Distribution, abundance and seasonality of scale insects in sugarcane crops in the state of São Paulo

In the state of São Paulo, the main sugarcane producing region of the world, two species of scale insects have frequently occurred, Aclerda takahashii (Kuwana, 1932) (Hemiptera: Aclerdidae) and Saccharicoccus sacchari (Cockerell, 1895) (Hemiptera: Pseudococcidae). To map the distribution and abundance of these species, 17 sugarcane producing fields, distributed in six mesoregions in São Paulo, were evaluated in August 2017 and, January, February, June and July 2018 during the ripening phase. The study on the seasonality of these species, by the presence or absence of the scale insects during the phenological cycle of the plant, was conducted between August 2017 and July 2018 in two sugarcane producing fields in the municipality of Jaboticabal, São Paulo, Brazil. The presence of S. sacchari was found in all the analyzed locations, and A. takahashii in twelve. Both scale insects showed significant difference of infestation in the node’s region of the stems during the ripening phase in one of the studied locations. The aclerdid presented significant difference by infestation in one site during the ripening phase of the plant. The pseudococcid infested a greater number of nodes in the following phases of development; vegetative, grand growth and ripening in both studied areas, but it was in one site during the ripening phase that presented the greatest difference. Although the pink sugarcane mealybug was more abundant than A. takahashii in both studies, there were no patterns of relationships between the numbers of individuals to geographical locations and temperature.

Presence of an alien turtle accelerates hatching of common frog (Rana temporaria) tadpoles

The presence of a predator affects prey populations either by direct predation or by modifying various parts of their life history. We investigated whether the hatching time, developmental stage, and body size at hatching of common frog (Rana temporaria) embryos would be altered in the presence of a red-eared slider (Trachemys scripta elegans) as a predator. The presence of a predator affected all factors examined. We found that in the absence of the slider, the embryos hatched in 12 days, while hatching was accelerated by two days when sliders were present. At the same time, the embryos hatched smaller and at a lower stage of development with the slider than without it. Our study extends the range of predators studied, including the effect on different phases of development of potential amphibian prey.

Novel antifungal agents in clinical trials

Invasive fungal diseases due to resistant yeasts and molds are an important and increasing public health threat, likely due to a growing population of immunosuppressed hosts, increases in antifungal resistance, and improvements in laboratory diagnostics. The significant morbidity and mortality associated with these pathogens bespeaks the urgent need for novel safe and effective therapeutics. This review highlights promising investigational antifungal agents in clinical phases of development: fosmanogepix, ibrexafungerp, rezafungin, encochleated amphotericin B, oteseconazole (VT-1161), VT-1598, PC945, and olorofim. We discuss three first-in-class members of three novel antifungal classes, as well as new agents within existing antifungal classes with improved safety and tolerability profiles due to enhanced pharmacokinetic and pharmacodynamic properties.

SCF Ligases and Their Functions in Oogenesis and Embryogenesis—Summary of the Most Important Findings throughout the Animal Kingdom

SCF-dependent proteolysis was first discovered via genetic screening of budding yeast almost 25 years ago. In recent years, more and more functions of SCF (Skp1-Cullin 1-F-box) ligases have been described, and we can expect the number of studies on this topic to increase. SCF ligases, which are E3 ubiquitin multi-protein enzymes, catalyse protein ubiquitination and thus allow protein degradation mediated by the 26S proteasome. They play a crucial role in the degradation of cell cycle regulators, regulation of the DNA repair and centrosome cycle and play an important role in several diseases. SCF ligases seem to be needed during all phases of development, from oocyte formation through fertilization, activation of the embryonic genome to embryo implantation. In this review, we summarize known data on SCF ligase-mediated degradation during oogenesis and embryogenesis. In particular, SCFβTrCP and SCFSEL-10/FBXW7 are among the most important and best researched ligases during early development. SCFβTrCP is crucial for the oogenesis of Xenopus and mouse and also in Xenopus and Drosophila embryogenesis. SCFSEL-10/FBXW7 participates in the degradation of several RNA-binding proteins and thereby affects the regulation of gene expression during the meiosis of C. elegans. Nevertheless, a large number of SCF ligases that are primarily involved in embryogenesis remain to be elucidated.

Tentacle patterning during Exaiptasia diaphana pedal lacerate development differs between symbiotic and aposymbiotic animals

Exaiptasia diaphana, a tropical sea anemone known as Aiptasia, is a tractable model system for studying the cellular, physiological, and ecological characteristics of cnidarian-dinoflagellate symbiosis. Aiptasia is widely used as a proxy for coral-algal symbiosis, since both Aiptasia and corals form a symbiosis with members of the family Symbiodiniaceae. Laboratory strains of Aiptasia can be maintained in both the symbiotic (Sym) and aposymbiotic (Apo, without algae) states. Apo Aiptasia allow for the study of the influence of symbiosis on different biological processes and how different environmental conditions impact symbiosis. A key feature of Aiptasia is the ease of propagating both Sym and Apo individuals in the laboratory through a process called pedal laceration. In this form of asexual reproduction, small pieces of tissue rip away from the pedal disc of a polyp, then these lacerates eventually develop tentacles and grow into new polyps. While pedal laceration has been described in the past, details of how tentacles are formed or how symbiotic and nutritional state influence this process are lacking. Here we describe the stages of development in both Sym and Apo pedal lacerates. Our results show that Apo lacerates develop tentacles earlier than Sym lacerates, while over the course of 20 days, Sym lacerates end up with a greater number of tentacles. We describe both tentacle and mesentery patterning during lacerate development and show that they form through a single pattern in early stages regardless of symbiotic state. In later stages of development, Apo lacerate tentacles and mesenteries progress through a single pattern, while variable patterns were observed in Sym lacerates. We discuss how Aiptasia lacerate mesentery and tentacle patterning differs from oral disc regeneration and how these patterning events compare to postembryonic development in Nematostella vectensis, another widely-used sea anemone model. In addition, we demonstrate that Apo lacerates supplemented with a putative nutrient source developed an intermediate number of tentacles between un-fed Apo and Sym lacerates. Based on these observations, we hypothesize that pedal lacerates progress through two different, putatively nutrient-dependent phases of development. In the early phase, the lacerate, regardless of symbiotic state, preferentially uses or relies on nutrients carried over from the adult polyp. These resources are sufficient for lacerates to develop into a functional polyp. In the late phase of development, continued growth and tentacle formation is supported by nutrients obtained from either symbionts and/or the environment through heterotrophic feeding. Finally, we advocate for the implementation of pedal lacerates as an additional resource in the Aiptasia model system toolkit for studies of cnidarian-dinoflagellate symbiosis.

