An HPLC Determination of Trimetazidine in Human Plasma Using Liquid‐Liquid Extraction for Sample Clean‐Up

2005 ◽  
Vol 28 (9) ◽  
pp. 1299-1309 ◽  
Author(s):  
Min Kyo Jeoung ◽  
Kyoung Soon Kim ◽  
Chang Soo Kim ◽  
Nam Hee Kim ◽  
Youn‐Bok Chung ◽  
...  
Keyword(s):  
Human Plasma ◽  
Liquid Extraction ◽  
Hplc Determination ◽  
Liquid Liquid Extraction

scholarly journals RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Shravan Bankey ◽  
Ganesh Tapadiya ◽  
Jasvant Lamale ◽  
Deepti Jain ◽  
Shweta Saboo ◽  
...  
Keyword(s):  
Human Plasma ◽  
Mobile Phase ◽  
Method Development ◽  
Bioequivalence Study ◽  
Hplc Method ◽  
Liquid Extraction ◽  
Liquid Liquid Extraction ◽  
Rp Hplc ◽  
Hplc Method Development

A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile : water (90 : 10, v/v), drug was detected at 260 nm using UVdetector. The LOD and LOQ were 3.0 and 10.0 μg/L, respectively. The method is linear in the interval 50.0–1000.0 μg/L. The average extraction recovery of drug from plasma was found to be 92.2%. The percent CV of the method was found to be less than 10.8%, and accuracy was found between 94.5 and 106.7%. The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant.

Optimization of a liquid–liquid extraction method for HPLC–DAD determination of penicillin-V in human plasma

2002 ◽  
Vol 72 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Andrei Medvedovici ◽  
Mihaela Ionescu ◽  
Constantin Mircioiu ◽  
Victor David
Keyword(s):  
Human Plasma ◽  
Extraction Method ◽  
Liquid Extraction ◽  
Penicillin V ◽  
Liquid Liquid Extraction

Validated Liquid–Liquid Extraction and LC–ESI–MS Method for the Determination of Melitracen in Human Plasma

2008 ◽  
Vol 67 (11-12) ◽  
pp. 935-939 ◽  
Author(s):  
Ping Xu ◽  
Huan-De Li ◽  
Yun-Gui Zhu ◽  
Ben-Mei Chen ◽  
Ning Ma ◽  
...  
Keyword(s):  
Human Plasma ◽  
Liquid Extraction ◽  
Esi Ms ◽  
Liquid Liquid Extraction

Determination of doravirine in human plasma using liquid–liquid extraction and HPLC–MS/MS

Bioanalysis ◽  
2019 ◽  
Vol 11 (16) ◽  
pp. 1495-1508
Author(s):  
Rajesh Desai ◽  
Brad Roadcap ◽  
Dina Goykhman ◽  
Eric Woolf
Keyword(s):  
Human Plasma ◽  
Dynamic Range ◽  
Multiple Reaction Monitoring ◽  
Development Program ◽  
Liquid Extraction ◽  
Clinical Development Program ◽  
Liquid Liquid Extraction ◽  
Multiple Reaction Monitoring Mode ◽  
Ionization Mode

Aim: A method to quantitate doravirine (MK-1439) in human plasma has been developed to support human clinical trials designed to evaluate the safety, pharmacokinetics and efficacy of the compound. Methodology & results: The analyte was extracted using liquid–liquid extraction, separated on a reverse phase HPLC column, and detected on an API-4000 mass spectrometer using a Turbo-Ion spray source in positive ionization mode coupled with multiple reaction monitoring mode was used for quantification. The dynamic range for the assay was 0.02–10 ng/ml using 100 μl of human plasma. Conclusion: The assay was found to be sensitive, selective and reproducible and applied to support the doravirine clinical development program.

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