Characterization of the effect of lipooxygenase arachidonic acid pathway (LO) Inhibitors on bile salt excretion by isolated rat hepatocytes

1985 ◽  
Vol 1 ◽  
pp. S120
Keyword(s):  
Arachidonic Acid ◽  
Bile Salt ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Salt Excretion ◽  
Acid Pathway ◽  
Arachidonic Acid Pathway ◽  
Isolated Rat

Inhibitors of the lipoxygenase arachidonic acid pathway impair glycocholate efflux in isolated rat hepatocytes

1991 ◽  
Vol 12 (3) ◽  
pp. 302-311 ◽  
Author(s):  
Jorge Quiroga ◽  
JoséL. Rodríguez-Sanromán ◽  
Francisco Guarner ◽  
Carlos Rodríguez Ortigosa ◽  
JoséM. Aréjola ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Acid Pathway ◽  
Arachidonic Acid Pathway ◽  
Isolated Rat

Characterization of the Binding of Bovine Thrombin to Isolated Rat Hepatocytes

1988 ◽  
Vol 60 (03) ◽  
pp. 419-427 ◽  
Author(s):  
Britta Weyer ◽  
Torben E Petersen ◽  
Ole Sonne
Keyword(s):  
Binding Sites ◽  
Time Course ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Prolonged Incubation ◽  
Receptor Assay ◽  
Isolated Rat

SummaryIsolated rat hepatocytes possess per cell 4,500 high-affinity binding sites for thrombin with a Kd of 30-40 pM, and 2.8 × 105 low-affinity sites with a Kd of 30 nM. These binding sites are highly specific for thrombin. Half-maximal binding of 125I-labelled thrombin is achieved after 3 min at 37¸ C and 7 min at 4¸ C. The reversibly bound fraction of the ligand dissociates according to a biexponential time course with the rate constants 1—2 × 10−2 s−1 and 3—4 × 10−4 s−1. Part of the tracer remains cell-associated even after prolonged incubation, but all cell-associated radioactivity migrates as intact thrombin upon sodium dodecyl sulphate polyacrylamide gel electrophoresis. The bound thrombin is minimally endocytosed as judged by the resistance to pH 3-treatment. Cell-associated radioactivity dissociated from the cells binds just aswell in a receptor assay as tracer incubated in a conditioned medium under the same conditions, indicating the absence of a quantitatively important receptor-mediated degradation ofthe ligand.

Characterization of Somatogenic and Lactogenic Binding Sites in Isolated Rat Hepatocytes

Endocrinology ◽  
1976 ◽  
Vol 99 (4) ◽  
pp. 1033-1045 ◽  
Author(s):  
MICHAEL B. RANKE ◽  
CHARLES A. STANLEY ◽  
ALFRED TENORE ◽  
DAVID RODBARD ◽  
ALFRED M. BONGIOVANNI ◽  
...  
Keyword(s):  
Binding Sites ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat
Sign in / Sign up

Export Citation Format

Share Document