P0691 : Knockdown of lysophosphatidylcholine acyltransferase 1 (LPCAT1) increases both the secretion of very-low-density lipoproteins (VLDL) and the infectivity of hepatitis C virus (HCV)

2015 ◽  
Vol 62 ◽  
pp. S581
Author(s):  
M. Lemasson ◽  
F. Beilstein ◽  
V. Pène ◽  
A.R. Rosenberg ◽  
S. Demignot
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins

scholarly journals Cellular Determinants of Hepatitis C Virus Assembly, Maturation, Degradation, and Secretion

2007 ◽  
Vol 82 (5) ◽  
pp. 2120-2129 ◽  
Author(s):  
Pablo Gastaminza ◽  
Guofeng Cheng ◽  
Stefan Wieland ◽  
Jin Zhong ◽  
Wei Liao ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Assembly ◽  
Buoyant Density ◽  
Infectious Hepatitis ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Independent Manner ◽  
Viral Egress ◽  
Vldl Assembly

ABSTRACT Intracellular infectious hepatitis C virus (HCV) particles display a distinctly higher buoyant density than do secreted virus particles, suggesting that the characteristic low density of extracellular HCV particles is acquired during viral egress. We took advantage of this difference to examine the determinants of assembly, maturation, degradation, and egress of infectious HCV particles. The results demonstrate that HCV assembly and maturation occur in the endoplasmic reticulum (ER) and post-ER compartments, respectively, and that both depend on microsomal transfer protein and apolipoprotein B, in a manner that parallels the formation of very-low-density lipoproteins (VLDL). In addition, they illustrate that only low-density particles are efficiently secreted and that immature particles are actively degraded, in a proteasome-independent manner, in a post-ER compartment of the cell. These results suggest that by coopting the VLDL assembly, maturation, degradation, and secretory machinery of the cell, HCV acquires its hepatocyte tropism and, by mimicry, its tendency to persist.

scholarly journals Hepatitis C virus cell entry: role of lipoproteins and cellular receptors

2009 ◽  
Vol 90 (5) ◽  
pp. 1055-1070 ◽  
Author(s):  
Michela E. Burlone ◽  
Agata Budkowska
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Current Knowledge ◽  
Scavenger Receptor ◽  
Cell Entry ◽  
Host Cells ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Junction Molecules

Hepatitis C virus (HCV), a major cause of chronic liver disease, is a single-stranded positive sense virus of the family Flaviviridae. HCV cell entry is a multi-step process, involving several viral and cellular factors that trigger virus uptake into the hepatocyte. Tetraspanin CD81, human scavenger receptor SR-BI, and tight junction molecules Claudin-1 and occludin are the main receptors that mediate HCV entry. In addition, the virus may use glycosaminoglycans and/or low density receptors on host cells as initial attachment factors. A unique feature of HCV is the dependence of virus replication and assembly on host cell lipid metabolism. Most notably, during HCV assembly and release from the infected cells, virus particles associate with lipids and very-low-density lipoproteins. Thus, infectious virus circulates in patient sera in the form of triglyceride-rich particles. Consequently, lipoproteins and lipoprotein receptors play an essential role in virus uptake and the initiation of infection. This review summarizes the current knowledge about HCV receptors, mechanisms of HCV cell entry and the role of lipoproteins in this process.

Hepatitis C virus production requires apolipoprotein A-I and affects its association with nascent low-density lipoproteins

Gut ◽  
2010 ◽  
Vol 60 (3) ◽  
pp. 378-386 ◽  
Author(s):  
C. Mancone ◽  
C. Steindler ◽  
L. Santangelo ◽  
G. Simonte ◽  
C. Vlassi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Production ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Apolipoprotein A

scholarly journals Studies of the Proteins in Human Plasma Very Low Density Lipoproteins

1969 ◽  
Vol 244 (20) ◽  
pp. 5687-5694 ◽  
Author(s):  
W. Virgil Brown ◽  
Robert I. Levy ◽  
Donald S. Fredrickson
Keyword(s):  
Human Plasma ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins
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