A comprehensive classification of solvent systems used for natural product purifications in countercurrent and centrifugal partition chromatography

This review is a comprehensive classification of solvent systems used for natural product purifications in countercurrent and centrifugal partition chromatography.

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Characterization and correlation of mobile phase dispersion of aqueous-organic solvent systems in centrifugal partition chromatography

Journal of Chromatography A ◽

10.1016/j.chroma.2020.460990 ◽

2020 ◽

Vol 1620 ◽

pp. 460990

Author(s):

A. Fromme ◽

C. Fischer ◽

K. Keine ◽

G. Schembecker

Keyword(s):

Organic Solvent ◽

Mobile Phase ◽

Partition Chromatography ◽

Centrifugal Partition Chromatography ◽

Phase Dispersion ◽

Solvent Systems

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Use of centrifugal partition chromatography for assessing partition coefficients in various solvent systems

Journal of Chromatography A ◽

10.1016/s0021-9673(01)96443-x ◽

1989 ◽

Vol 469 ◽

pp. 91-99 ◽

Cited By ~ 15

Author(s):

Nabil El Tayar ◽

Andrew Marston ◽

Antoine Bechalany ◽

Kurt Hostettmann ◽

Bernard Testa

Keyword(s):

Partition Coefficients ◽

Partition Chromatography ◽

Centrifugal Partition Chromatography ◽

Solvent Systems

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Comparison of Several Aqueous two Phase Solvent Systems (ATPS) for the Fractionation of Biopolymers by Centrifugal Partition Chromatography (CPC)

Journal of Liquid Chromatography ◽

10.1080/01483919008049043 ◽

1990 ◽

Vol 13 (12) ◽

pp. 2421-2440 ◽

Cited By ~ 13

Author(s):

Alain Foucault ◽

Koji Nakanishi

Keyword(s):

Partition Chromatography ◽

Centrifugal Partition Chromatography ◽

Two Phase ◽

Solvent Systems

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Target-Guided Isolation of O-tigloylcyclovirobuxeine-B from Buxus sempervirens L. by Centrifugal Partition Chromatography

Molecules ◽

10.3390/molecules25204804 ◽

2020 ◽

Vol 25 (20) ◽

pp. 4804

Author(s):

Lara U. Szabó ◽

Thomas J. Schmidt

Keyword(s):

Natural Product ◽

Partition Chromatography ◽

Centrifugal Partition Chromatography ◽

Target Compound ◽

Preparative Scale ◽

Buxus Sempervirens ◽

Life Threatening ◽

Identification And Characterization

The increasing drug resistance of malaria parasites challenges the treatment of this life-threatening disease. Consequently, the development of innovative and effective antimalarial drugs is inevitable. O-tigloylcyclovirobuxeine-B, a nor-cycloartane alkaloid from Buxussempervirens L., has shown promising and selective in vitro activity in previous studies against Plasmodiumfalciparum (Pf), causative agent of Malaria tropica. For further investigations, it is indispensable to develop an advanced and efficient isolation procedure of this valuable natural product. Accordingly, we used liquid–liquid chromatography including centrifugal partition chromatography (CPC) to obtain the pure alkaloid on a semi-preparative scale. Identification and characterization of the target compound was accomplished by UHPLC/+ESI-QqTOF-MS/MS, 1H NMR and 13C NMR. In conclusion, this work provides a new and efficient method to obtain O-tigloylcyclovirobuxeine-B, a valuable natural product, as a promising antiplasmodial lead structure for the development of innovative and safe medicinal agents.

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Preparative Purification of Delphinidin 3-0-sambubioside from Roselle (Hibiscus sabdariffa L.) Petals by fast Centrifugation Partition Chromatography

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v6i2.2628 ◽

2010 ◽

Vol 6 (2) ◽

pp. 999-1004

Author(s):

Hilaire Tanoh Kouakou ◽

Laurent Kouakou Kouakou ◽

Alain Decendit ◽

Alain Badoc ◽

Gregory Da-Costa ◽

...

Keyword(s):

Ethyl Acetate ◽

Amyloid Fibrils ◽

Hibiscus Sabdariffa ◽

Partition Chromatography ◽

Centrifugal Partition Chromatography ◽

Preparative Scale ◽

Mobile Phases ◽

Chromatographic Procedure ◽

Solvent Systems ◽

Liquid Chromatographic

Delphinidin 3-0-sambubioside, a Hibiscus anthocyanin, was isolated from MeOH/TFA dried flower of H. sabdariffa. Its purification on preparative scale was obtained by centrifugal partition chromatography (CPC) using the ternary biphasic solvent systems composed of ethyl acetate/1-butanol/water, acidified by 0.1% of TFA. Stationary phase was ethyl acetate/1-butanol/water (5:5:90; v/v). We tested 4 mobile phases and found that the system acetate/1-butanol/water (40:46:14; v/v) was the best to separate anthocyanin mentioned above. This support-free liquid-liquid chromatographic procedure made it possible to isolate delphinidin 3-0-sambubioside from flower of H. sabdariffa. The antiamyloidogenic activity of the isolated stilbenes was evaluated versus b-amyloid fibrils. delphinidin 3-0-sambubioside was found to be active with 67% inhibition at 10 mM.

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