RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma
2012 ◽
Vol 2012
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pp. 1-4
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Keyword(s):
Rp Hplc
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A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile : water (90 : 10, v/v), drug was detected at 260 nm using UVdetector. The LOD and LOQ were 3.0 and 10.0 μg/L, respectively. The method is linear in the interval 50.0–1000.0 μg/L. The average extraction recovery of drug from plasma was found to be 92.2%. The percent CV of the method was found to be less than 10.8%, and accuracy was found between 94.5 and 106.7%. The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant.
2020 ◽
Vol 13
(01)
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2019 ◽
Vol 9
(2)
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2018 ◽
Vol 7
(3)
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