Optimization of a liquid–liquid extraction method for HPLC–DAD determination of penicillin-V in human plasma

2002 ◽  
Vol 72 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Andrei Medvedovici ◽  
Mihaela Ionescu ◽  
Constantin Mircioiu ◽  
Victor David
Keyword(s):  
Human Plasma ◽  
Extraction Method ◽  
Liquid Extraction ◽  
Penicillin V ◽  
Liquid Liquid Extraction

Determination of Flurbiprofen in Human Plasma by Gas Chromatography with Mass Spectrometry and Its Pharmacokinetics

2014 ◽  
Vol 97 (4) ◽  
pp. 1061-1066 ◽  
Author(s):  
Bilal Yilmaz ◽  
Huseyin Sahin ◽  
Vedat Akba ◽  
Ali Fuat Erdem
Keyword(s):  
Mass Spectrometry ◽  
Human Plasma ◽  
Extraction Method ◽  
Internal Standard ◽  
Liquid Extraction ◽  
Pharmacokinetic Parameters ◽  
Liquid Liquid Extraction ◽  
Interday Precision ◽  
Better Than

Abstract This paper describes a GC/MS method for the determination of flurbiprofen in human plasma. Flurbiprofen and internal standard ibuprofen were extracted from plasma by using a liquid–liquid extraction method. Derivatization was carried out using N-Methyl-N-(trimethylsilyl)trifluoroacetamide. The calibration curve was linear between the concentration range of 0.10 and 5.0 μg/mL. Intraday and interday precision values for flurbiprofen in plasma were less than 5.49%, and accuracy (relative error) was better than 5.33%. The extraction recoveries of flurbiprofen from human plasma were between 93.6 and 98.6%. The LOD and LOQ of flurbiprofen were 0.03 and 0.10 μg/mL, respectively. This assay was applied to determine the pharmacokinetic parameters of flurbiprofen in healthy Turkish volunteers who had been given 100 mg of flurbiprofen.

An HPLC Determination of Trimetazidine in Human Plasma Using Liquid‐Liquid Extraction for Sample Clean‐Up

2005 ◽  
Vol 28 (9) ◽  
pp. 1299-1309 ◽  
Author(s):  
Min Kyo Jeoung ◽  
Kyoung Soon Kim ◽  
Chang Soo Kim ◽  
Nam Hee Kim ◽  
Youn‐Bok Chung ◽  
...  
Keyword(s):  
Human Plasma ◽  
Liquid Extraction ◽  
Hplc Determination ◽  
Liquid Liquid Extraction

Salting-out assisted liquid-liquid extraction method combined with GC-MS for the determination of topiramate in aqueous solutions: development and application of the methodology

2019 ◽  
Vol 55 (13) ◽  
pp. 2303-2312 ◽  
Author(s):  
Janiele Mayara Ferreira De Almeida ◽  
Elania Maria Fernandes Silva ◽  
Lourena Mafra Veríssimo ◽  
Nedja Suely Fernandes
Keyword(s):  
Aqueous Solutions ◽  
Extraction Method ◽  
Liquid Extraction ◽  
Salting Out ◽  
Liquid Liquid Extraction

scholarly journals RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Shravan Bankey ◽  
Ganesh Tapadiya ◽  
Jasvant Lamale ◽  
Deepti Jain ◽  
Shweta Saboo ◽  
...  
Keyword(s):  
Human Plasma ◽  
Mobile Phase ◽  
Method Development ◽  
Bioequivalence Study ◽  
Hplc Method ◽  
Liquid Extraction ◽  
Liquid Liquid Extraction ◽  
Rp Hplc ◽  
Hplc Method Development

A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile : water (90 : 10, v/v), drug was detected at 260 nm using UVdetector. The LOD and LOQ were 3.0 and 10.0 μg/L, respectively. The method is linear in the interval 50.0–1000.0 μg/L. The average extraction recovery of drug from plasma was found to be 92.2%. The percent CV of the method was found to be less than 10.8%, and accuracy was found between 94.5 and 106.7%. The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant.

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