s):  
Partition Coefficients ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Solvent Systems

scholarly journals Centrifugal partition chromatography(CPC) as a useful method for determination of partition coefficients between octanol and water.

1987 ◽  
Vol 35 (12) ◽  
pp. 5010-5014 ◽  
Author(s):  
HIROSHI TERADA ◽  
YASUTAKA KOSUGE ◽  
NOBUYUKI NAKAYA ◽  
WATARU MURAYAMA ◽  
YOSHIAKI NUNOGAKI ◽  
...  
Keyword(s):  
Partition Coefficients ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography

A comprehensive classification of solvent systems used for natural product purifications in countercurrent and centrifugal partition chromatography

2015 ◽  
Vol 32 (11) ◽  
pp. 1556-1561 ◽  
Author(s):  
Krystyna Skalicka-Woźniak ◽  
Ian Garrard
Keyword(s):  
Natural Product ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Solvent Systems ◽  
Comprehensive Classification

This review is a comprehensive classification of solvent systems used for natural product purifications in countercurrent and centrifugal partition chromatography.

scholarly journals Preparative Purification of Delphinidin 3-0-sambubioside from Roselle (Hibiscus sabdariffa L.) Petals by fast Centrifugation Partition Chromatography

2010 ◽  
Vol 6 (2) ◽  
pp. 999-1004
Author(s):  
Hilaire Tanoh Kouakou ◽  
Laurent Kouakou Kouakou ◽  
Alain Decendit ◽  
Alain Badoc ◽  
Gregory Da-Costa ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Amyloid Fibrils ◽  
Hibiscus Sabdariffa ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Preparative Scale ◽  
Mobile Phases ◽  
Chromatographic Procedure ◽  
Solvent Systems ◽  
Liquid Chromatographic

Delphinidin 3-0-sambubioside, a Hibiscus anthocyanin, was isolated from MeOH/TFA dried flower of H. sabdariffa. Its purification on preparative scale was obtained by centrifugal partition chromatography (CPC) using the ternary biphasic solvent systems composed of ethyl acetate/1-butanol/water, acidified by 0.1% of TFA. Stationary phase was ethyl acetate/1-butanol/water (5:5:90; v/v). We tested 4 mobile phases and found that the system acetate/1-butanol/water (40:46:14; v/v) was the best to separate anthocyanin mentioned above. This support-free liquid-liquid chromatographic procedure made it possible to isolate delphinidin 3-0-sambubioside from flower of H. sabdariffa. The antiamyloidogenic activity of the isolated stilbenes was evaluated versus b-amyloid fibrils. delphinidin 3-0-sambubioside was found to be active with 67% inhibition at 10 mM.

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