Expression and localization of prostaglandin EP3 receptor in monoaminergic cell groups: A double immunofluorescence study in the rat brain

2000 ◽  
Vol 38 ◽  
pp. S62
Author(s):  
K Nakamura
Keyword(s):  
Rat Brain ◽  
Immunofluorescence Study ◽  
Double Immunofluorescence ◽  
Cell Groups ◽  
Ep3 Receptor

scholarly journals Immunocytochemical staining of the RLM6 form of cytochrome P-450 in oligodendrocytes and myelin of rat brain.

1991 ◽  
Vol 39 (8) ◽  
pp. 1089-1094 ◽  
Author(s):  
W Cammer ◽  
M Downing ◽  
W Clarke ◽  
J B Schenkman
Keyword(s):  
Rat Brain ◽  
Ketone Bodies ◽  
Immunofluorescence Staining ◽  
Immunocytochemical Staining ◽  
Double Immunofluorescence ◽  
Adult Rats ◽  
Adult Rat ◽  
Myelinated Fibers ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Cytochrome P 450

We used immunocytochemical staining to localize the RLM6 form of cytochrome P-450 in rat brain. Immunofluorescence staining in vibratome sections was positive in cells that resembled oligodendrocytes, which are the cells that synthesize and maintain myelin. Double immunofluorescence staining with anti-RLM6, plus mouse monoclonal antibodies (MAb) against 2',3'-cyclic nucleotide-3'-phosphohydrolase or galactocerebrosides, showed localization of each of these oligodendrocyte "markers" in the same cells as RLM6. In vibratome sections from brains of adult rats there was faint RLM6 immunostaining in some of the myelinated fibers as well as in oligodendrocytes. In paraffin sections from adult rat brains, myelinated tracts were RLM6 positive, as were oligodendrocytes and myelinated fibers in the gray matter. Oligodendrocytes were also shown to contain glucose-6-phosphate dehydrogenase. We suggest that RLM6, which is constitutive to liver, is also constitutive to brain and, via the acetone monooxygenase reaction, which also utilizes NADPH, may contribute to the conversion of ketone bodies to substrates that could provide energy for the synthesis and maintenance of myelin.

Neurokinin NK1- and NK3-immunoreactive neurons in serotonergic cell groups in the rat brain

2002 ◽  
Vol 323 (2) ◽  
pp. 146-150 ◽  
Author(s):  
Lucienne Léger ◽  
Nadine Gay ◽  
Raymond Cespuglio
Keyword(s):  
Rat Brain ◽  
Cell Groups ◽  
Serotonergic Cell
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