mRNA-based electrotransfection of human dendritic cells and induction of cytotoxic T lymphocyte responses against the telomerase catalytic subunit (hTERT)

2002 ◽  
Vol 259 (1-2) ◽  
pp. 191-203 ◽  
Author(s):  
Stein Sæbøe-Larssen ◽  
Ellen Fossberg ◽  
Gustav Gaudernack
Keyword(s):  
Dendritic Cells ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Catalytic Subunit ◽  
Human Dendritic Cells ◽  
Lymphocyte Responses

Residual Lymphocytes in GM-CSF and IL-15 Differentiated Monocyte-Derived Dendritic Cells Enables Cytotoxic T Lymphocyte Responses.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4907-4907
Author(s):  
Melinda Y. Hardy ◽  
Andrew J. Kassianos ◽  
Ray Wilkinson ◽  
Annelie Vulink ◽  
Derek N.J. Hart ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Small Population ◽  
Lymphoid Cells ◽  
Gm Csf ◽  
Hla Dr ◽  
Ifn Γ ◽  
Human Dendritic Cells ◽  
Ctl Responses

Abstract We investigated the capacity of IL-15 to differentiate human dendritic cells (DC) from monocytes in the presence of GM-CSF (IL-15 MoDC) and compared them with MoDC differentiated in IL-4 and GM-CSF (IL-4 MoDC) as used in many immunotherapy protocols. IL-15 MoDC expressed higher levels of CD40 and HLA-DR and importantly, induced MART-1 specific cytotoxic T lymphocyte (CTL) responses with superior lytic capacity, when compared to IL-4 MoDC. In response to activation, IL-15 MoDC secreted high levels of IFN-γbut low or no IL-12, whereas IL-4 MoDC secreted high IL-12 but low or no IFN-γ. Using an IFN-γ blocking antibody, we demonstrated that IFN-γ production by the IL-15 MoDC did not account for the superior CTL responses induced. Despite immunoselecting monocytes to greater than 97% purity prior to DC differentiation, we noticed a small population (1–2%) of CD56+ and CD3+ lymphocytes in the IL-15 MoDC preparations that were less prominent in IL-4 MoDC differentiated from the same monocytes. Removal of the residual lymphocytes from monocytes prior to differentiation into IL-15 MoDC diminished their capacity to induce CTL but did not affect the expression of HLA-DR or CD40. These data suggest that IL-15-dependent cross-talk between the small lymphoid populations present and DC, during DC differentiation from monocytes results in superior CTL priming that is independent of IL-12 and IFN-γ. Based on these results, appropriately manufactured IL-15 MoDC preparations containing defined numbers of lymphoid cells should be considered for immunotherapy.

Dendritic Cells and Antigen Presentation in the Regulation of Cytotoxic T Lymphocyte Responses against Viruses and Transplantation Antigens

1988 ◽  
Vol 532 (1 Cytotoxic T C) ◽  
pp. 280-291 ◽  
Author(s):  
CORNELIS J. M. MELIEF ◽  
CLAIRE J. P. BOOG ◽  
WIES L. E. VASMEL ◽  
JOLANDE BOES ◽  
ARIE C. VOORDOUW ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Antigen Presentation ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Lymphocyte Responses ◽  
Transplantation Antigens

scholarly journals Mature dendritic cells pulsed with exosomes stimulate efficient cytotoxic T-lymphocyte responses and antitumour immunity

Immunology ◽  
2007 ◽  
Vol 120 (1) ◽  
Author(s):  
Siguo Hao ◽  
Ou Bai ◽  
Fang Li ◽  
Jinying Yuan ◽  
Suzanne Laferte ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Lymphocyte Responses

Role of macrophages and dendritic cells in primary cytotoxic T lymphocyte responses

1995 ◽  
Vol 7 (4) ◽  
pp. 679-688 ◽  
Author(s):  
Smita Nair ◽  
Antoinette M. J. Buiting ◽  
Richard J. D. Rouse ◽  
Nico Van Rooijen ◽  
Leaf Huang ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Lymphocyte Responses
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