Nociceptive Response and Adenine Nucleotide Hydrolysis in Synaptosomes Isolated from Spinal Cord of Hypothyroid Rats

To observe the effect of NR2B-siRNA mediated by hydroxyapatite nanoparticles (HA) on formalin-induced inflammatory pain of mice and the expression of NR2B in spinal cord. To preliminarily investigate the feasibility of HA as siRNA carrier to transfer NR2B-siRNA in vivo. The sequence-specific NR2B-siRNA of mice was designed and synthesized initially. Using HA as a siRNA carrier, green fluorescent protein(GFP)-siRNA as the control, 4 ug of NR2B-siRNA was administered into subarachnoid space of mice via conscious injection. On 7th day after intrathecal injection, formalin test was observed for 1 hour in each group, followed by dissection of lumbar segments of spinal cords immediately for use in immunohistochemical staining of NR2B. The results show that NR2B-siRNA not only significantly abolish the nociceptive response of mice in the tonic phase induced by formalin, but also decrease the amount of cells expressing NR2B protein in spinal cord, while GFP-siRNA mediated by HA don’t produce the same effects, which demonstrates that HA is capable of effectively transfering NR2B-siRNA via intrathecal injection, furthermore, HA/NR2B-siRNA complex can significantly reduce formalin-induced pain of mice, and specificly inhibit NR2B expression in spinal cord of mice.

Download Full-text

The effect of ebselen on adenine nucleotide hydrolysis by platelets from adult rats

Chemico-Biological Interactions ◽

10.1016/j.cbi.2004.04.003 ◽

2004 ◽

Vol 148 (1-2) ◽

pp. 93-99 ◽

Cited By ~ 16

Author(s):

Cristina Ribas Fürstenau ◽

Ana Paula Spier ◽

Bárbara Rücker ◽

Simone Luisa Berti ◽

Ana Maria Oliveira Battastini ◽

...

Keyword(s):

Adenine Nucleotide ◽

Adult Rats ◽

Nucleotide Hydrolysis

Download Full-text

Decrease of serum adenine nucleotide hydrolysis in an irritant contact dermatitis mice model: potential P2X7R involvement

Molecular and Cellular Biochemistry ◽

10.1007/s11010-015-2381-7 ◽

2015 ◽

Vol 404 (1-2) ◽

pp. 221-228 ◽

Cited By ~ 4

Author(s):

R. F. Zanin ◽

G. L. da Silva ◽

T. Erig ◽

N. D. M. Sperotto ◽

C. E. Leite ◽

...

Keyword(s):

Contact Dermatitis ◽

Adenine Nucleotide ◽

Model Potential ◽

Irritant Contact Dermatitis ◽

Mice Model ◽

Nucleotide Hydrolysis

Download Full-text

1827 Carbamazepine inhibits the nociceptive response in the isolated spinal cord

Neuroscience Research ◽

10.1016/s0168-0102(97)90617-9 ◽

1997 ◽

Vol 28 ◽

pp. S226

Author(s):

Ippei Watanabe ◽

Makoto Takenoshita

Keyword(s):

Spinal Cord ◽

Nociceptive Response

Download Full-text

Intracerebral microinjections of cyclazocine inhibit a nociceptive response of the lamina V-type neuron of the rabbit spinal cord

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)54386-4 ◽

1981 ◽

Vol 31 ◽

pp. 65

Author(s):

Shinichi Kawajiri ◽

Masamichi Satoh ◽

Hiroshi Takaqi

Keyword(s):

Spinal Cord ◽

Nociceptive Response

Download Full-text

Hyperthyroidism changes nociceptive response and ecto-nucleotidase activities in synaptosomes from spinal cord of rats in different phases of development

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology ◽

10.1016/j.cbpb.2004.11.007 ◽

2005 ◽

Vol 140 (1) ◽

pp. 111-116 ◽

Cited By ~ 9

Author(s):

Alessandra Nejar Bruno ◽

Fernanda Urruth Fontella ◽

Leonardo Machado Crema ◽

Carla Denise Bonan ◽

Carla Dalmaz ◽

...

Keyword(s):

Spinal Cord ◽

Nociceptive Response ◽

Phases Of Development

Download Full-text

Puerarin Alleviates Neuropathic Pain by Inhibiting Neuroinflammation in Spinal Cord

Mediators of Inflammation ◽

10.1155/2014/485927 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 14

Author(s):

Ming Liu ◽

Kaijun Liao ◽

Changxi Yu ◽

Xuejun Li ◽

Suhuan Liu ◽

...

Keyword(s):

Spinal Cord ◽

Neuropathic Pain ◽

Spinal Dorsal Horn ◽

Chronic Constriction Injury ◽

Intrathecal Administration ◽

Nociceptive Response ◽

Nuclear Factor ◽

Spinal Dorsal ◽

Drug Treatments ◽

Anti Inflammation

Neuropathic pain responds poorly to drug treatments, and partial relief is achieved in only about half of the patients. Puerarin, the main constituent ofPuerariae Lobatae Radix, has been used extensively in China to treat hypertension and tumor. The current study examined the effects of puerarin on neuropathic pain using two most commonly used animal models: chronic constriction injury (CCI) and diabetic neuropathy. We found that consecutive intrathecal administration of puerarin (4–100 nM) for 7 days inhibited the mechanical and thermal nociceptive response induced by CCI and diabetes without interfering with the normal pain response. Meanwhile, in both models puerarin inhibited the activation of microglia and astroglia in the spinal dorsal horn. Puerarin also reduced the upregulated levels of nuclear factor-κB (NF-κB) and other proinflammatory cytokines, such as IL-6, IL-1β, and TNF-α, in the spinal cord. In summary, puerarin alleviated CCI- and diabetes-induced neuropathic pain, and its effectiveness might be due to the inhibition of neuroinflammation in the spinal cord. The anti-inflammation effect of puerarin might be related to the suppression of spinal NF-κB activation and/or cytokines upregulation. We conclude that puerarin has a significant effect on alleviating neuropathic pain and thus may serve as a therapeutic approach for neuropathic pain.

Download Full-text