Cohesins Bind to Preferential Sites along Yeast Chromosome III, with Differential Regulation along Arms versus the Centric Region

To examine the relationship between genetic and physical chromosome maps, we constructed a diploid strain of the yeast Saccharomyces cerevisiae heterozygous for 12 restriction site mutations within a 23-kilobase (5-centimorgan) interval of chromosome III. Crossovers were not uniformly distributed along the chromosome, one interval containing significantly more and one interval significantly fewer crossovers than expected. One-third of these crossovers occurred within 6 kilobases of the centromere. Approximately half of the exchanges were associated with gene conversion events. The minimum length of gene conversion tracts varied from 4 base pairs to more than 12 kilobases, and these tracts were nonuniformly distributed along the chromosome. We conclude that the chromosomal sequence or structure has a dramatic effect on meiotic recombination.

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The frequency of oligopurine. Oligopyrimidine and other two-base tracts in yeast chromosome III

Yeast ◽

10.1002/yea.320100505 ◽

1994 ◽

Vol 10 (5) ◽

pp. 603-611 ◽

Cited By ~ 10

Author(s):

G. Yagil

Keyword(s):

Yeast Chromosome ◽

Chromosome Iii

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III. Yeast sequencing reports. Nucleotide sequence of 9·2 kb left ofCRY1 on yeast chromosome III from strain ab972: Evidence for a ty insertion and functional analysis of open reading frame YCR28

Yeast ◽

10.1002/yea.320080915 ◽

1992 ◽

Vol 8 (9) ◽

pp. 805-812 ◽

Cited By ~ 6

Author(s):

M. L. Agostoni Carbone ◽

L. Panzeri ◽

M. Muzi Falconi ◽

C. Carcano ◽

P. Plevani ◽

...

Keyword(s):

Functional Analysis ◽

Nucleotide Sequence ◽

Open Reading Frame ◽

Reading Frame ◽

Yeast Chromosome ◽

Chromosome Iii

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Localization of a DNA replication origin and termination zone on chromosome III of Saccharomyces cerevisiae.

Molecular and Cellular Biology ◽

10.1128/mcb.12.10.4733 ◽

1992 ◽

Vol 12 (10) ◽

pp. 4733-4741 ◽

Cited By ~ 35

Author(s):

J Zhu ◽

C S Newlon ◽

J A Huberman

Keyword(s):

Dna Replication ◽

Replication Origin ◽

Replication Forks ◽

Two Dimensional ◽

Yeast Chromosome ◽

Dna Replication Origin ◽

Functional Dna ◽

Mapping Techniques ◽

Dna Replication Origins ◽

Chromosome Iii

Two-dimensional gel electrophoretic replicon mapping techniques were used to identify all functional DNA replication origins and termini in a 26.5-kbp stretch in the left arm of yeast chromosome III. Only one origin was detected; it coincided with an ARS element (ARS306), as have all previously mapped yeast origins. A replication termination region was identified in a 4.3-kbp stretch at the telomere-proximal end of the investigated region, between the origin identified in this paper and the neighboring, previously mapped, ARS305-associated origin (previously called the A6C origin). Termination does not occur at a specific site; instead, it appears to be the consequence of replication forks converging in a stretch of DNA of at least 4.3 kbp.

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Expansions and contractions of the genetic map relative to the physical map of yeast chromosome III.

Molecular and Cellular Biology ◽

10.1128/mcb.8.2.595 ◽

1988 ◽

Vol 8 (2) ◽

pp. 595-604 ◽

Cited By ~ 38

Author(s):

L S Symington ◽

T D Petes

Keyword(s):

Gene Conversion ◽

Restriction Site ◽

Physical Map ◽

Minimum Length ◽

Base Pairs ◽

Yeast Saccharomyces Cerevisiae ◽

Yeast Chromosome ◽

Chromosome Maps ◽

Chromosome Iii ◽

The Relationship

To examine the relationship between genetic and physical chromosome maps, we constructed a diploid strain of the yeast Saccharomyces cerevisiae heterozygous for 12 restriction site mutations within a 23-kilobase (5-centimorgan) interval of chromosome III. Crossovers were not uniformly distributed along the chromosome, one interval containing significantly more and one interval significantly fewer crossovers than expected. One-third of these crossovers occurred within 6 kilobases of the centromere. Approximately half of the exchanges were associated with gene conversion events. The minimum length of gene conversion tracts varied from 4 base pairs to more than 12 kilobases, and these tracts were nonuniformly distributed along the chromosome. We conclude that the chromosomal sequence or structure has a dramatic effect on meiotic recombination.

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