Characterization of the Ehrab8 gene, a marker of the late stages of the secretory pathway of Entamoeba histolytica

2001 ◽  
Vol 116 (2) ◽  
pp. 223-228 ◽  
Author(s):  
Patricia Juárez ◽  
Rosana Sanchez-Lopez ◽  
Roberto P. Stock ◽  
Alejandro Olvera ◽  
Marco A. Ramos ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Secretory Pathway ◽  
Late Stages

scholarly journals Functional Characterization of Spliceosomal Introns and Identification of U2, U4, and U5 snRNAs in the Deep-Branching Eukaryote Entamoeba histolytica

2007 ◽  
Vol 6 (6) ◽  
pp. 940-948 ◽  
Author(s):  
Carrie A. Davis ◽  
Michael P. S. Brown ◽  
Upinder Singh
Keyword(s):  
Entamoeba Histolytica ◽  
Splice Site ◽  
Expressed Sequence Tag ◽  
Functional Characterization ◽  
Molecular Evidence ◽  
Spliceosomal Introns ◽  
Accurate Expression ◽  
Eukaryotic Organisms

ABSTRACT Pre-mRNA splicing is essential to ensure accurate expression of many genes in eukaryotic organisms. In Entamoeba histolytica, a deep-branching eukaryote, approximately 30% of the annotated genes are predicted to contain introns; however, the accuracy of these predictions has not been tested. In this study, we mined an expressed sequence tag (EST) library representing 7% of amoebic genes and found evidence supporting splicing of 60% of the testable intron predictions, the majority of which contain a GUUUGU 5′ splice site and a UAG 3′ splice site. Additionally, we identified several splice site misannotations, evidence for the existence of 30 novel introns in previously annotated genes, and identified novel genes through uncovering their spliced ESTs. Finally, we provided molecular evidence for the E. histolytica U2, U4, and U5 snRNAs. These data lay the foundation for further dissection of the role of RNA processing in E. histolytica gene expression.

Electron probe analysis and biochemical characterization of electron-dense granules secreted by Entamoeba histolytica

1997 ◽  
Vol 85 (2) ◽  
pp. 233-242 ◽  
Author(s):  
Gloria León ◽  
Charles Fiori ◽  
Pradeep Das ◽  
Miguel Moreno ◽  
Rosalinda Tovar ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Electron Probe ◽  
Biochemical Characterization ◽  
Electron Probe Analysis ◽  
Dense Granules ◽  
Probe Analysis

Characterization of a retrotransposon-like element from Entamoeba histolytica

2001 ◽  
Vol 116 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Rachna Sharma ◽  
Anindya Bagchi ◽  
Alok Bhattacharya ◽  
Sudha Bhattacharya
Keyword(s):  
Entamoeba Histolytica

Characterization of N-glycosylations in Entamoeba histolytica ubiquitin

2019 ◽  
Vol 196 ◽  
pp. 38-47
Author(s):  
Adriana Obregón ◽  
María S. Flores ◽  
Roberto Rangel ◽  
Katiuska Arévalo ◽  
Guadalupe Maldonado ◽  
...  
Keyword(s):  
Entamoeba Histolytica

scholarly journals Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates.

1990 ◽  
Vol 58 (10) ◽  
pp. 3458-3461 ◽  
Author(s):  
A Bhattacharya ◽  
R Ghildyal ◽  
S Bhattacharya ◽  
L S Diamond
Keyword(s):  
Monoclonal Antibody ◽  
Entamoeba Histolytica
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