Effects of replacing the movement protein gene of Tobacco mosaic virus by that of Tomato mosaic virus

A novel genetic screen was used to identify host factors in Arabidopsis thaliana that suppress mutations in the Cauliflower mosaic virus (CaMV) movement protein gene (gene I). A series of small mutations was made in gene I and the mutations were tested for their suitability in a suppressor screen. The first round of screening yielded only revertants or second-site mutations in gene I. A derivative of one of the second-site mutant viruses (N7) that was delayed in symptom production was used in a second round of screening for suppressor plants that accelerated symptom production. Two candidate suppressor plants were found that accelerated by 1 to 4 days the first appearance of symptoms caused by the mutant viruses. One of the suppressors (5-2), called asc1 (acceleration of symptoms by CaMV N7), was mapped to chromosome 1. Two additional loci that differentially affect N7 virus susceptibility in the parental Columbia and Ler ecotypes were mapped to chromosomes 3 and 4 by quantitative trait locus (QTL) analysis.

Download Full-text

Analysis of local and systemic spread of the crucifer-infecting TMV-Cg virus in tobacco and several Arabidopsis thaliana ecotypes

Functional Plant Biology ◽

10.1071/fp02145 ◽

2003 ◽

Vol 30 (4) ◽

pp. 401 ◽

Cited By ~ 9

Author(s):

Patricio Arce-Johnson ◽

Consuelo Medina ◽

Hal S. Padgett ◽

Wilson Huanca ◽

Carmen Espinoza

Keyword(s):

Arabidopsis Thaliana ◽

Tobacco Mosaic Virus ◽

Mosaic Virus ◽

Movement Protein ◽

Host Interactions ◽

Nicotiana Tabacum L ◽

Systemic Spread ◽

Movement Protein Gene ◽

Local Movement

The crucifer-infecting tobacco mosaic virus, TMV-Cg, infects Arabidopsis thaliana (L.) Heynh. efficiently without causing severe symptoms. The systemic spread of TMV-Cg in Arabidopsis was evaluated in 14�ecotypes. Five days after inoculation, TMV-Cg was detected in apical leaves of 8 out of 14 ecotypes. As expected, the spread of TMV-Cg in the ecotypes tested was considerably faster than that of tobacco mosaic virus (TMV-U1). To study the participation of viral proteins in the TMV-Cg-induced infection, a complete genomic cDNA of TMV-Cg was cloned. The role of TMV-Cg movement protein in systemic spread was tested with a hybrid virus, constructed from the TMV-U1 genome and the TMV-Cg movement protein gene. Contrary to expectations, the systemic spread of this hybrid in Arabidopsis was similar to that of TMV-U1. The failure of the hybrid virus to spread at rates similar to those of TMV-Cg was not due to restrictions in local movement. In tobacco (Nicotiana tabacum L.), the hybrid virus spread efficiently and induced systemic mosaic symptoms characteristic of TMV-U1. The TMV-Cg cDNA clone provides an attractive tool to study virus–host interactions.

Download Full-text

Identification of a movement protein of Mirafiori lettuce big-vein ophiovirus

Journal of General Virology ◽

10.1099/vir.0.050005-0 ◽

2013 ◽

Vol 94 (5) ◽

pp. 1145-1150 ◽

Cited By ~ 5

Author(s):

Akihiro Hiraguri ◽

Shoko Ueki ◽

Hideki Kondo ◽

Koji Nomiyama ◽

Takumi Shimizu ◽

...

Keyword(s):

Mosaic Virus ◽

Nicotiana Benthamiana ◽

Protein Gene ◽

Movement Protein ◽

Rna Virus ◽

Infectious Clone ◽

Microprojectile Bombardment ◽

Tomato Mosaic Virus ◽

Intercellular Movement ◽

Heterologous Virus

Mirafiori lettuce big-vein virus (MiLBVV) is a member of the genus Ophiovirus, which is a segmented negative-stranded RNA virus. In microprojectile bombardment experiments to identify a movement protein (MP) gene of ophioviruses that can trans-complement intercellular movement of an MP-deficient heterologous virus, a plasmid containing an infectious clone of a tomato mosaic virus (ToMV) derivative expressing the GFP was co-bombarded with plasmids containing one of three genes from MiLBVV RNAs 1, 2 and 4 onto Nicotiana benthamiana. Intercellular movement of the movement-defective ToMV was restored by co-expression of the 55 kDa protein gene, but not with the two other genes. Transient expression in epidermal cells of N. benthamiana and onion showed that the 55 kDa protein with GFP was localized on the plasmodesmata. The 55 kDa protein encoded in the MiLBVV RNA2 can function as an MP of the virus. This report is the first to describe an ophiovirus MP.

Download Full-text