First complex cell had extra DNA

Bioorthogonal chemistry is a mainstay of chemoproteomic sample preparation workflows. While numerous transformations are now available, chemoproteomic studies still rely overwhelmingly on copper-catalyzed azide –alkyne cycloaddition (CuAAC) or 'click' chemistry. Here we demonstrate that gel-based activity-based protein profiling (ABPP) and mass-spectrometry-based chemoproteomic profiling can be conducted using Suzuki–Miyaura cross-coupling. We identify reaction conditions that proceed in complex cell lysates and find that Suzuki –Miyaura cross-coupling and CuAAC yield comparable chemoproteomic coverage. Importantly, Suzuki–Miyaura is also compatible with chemoproteomic target deconvolution, as demonstrated using structurally matched probes tailored to react with the cysteine protease caspase-8. Uniquely enabled by the observed orthogonality of palladium-catalyzed cross-coupling and CuAAC, we combine both reactions to achieve dual protein labeling.

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Realistic Modeling of Simple and Complex Cell Tuning in the HMAX Model, and Implications for Invariant Object Recognition in Cortex

10.21236/ada459692 ◽

2004 ◽

Cited By ~ 56

Author(s):

Thomas Serre ◽

Maximilian Riesenhuber

Keyword(s):

Object Recognition ◽

Complex Cell ◽

Realistic Modeling ◽

Invariant Object Recognition ◽

Simple And Complex Cell

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Cellular Senescence and Mammalian Healthspan

Innovation in Aging ◽

10.1093/geroni/igaa057.2655 ◽

2020 ◽

Vol 4 (Supplement_1) ◽

pp. 742-742

Author(s):

Judith Campisi

Keyword(s):

Growth Factors ◽

Cell Fate ◽

Cellular Senescence ◽

Complex Cell ◽

Transgenic Mouse Models ◽

Bioactive Lipids ◽

Age Related ◽

Mammalian Tissues ◽

Secretory Phenotype ◽

Near Future

Abstract Cellular senescence is a complex cell fate, often induced by stress or damage, that can be beneficial or deleterious, depending on the physiological context and age of the organism. A prominent feature of senescent cells is a multi-faceted senescence-associated secretory phenotype (SASP), which includes growth factors, cytokine and chemokines, growth factors, proteases, bioactive lipids and metabolites. Senescent cells increase with age in most, if not all, mammalian tissues. Through the use of transgenic mouse models, senescent cells are now known to causally drive numerous age-related pathologies, largely through the SASP. Eliminating senescent cells, genetically or through the use of senolytic/senomorphic agents, can improve the health span, at least in mice, and hold promise for extension to humans in the near future.

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Systems Biology and grid technologies: Challenges for understanding complex cell signaling networks

Future Generation Computer Systems ◽

10.1016/j.future.2006.10.001 ◽

2007 ◽

Vol 23 (3) ◽

pp. 428-434 ◽

Cited By ~ 4

Author(s):

Irina Strizh ◽

Alexei Joutchkov ◽

Nikolay Tverdokhlebov ◽

Sergey Golitsyn

Keyword(s):

Systems Biology ◽

Cell Signaling ◽

Signaling Networks ◽

Complex Cell ◽

Cell Signaling Networks

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Ancient, highly conserved proteins from a LUCA with complex cell biology provide evidence in support of the nuclear compartment commonality (NuCom) hypothesis

Research in Microbiology ◽

10.1016/j.resmic.2017.01.001 ◽

2017 ◽

Vol 168 (5) ◽

pp. 395-412 ◽

Cited By ~ 6

Author(s):

James T. Staley ◽

John A. Fuerst

Keyword(s):

Cell Biology ◽

Complex Cell ◽

Nuclear Compartment

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Encoding of Binocular Disparity by Complex Cells in the Cat's Visual Cortex

Journal of Neurophysiology ◽

10.1152/jn.1997.77.6.2879 ◽

1997 ◽

Vol 77 (6) ◽

pp. 2879-2909 ◽

Cited By ~ 146

Author(s):

Izumi Ohzawa ◽

Gregory C. Deangelis ◽

Ralph D. Freeman

Keyword(s):

Visual Cortex ◽

Striate Cortex ◽

Binocular Disparity ◽

Substantial Reduction ◽

Energy Model ◽

Simple Type ◽

Correspondence Problem ◽

Complex Cell ◽

Stimulus Contrast ◽

Complex Cells

Ohzawa, Izumi, Gregory C. DeAngelis, and Ralph D. Freeman. Encoding of binocular disparity by complex cells in the cat's visual cortex. J. Neurophysiol. 77: 2879–2909, 1997. To examine the roles that complex cells play in stereopsis, we have recorded extracellularly from isolated single neurons in the striate cortex of anesthetized paralyzed cats. We measured binocular responses of complex cells using a comprehensive stimulus set that encompasses all possible combinations of positions over the receptive fields for the two eyes. For a given position combination, stimulus contrast could be the same for the two eyes (2 bright or 2 dark bars) or opposite (1 bright and 1 dark). These measurements provide a binocular receptive field (RF) profile that completely characterizes complex cell responses in a joint domain of left and right stimulus positions. Complex cells typically exhibit a strong selectivity for binocular disparity, but are only broadly selective for stimulus position. For most cells, selectivity for disparity is more than twice as narrow as that for position. These characteristics are highly desirable if we assume that a disparity sensor should exhibit position invariance while encoding small changes in stimulus depth. Complex cells have nearly identical binocular RFs for bright and dark stimuli as long as the sign of stimulus contrast is the same for the two eyes. When stimulus contrast is opposite, the binocular RF also is inverted such that excitatory subregions become suppressive. We have developed a disparity energy model that accounts for the behavior of disparity-sensitive complex cells. This is a hierarchical model that incorporates specific constraints on the selection of simple cells from which a complex cell receives input. Experimental data are used to examine quantitatively predictions of the model. Responses of complex cells generally agree well with predictions of the disparity energy model. However, various types of deviations from the predictions also are found, including a highly elongated excitatory region beyond that supported by a single energy mechanism. Complex cells in the visual cortex appear to provide a next level of abstraction in encoding information for stereopsis based on the activity of a group of simple-type subunits. In addition to exhibiting narrow disparity tuning and position invariance, these cells seem to provide a partial solution to the stereo correspondence problem that arises in complex natural scenes. Based on their binocular response properties, these cells provide a substantial reduction in the complexity of the correspondence problem.

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