Synthesis and characterization of N-hydroxysuccinimide ester chemical affinity derivatives of asialoorosomucoid that covalently crosslink to galactosyl receptors on isolated rat hepatocytes

Biochemistry ◽  
1989 ◽  
Vol 28 (2) ◽  
pp. 600-610 ◽  
Author(s):  
Maryanne C. S. Herzig ◽  
Paul H. Weigel
Keyword(s):  
Rat Hepatocytes ◽  
Synthesis And Characterization ◽  
Chemical Affinity ◽  
Isolated Rat Hepatocytes ◽  
Derivatives Of ◽  
Isolated Rat

Characterization of the Binding of Bovine Thrombin to Isolated Rat Hepatocytes

1988 ◽  
Vol 60 (03) ◽  
pp. 419-427 ◽  
Author(s):  
Britta Weyer ◽  
Torben E Petersen ◽  
Ole Sonne
Keyword(s):  
Binding Sites ◽  
Time Course ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Prolonged Incubation ◽  
Receptor Assay ◽  
Isolated Rat

SummaryIsolated rat hepatocytes possess per cell 4,500 high-affinity binding sites for thrombin with a Kd of 30-40 pM, and 2.8 × 105 low-affinity sites with a Kd of 30 nM. These binding sites are highly specific for thrombin. Half-maximal binding of 125I-labelled thrombin is achieved after 3 min at 37¸ C and 7 min at 4¸ C. The reversibly bound fraction of the ligand dissociates according to a biexponential time course with the rate constants 1—2 × 10−2 s−1 and 3—4 × 10−4 s−1. Part of the tracer remains cell-associated even after prolonged incubation, but all cell-associated radioactivity migrates as intact thrombin upon sodium dodecyl sulphate polyacrylamide gel electrophoresis. The bound thrombin is minimally endocytosed as judged by the resistance to pH 3-treatment. Cell-associated radioactivity dissociated from the cells binds just aswell in a receptor assay as tracer incubated in a conditioned medium under the same conditions, indicating the absence of a quantitatively important receptor-mediated degradation ofthe ligand.

Characterization of Somatogenic and Lactogenic Binding Sites in Isolated Rat Hepatocytes

Endocrinology ◽  
1976 ◽  
Vol 99 (4) ◽  
pp. 1033-1045 ◽  
Author(s):  
MICHAEL B. RANKE ◽  
CHARLES A. STANLEY ◽  
ALFRED TENORE ◽  
DAVID RODBARD ◽  
ALFRED M. BONGIOVANNI ◽  
...  
Keyword(s):  
Binding Sites ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

scholarly journals Characterization of responses of isolated rat hepatocytes to ATP and ADP.

1985 ◽  
Vol 260 (29) ◽  
pp. 15789-15794 ◽  
Author(s):  
R Charest ◽  
P F Blackmore ◽  
J H Exton
Keyword(s):  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

scholarly journals Optimization of primary hepatocyte isolation for the pharmacological characterization of metabotropic glutamate receptor (mGluR) s ubtype 5: A study on Reduction

2021 ◽  
Vol 4 (s1) ◽  
Author(s):  
Clarissa Berardo ◽  
Andrea Ferrigno ◽  
Veronica Siciliano ◽  
Marta Cagna ◽  
Plinio Richelmi ◽  
...  
Keyword(s):  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Rat Hepatocytes ◽  
Pharmacological Characterization ◽  
Isolated Rat Hepatocytes ◽  
Metabotropic Glutamate ◽  
Hepatocyte Isolation ◽  
Collagenase Iv ◽  
Isolated Rat

To minimize the number of animals used during experiments, it is important to choose the suitable enzyme according to the final goal. In our work, we demonstrated the superiority of collagenase IV in the maintenance of functional transmembrane receptor, thus the pharmacological activity, in isolated rat hepatocytes.

Characterization of the slowly dissociable human growth hormone binding component of isolated rat hepatocytes

Biochemistry ◽  
1980 ◽  
Vol 19 (14) ◽  
pp. 3293-3300 ◽  
Author(s):  
David B. Donner ◽  
Jan Casadei ◽  
Louise Hartstein ◽  
Dwight Martin ◽  
Martin Sonenberg
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Rat Hepatocytes ◽  
Hormone Binding ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat ◽  
Binding Component
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