scholarly journals Lipoprotein secretion by isolated rat hepatocytes: characterization of the lipid-carrying particles and modulation of their release.

1980 ◽  
Vol 21 (6) ◽  
pp. 671-680
Author(s):  
H J Kempen
Keyword(s):  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Lipoprotein Secretion ◽  
Isolated Rat

Characterization of the Binding of Bovine Thrombin to Isolated Rat Hepatocytes

1988 ◽  
Vol 60 (03) ◽  
pp. 419-427 ◽  
Author(s):  
Britta Weyer ◽  
Torben E Petersen ◽  
Ole Sonne
Keyword(s):  
Binding Sites ◽  
Time Course ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Prolonged Incubation ◽  
Receptor Assay ◽  
Isolated Rat

SummaryIsolated rat hepatocytes possess per cell 4,500 high-affinity binding sites for thrombin with a Kd of 30-40 pM, and 2.8 × 105 low-affinity sites with a Kd of 30 nM. These binding sites are highly specific for thrombin. Half-maximal binding of 125I-labelled thrombin is achieved after 3 min at 37¸ C and 7 min at 4¸ C. The reversibly bound fraction of the ligand dissociates according to a biexponential time course with the rate constants 1—2 × 10−2 s−1 and 3—4 × 10−4 s−1. Part of the tracer remains cell-associated even after prolonged incubation, but all cell-associated radioactivity migrates as intact thrombin upon sodium dodecyl sulphate polyacrylamide gel electrophoresis. The bound thrombin is minimally endocytosed as judged by the resistance to pH 3-treatment. Cell-associated radioactivity dissociated from the cells binds just aswell in a receptor assay as tracer incubated in a conditioned medium under the same conditions, indicating the absence of a quantitatively important receptor-mediated degradation ofthe ligand.

scholarly journals Effects of carbon tetrachloride on isolated rat hepatocytes. Inhibition of protein and lipoprotein secretion

1979 ◽  
Vol 178 (2) ◽  
pp. 509-512 ◽  
Author(s):  
E Gravela ◽  
E Albano ◽  
M U Dianzani ◽  
G Poli ◽  
T F Slater
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Carbon Tetrachloride ◽  
Inhibitory Action ◽  
Rat Hepatocytes ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Isolated Rat Hepatocytes ◽  
Lipoprotein Secretion ◽  
Isolated Rat

The effects of carbon tetrachloride on protein and lipoprotein secretion, and on lipid peroxidation, have been investigated in isolated rat hepatocytes. It was found that although the free-radical scavenger promethazine completely suppressed the increased peroxidation produced by carbon tetrachloride, it had no effect on the inhibitory action of carbon tetrachloride on lipoprotein secretion. In consequence, the latter effect of carbon tetrachloride does not appear to be mediated through a peroxidative stage.

Characterization of Somatogenic and Lactogenic Binding Sites in Isolated Rat Hepatocytes

Endocrinology ◽  
1976 ◽  
Vol 99 (4) ◽  
pp. 1033-1045 ◽  
Author(s):  
MICHAEL B. RANKE ◽  
CHARLES A. STANLEY ◽  
ALFRED TENORE ◽  
DAVID RODBARD ◽  
ALFRED M. BONGIOVANNI ◽  
...  
Keyword(s):  
Binding Sites ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

scholarly journals Characterization of responses of isolated rat hepatocytes to ATP and ADP.

1985 ◽  
Vol 260 (29) ◽  
pp. 15789-15794 ◽  
Author(s):  
R Charest ◽  
P F Blackmore ◽  
J H Exton
Keyword(s):  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat
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