Dibenzothiophene biodegradation by a Pseudomonas sp. in poorly degradable organic solvents

ABSTRACT Extracellular cholesterol oxidase of Pseudomonas sp. strain ST-200 was purified from the culture supernatant. This oxidase contained bound flavin and was categorized as a 3β-hydroxysteroid oxidase, converting 3β-hydroxyl groups to keto groups. The molecular mass was 60 kDa. The enzyme was stable at pH 4 to 11 and active at pH 5.0 to 8.5, showing optimal activity at pH 7 at 60Ã¯Â¿Â½C. The Michaelis constant of the ST-200 cholesterol oxidase was lower than those of commercially available oxidases. The cholesterol oxidation rate was enhanced 3- to 3.5-fold in the presence of organic solvents, with log P ow values (partition coefficients of the organic solvent betweenn-octanol and water), in the range of 2.1 to 4.2, compared with that in the absence of organic solvents.

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New Recombinant Cold-Adapted and Organic Solvent Tolerant Lipase from Psychrophilic Pseudomonas sp. LSK25, Isolated from Signy Island Antarctica

International Journal of Molecular Sciences ◽

10.3390/ijms20061264 ◽

2019 ◽

Vol 20 (6) ◽

pp. 1264 ◽

Cited By ~ 9

Author(s):

Leelatulasi Salwoom ◽

Raja Raja Abd. Rahman ◽

Abu Salleh ◽

Fairolniza Mohd. Shariff ◽

Peter Convey ◽

...

Keyword(s):

Organic Solvent ◽

Organic Solvents ◽

Rice Bran Oil ◽

Lipolytic Activity ◽

Pseudomonas Sp ◽

Lipase Gene ◽

Cold Adapted ◽

Organic Solvent Tolerant ◽

Wide Range ◽

Solvent Tolerant

In recent years, studies on psychrophilic lipases have become an emerging area of research in the field of enzymology. The study described here focuses on the cold-adapted organic solvent tolerant lipase strain Pseudomonas sp. LSK25 isolated from Signy Station, South Orkney Islands, maritime Antarctic. Strain LSK25 lipase was successfully cloned, sequenced, and over-expressed in an Escherichia coli system. Sequence analysis revealed that the lipase gene of Pseudomonas sp. LSK25 consists of 1432 bp, lacks an N-terminal signal peptide and encodes a mature protein consisting of 476 amino acids. The recombinant LSK25 lipase was purified by single-step purification using Ni-Sepharose affinity chromatography and had a molecular mass of approximately 65 kDa. The final recovery and purification fold were 44% and 1.3, respectively. The LSK25 lipase was optimally active at 30 °C and at pH 6. Stable lipolytic activity was reported between temperatures of 5–30 °C and at pH 6–8. A significant enhancement of lipolytic activity was observed in the presence of Ca2+ ions, the organic lipids of rice bran oil and coconut oil, a synthetic C12 ester and a wide range of water immiscible organic solvents. Overall, lipase strain LSK25 is a potentially desirable candidate for biotechnological application, due to its stability at low temperatures, across a range of pH and in organic solvents.

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Characterization of Pseudomonas sp. BCNU 171 tolerant to organic solvents

Journal of Basic Microbiology ◽

10.1002/jobm.200700410 ◽

2008 ◽

Vol 48 (6) ◽

pp. 473-479 ◽

Cited By ~ 3

Author(s):

Hye Jung Choi ◽

Seon-A Kim ◽

Dong Wan Kim ◽

Ja Young Moon ◽

Yong Kee Jeong ◽

...

Keyword(s):

Organic Solvents ◽

Pseudomonas Sp

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Enzymatic dehalogenation of 4-chlorobenzoate by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 in organic solvents

Applied Microbiology and Biotechnology ◽

10.1007/bf00451635 ◽

1988 ◽

Vol 27 (5-6) ◽

pp. 577-580 ◽

Cited By ~ 7

Author(s):

Jürgen Thiele ◽

Rudolf Müller ◽

Franz Lingens

Keyword(s):

Organic Solvents ◽

Pseudomonas Sp

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ChemInform Abstract: Solvent Effect on Pseudomonas sp. Lipase Catalyzed Transesterification Between Tributyrin and 2-Octanol in Organic Solvents.

ChemInform ◽

10.1002/chin.199231076 ◽

2010 ◽

Vol 23 (31) ◽

pp. no-no ◽

Cited By ~ 2

Author(s):

H. HIRATA ◽

K. SAKAKI ◽

T. YAMASHINA ◽

K. HIGUCHI ◽

H. YANAGISHITA ◽

...

Keyword(s):

Solvent Effect ◽

Organic Solvents ◽

Pseudomonas Sp

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342. Effects of Polarity of Mixed Organic Solvents on the Accuracy of Exposure Assessment

10.3320/1.2758375 ◽

2004 ◽

Author(s):

H. Kim ◽

K. Jung

Keyword(s):

Exposure Assessment ◽

Organic Solvents

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UNTERSUCHUNGEN ÜBER DIE EXTRAKTION DES ROTEN KOBER-FARBSTOFFS DURCH ORGANISCHE LÖSUNGSMITTEL ZUR ÖSTROGENBESTIMMUNG IM HARN

Acta Endocrinologica ◽

10.1530/acta.0.xxxv0034 ◽

1960 ◽

Vol XXXV (I) ◽

pp. 34-48 ◽

Cited By ~ 66

Author(s):

Gerd Ittrich

Keyword(s):

Menstrual Cycle ◽

Organic Solvents ◽

Urine Sample ◽

Phenol Derivatives ◽

Organische Lösungsmittel ◽

Colour Complex

ABSTRACT A series of organic solvents and phenol derivatives have been examined for the extraction of the pink Kober-colour complex. Optimal results could be achieved for fluorimetry by a solution of 2 % (w/v) p-nitrophenol and 1 % (v/v) ethanol in acetylenetetrabromide, when the green mercury line (546 mμ was used as primary light. The sensitivity, stability and specificity have been improved, compared with the previously described reaction. By changing the sequence of purification steps and by reducing the volume of the urine sample (5 ml) the method for the determination of total oestrogens has been simplified. Approximately 10 determinations can be done within 3–4 hours by one person. Recovery experiments and comparative determinations with a previously described method have been carried out. The excretion of total oestrogens in a complete menstrual cycle is determined with the described method.

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