scholarly journals Analytical isoelectric focusing with immobilized pH gradients of human apolipoprotein E from very low density lipoproteins and total plasma.

1990 ◽  
Vol 31 (1) ◽  
pp. 149-155
Author(s):  
F Mailly ◽  
J Davignon ◽  
AC Nestruck
1984 ◽  
Vol 30 (3) ◽  
pp. 349-351 ◽  
Author(s):  
M J Bugugnani ◽  
M Koffigan ◽  
I Kora ◽  
D Ouvry ◽  
V Clavey ◽  
...  

Abstract We describe an isoelectric-focusing method for rapidly separating and quantifying apolipoprotein (apo) C-II and C-III subspecies of triglyceride-rich lipoproteins. Concentrated very-low-density lipoproteins (VLDL) or delipidated apo VLDL are focused on dry acrylamide plates, after their rehydration with ampholytes and urea. Apo C-II and apo C-III in VLDL are resolved into four major bands--C-II (pI 5.01), C-III0 (pI 5.10), C-III1 (pl 4.92), and C-III2 (pI 4.84)--at the same pI values as for purified apo C. This precise technique can be used without delipidating VLDL. The relative percentage of C apoproteins found in VLDL from plasma of normal subjects agreed with previously published data. The ratio of apo C-II to apo C-III decreased in patients with chronic renal failure or with coronary artery disease.


1998 ◽  
Vol 136 (2) ◽  
pp. 255-262 ◽  
Author(s):  
Frits H.A.F de Man ◽  
Femke de Beer ◽  
Arnoud van der Laarse ◽  
Augustinus H.M Smelt ◽  
Jan A.Gevers Leuven ◽  
...  

1989 ◽  
Vol 182 (1) ◽  
pp. 94-97 ◽  
Author(s):  
Germán Camejo ◽  
Birgitta Rosengren ◽  
Urban Olsson ◽  
Göran Bondjers

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