Phototrophic biofilms of restored fields in the rhenish lignite mining area: development of soil algal, bacterial, and fungal biomasses

2002 ◽  
Vol 34 (8) ◽  
pp. 1157-1165 ◽  
Author(s):  
J Jahnke ◽  
U.B Priefer
2009 ◽  
Vol 71-73 ◽  
pp. 97-100 ◽  
Author(s):  
H.M. Siebert ◽  
Thore Rohwerder ◽  
Wolfgang Sand ◽  
M. Strzodka ◽  
K.P. Stahmann

The largest lignite mining area in Europe is located 150 km southeast of Berlin. Acidic lakes exist in this area, known to be caused by marcasite oxidation. Thirty-two samples from the open-pit brown coal-mine Jaenschwalde were analyzed for microorganisms. Cell numbers determined after separation from sand particles revealed concentrations of 102 to 107 microorganisms per g sample. In samples exposed to the air within an hour, up to 4x107 cells were counted. Measurement of metabolic activity by microcalorimetry showed for such samples up to 50 µW per g sand, whereas in heap samples (with low moisture) low or even no activity was measurable. DNA extraction was successful for 28 samples. In 26 samples microbial 16S rRNA genes were amplified by PCR. Acidithiobacillus ferrooxidans and At. thiooxidans specific amplificates were detected by nested PCR in 23 and 10 cases, respectively. A specific signal indicating Leptospirillum ferrooxidans was obtained with nine samples. Random samples were sequenced and showed 96 to 99 % identity with published data of all three species. Surprisingly, in four samples archaeal 16S rRNA genes were amplified by PCR. Sequencing of two samples showed 99 % identity with unidentified or uncultured archaea found in NCBI-databases. Molecular biology results for At. ferrooxidans as well as for At. thiooxidans were supported by successful isolations of pure cultures in 23 cases. Cultivation of the archaea failed so far. These data indicate that iron- and sulfur-oxidizing microorganisms occur at these sites in large numbers. If in addition the evidence for archaea can become verified, a screening for hot spots as the sites of their occurrence would become interesting.


2014 ◽  
Vol 30 (3) ◽  
pp. 43-53 ◽  
Author(s):  
Urszula Kazmierczak

Abstract This publication describes a mechanism for shaping the policy of spatial development, the final outputs of which are local land development plans determining the spatial distribution of objects of different environmental and economic functions. Land development plans guarantee a spatial order, which means not only aesthetic qualities, utility, logic, and clarity of spatial structure, but also high usability and effectiveness harmonized with nature, both nationally and locally. Working out such a plan is determined by drawing up many other planning documents. This results from the fact that spatial development should be integrated and comprehensive not only at the local but also at the regional level. What is more, the article presented the results of spatial policy at every stage of deposit management, from a deposit documentation to a post-mining area reclamation, as well as, it discussed issues connected with inadequate protection of the deposits in the planning documents. Documented rock raw material deposits must be included in a study of conditions and directions of commune spatial development (Polish: Studium uwarunkowañ i kierunków zagospodarowania przestrzennego gmin) and in a local land development plan (Miejscowy plan zagospodarowania przestrzennego), and also in voivodeship area development plans. Admittedly, this mention does not protect the deposit from being used for non-mining purposes; still, lack of it might result in failure to obtain the concession for mining of deposits, which is not possible without exclusion of land from the previous use. As for the last phase of mining activity – that is, area reclamation after mining of mineral deposits – such ans area must undergo reclamation and be prepared for development in a way that enables it to function in accordance with the study of conditions and directions of commune spatial development and in the land development plan of the particular commune. This means the necessity of amendments to these documents unless they have already defined target functions of deposit area.


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