In this work there was evaluated the method of detection of methicillin
resistant Staphylococcus aureus (MRSA) by using two molecular and three
phenotypic tests in investigation procedure of 70 strains of S.aureus
isolated from animals. Recent findings of the new mecA homologue, mecALGA251,
minimise the significance of mecA gene presence detection as a confirmation
method of methicillin resistant Staphylococcus aureus identification. For
this reason, along with multiplex PCR set of primers(165rDNK, nuc, mecA) for
detection mecA gene, there was also used multiplex PCR set of primers (spa,
mecA, pvl, mecALGA251) for differentiation mecALGA251 from mecA, with
simultaneous detection of luk-PV and spa gene fragments. In all 70
investigated isolates there was detected the presence of specific 16 SrDNK
fragment and nuc gene which encodes a thermostable S. aureus nuclease, while
in 5 out of 70 S. aureus isolates, there was proven mecA gene presence using
two multiplex PCR tests. In the investigated strains there was determined
neither mecC (mecALGA251)gene presence, nor Panton Valentine Leukocidin
encoding gene. By application cefoxitin disk-diffusion, latex-agglutination
and two multiplex PCR tests, the identical results in identification 5
methicillin resistant out of 70 investigated S. aureus strains were obtained.
In our investigation there was determined a complete correlation between the
results of phenotypic and genotypic identification of methicillin resistant
S. aureus.
VALIDATION OF MULTIPLEX PCR STRATEGY FOR SIMULTANEOUS DETECTION AND IDENTIFICATION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
