1827 Carbamazepine inhibits the nociceptive response in the isolated spinal cord

1997 ◽  
Vol 28 ◽  
pp. S226
Author(s):  
Ippei Watanabe ◽  
Makoto Takenoshita
Keyword(s):  
Spinal Cord ◽  
Nociceptive Response

NR2B-siRNA Mediated by Hydroxyapatite Nanoparticles Relieves for Malin-Induced Pain of Mice

2011 ◽  
Vol 343-344 ◽  
pp. 926-932 ◽  
Author(s):  
Yong Hong Gu ◽  
Xue Bin Yan ◽  
Dong Huang ◽  
Rui Han ◽  
Li Xiang Wu
Keyword(s):  
Spinal Cord ◽  
Immunohistochemical Staining ◽  
Formalin Test ◽  
Fluorescent Protein ◽  
Intrathecal Injection ◽  
Nociceptive Response ◽  
Hydroxyapatite Nanoparticles ◽  
Green Fluorescent ◽  
Tonic Phase

To observe the effect of NR2B-siRNA mediated by hydroxyapatite nanoparticles (HA) on formalin-induced inflammatory pain of mice and the expression of NR2B in spinal cord. To preliminarily investigate the feasibility of HA as siRNA carrier to transfer NR2B-siRNA in vivo. The sequence-specific NR2B-siRNA of mice was designed and synthesized initially. Using HA as a siRNA carrier, green fluorescent protein(GFP)-siRNA as the control, 4 ug of NR2B-siRNA was administered into subarachnoid space of mice via conscious injection. On 7th day after intrathecal injection, formalin test was observed for 1 hour in each group, followed by dissection of lumbar segments of spinal cords immediately for use in immunohistochemical staining of NR2B. The results show that NR2B-siRNA not only significantly abolish the nociceptive response of mice in the tonic phase induced by formalin, but also decrease the amount of cells expressing NR2B protein in spinal cord, while GFP-siRNA mediated by HA don’t produce the same effects, which demonstrates that HA is capable of effectively transfering NR2B-siRNA via intrathecal injection, furthermore, HA/NR2B-siRNA complex can significantly reduce formalin-induced pain of mice, and specificly inhibit NR2B expression in spinal cord of mice.

Hyperthyroidism changes nociceptive response and ecto-nucleotidase activities in synaptosomes from spinal cord of rats in different phases of development

2005 ◽  
Vol 140 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Alessandra Nejar Bruno ◽  
Fernanda Urruth Fontella ◽  
Leonardo Machado Crema ◽  
Carla Denise Bonan ◽  
Carla Dalmaz ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Nociceptive Response ◽  
Phases Of Development

scholarly journals Puerarin Alleviates Neuropathic Pain by Inhibiting Neuroinflammation in Spinal Cord

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Ming Liu ◽  
Kaijun Liao ◽  
Changxi Yu ◽  
Xuejun Li ◽  
Suhuan Liu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Neuropathic Pain ◽  
Spinal Dorsal Horn ◽  
Chronic Constriction Injury ◽  
Intrathecal Administration ◽  
Nociceptive Response ◽  
Nuclear Factor ◽  
Spinal Dorsal ◽  
Drug Treatments ◽  
Anti Inflammation

Neuropathic pain responds poorly to drug treatments, and partial relief is achieved in only about half of the patients. Puerarin, the main constituent ofPuerariae Lobatae Radix, has been used extensively in China to treat hypertension and tumor. The current study examined the effects of puerarin on neuropathic pain using two most commonly used animal models: chronic constriction injury (CCI) and diabetic neuropathy. We found that consecutive intrathecal administration of puerarin (4–100 nM) for 7 days inhibited the mechanical and thermal nociceptive response induced by CCI and diabetes without interfering with the normal pain response. Meanwhile, in both models puerarin inhibited the activation of microglia and astroglia in the spinal dorsal horn. Puerarin also reduced the upregulated levels of nuclear factor-κB (NF-κB) and other proinflammatory cytokines, such as IL-6, IL-1β, and TNF-α, in the spinal cord. In summary, puerarin alleviated CCI- and diabetes-induced neuropathic pain, and its effectiveness might be due to the inhibition of neuroinflammation in the spinal cord. The anti-inflammation effect of puerarin might be related to the suppression of spinal NF-κB activation and/or cytokines upregulation. We conclude that puerarin has a significant effect on alleviating neuropathic pain and thus may serve as a therapeutic approach for neuropathic pain.

Nociceptive Response and Adenine Nucleotide Hydrolysis in Synaptosomes Isolated from Spinal Cord of Hypothyroid Rats

2005 ◽  
Vol 30 (9) ◽  
pp. 1155-1161 ◽  
Author(s):  
Alessandra Nejar Bruno ◽  
Daniela Pochmann ◽  
Felipe Klein Ricachenevsky ◽  
Fernanda Urruth Fontella ◽  
Carla Denise Bonan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Adenine Nucleotide ◽  
Nociceptive Response ◽  
Nucleotide Hydrolysis

SITES AT WHICH FORMALIN AND CAPSAICIN ACT TO STIMULATE CORTICOTROPIN SECRETION

1967 ◽  
Vol 45 (4) ◽  
pp. 669-674 ◽  
Author(s):  
G. B. Makara ◽  
E. Stark ◽  
K. Mihály
Keyword(s):  
Spinal Cord ◽  
Corticosterone Level ◽  
Plasma Corticosterone ◽  
Nociceptive Response ◽  
Plasma Corticosterone Level ◽  
Acth Secretion ◽  
Mucous Membranes ◽  
Ascending Pathways ◽  
Male Wistar Rats ◽  
Stimulation Of

The sites at which injected Formalin and capsaicin act to stimulate ACTH secretion, as concluded from changes in the plasma corticosterone level, were studied in male Wistar rats. Formalin injected into an area deafferentated by denervation or spinal cord transection failed to raise the plasma corticosterone level, showing that it acted locally and that the nervous impulses it produced travelled to the hypothalamus through ascending pathways in the spinal cord. Capsaicin proved to be a potent stressor agent, and it produced a rise in the corticosterone level even when injected into a denervated area. This shows that chemonociceptor excitation at the site of injection is only one of several factors responsible for the stimulation of ACTH secretion by capsaicin. Pretreatment with capsaicin "desensitizing" the chemonociceptors in the skin and the mucous membranes merely inhibited, but did not suppress, the rise in the corticosterone level produced by a subsequent injection of capsaicin; it had no effect on the "nociceptive response" and the rise in the corticosterone level elicited by Formalin.

Alpha-2B adrenoceptor activation inhibits a nociceptive response in the spinal cord of the neonatal rat

1990 ◽  
Vol 183 (3) ◽  
pp. 740 ◽  
Author(s):  
M.K.T. Savola ◽  
S.J. Woodley ◽  
J.J. Kendig ◽  
M. Maze
Keyword(s):  
Spinal Cord ◽  
Neonatal Rat ◽  
Nociceptive Response
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