Platelet-rich plasma injection for tennis elbow: did it ever work?

Platelet-rich plasma (PRP) is a commonly used treatment for tendinopathies such as tennis elbow despite the questionable evidence of its efficacy. A recent Cochrane review suggests that it likely does not provide clinically meaningful benefits in people with tennis elbow. In this viewpoint, we discuss how lack of regulation allowed aggressive marketing and clinical use without normal phases of drug development and approval process or rigorous evidence of benefits. Since several phases of development were bypassed, we still do not know the optimal preparation method and dosing of PRP for tendinopathies. Furthermore, several clinical trials compared PRP with other interventions although it was unclear if PRP was better than placebo and these comparisons created distraction rather than improved understanding of its effects.

DEPENDENCE OF SPRING WHEAT YIELD ON HYDROTHERMIC CONDITIONS IN THE SARATOV REGION

Using long-term stationary field studies on the phases of development of spring wheat, a prognostic equation for calculating the yield of a given crop depending on the duration of the growing season with a monthly lead time was derived. Taking into account the hydrothermic factors of the Saratov region, the prognostic equation of the multiple function of the yield of spring wheat from the amount of precipitation for April-May, the average air temperature in May, the amount of precipitation in June, the amount of precipitation in July, and the duration of the growing season was calculated.

Development of an Enrichment Book of Colloid Industry Products as a Source of Literacy for Senior High School Students

The aim of this research was to develop an enrichment book of colloid industry products as a source of literacy and to determine the quality of this book. The approach used in this study was Design-Based Research, which included four phases of development: 1) a questionnaire about needs was filled in by 5 teachers and 83 students; 2) the book was developed using CorelDraw X8 software and a literature search was conducted of online articles, journals and other supporting books; 3) cycles of testing were conducted, in which three experts and three peer reviewers assessed the book, and several revisions were produced; and 4) reflection and final evaluation were conducted, in which 5 teachers and 20 students from SMA N 2 Sleman, SMA N 2 Yogyakarta, SMA 1 Tempel, and MAN 3 Sleman assessed the substance, presentation, language and graphics of the book. Through this process, an enrichment book of colloidal industry products titled “Proses Pembuatan Koloid di Industri untuk SMA/MA Kelas XI” was produced. The book received a score 62.8 out of a maximum score of 70, which corresponded to a rating of ‘very good quality’. Keywords: colloid, literacy, enrichment book

Socio-Psychological Mechanisms of Entrepreneurial Activity in the Youth Environment

Entrepreneurship in Russia and the Chuvash Republic is undergoing new phases of development, a new generation of entrepreneurs has appeared, the one that formed under contemporary socio-economic conditions. The article examines the results of the undertaken empiric research on psychological peculiarities of young entrepreneurs, socio-psychological mechanisms of entrepreneurial activity in modern environment, economic and psychological as well as socio-psychological phenomena in modern entrepreneurship. Features of value sphere, subjective economic wealth, professional types of personality, socio-psychological setup of young entrepreneurs, engaging in entrepreneurial activity in the Chuvash Republic and Russia.

LEADOR: A Method for End-To-End Participatory Design of Autonomous Social Robots

Participatory design (PD) has been used to good success in human-robot interaction (HRI) but typically remains limited to the early phases of development, with subsequent robot behaviours then being hardcoded by engineers or utilised in Wizard-of-Oz (WoZ) systems that rarely achieve autonomy. In this article, we present LEADOR (Led-by-Experts Automation and Design Of Robots), an end-to-end PD methodology for domain expert co-design, automation, and evaluation of social robot behaviour. This method starts with typical PD, working with the domain expert(s) to co-design the interaction specifications and state and action space of the robot. It then replaces the traditional offline programming or WoZ phase by an in situ and online teaching phase where the domain expert can live-program or teach the robot how to behave whilst being embedded in the interaction context. We point out that this live teaching phase can be best achieved by adding a learning component to a WoZ setup, which captures implicit knowledge of experts, as they intuitively respond to the dynamics of the situation. The robot then progressively learns an appropriate, expert-approved policy, ultimately leading to full autonomy, even in sensitive and/or ill-defined environments. However, LEADOR is agnostic to the exact technical approach used to facilitate this learning process. The extensive inclusion of the domain expert(s) in robot design represents established responsible innovation practice, lending credibility to the system both during the teaching phase and when operating autonomously. The combination of this expert inclusion with the focus on in situ development also means that LEADOR supports a mutual shaping approach to social robotics. We draw on two previously published, foundational works from which this (generalisable) methodology has been derived to demonstrate the feasibility and worth of this approach, provide concrete examples in its application, and identify limitations and opportunities when applying this framework in new environments.