Characterization of isolated in Taiwan by immunofluorescence and restriction fragment length polymorphism analyses

1997 ◽  
Vol 150 (2) ◽  
pp. 225-231 ◽  
Author(s):  
A TAMURA ◽  
N OHASHI ◽  
Y KOYAMA ◽  
M FUKUHARA ◽  
F KAWAMORI ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Fragment Length

Characterization of DR blank alleles by restriction fragment length polymorphism (RFLP)

2008 ◽  
Vol 29 (3) ◽  
pp. 154-159 ◽  
Author(s):  
E. Keller ◽  
A. Andreas-Zietz ◽  
A. McNicholas ◽  
A. Grooms ◽  
S. Scholz ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Fragment Length

Cloning, Sequencing, and Characterization of Genomic Subtracted Sequences from Listeria monocytogenes

1999 ◽  
Vol 65 (12) ◽  
pp. 5427-5430
Author(s):  
Fone-Mao Wu ◽  
Peter M. Muriana
Keyword(s):  
Listeria Monocytogenes ◽  
Sequence Analysis ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Individual Sequences ◽  
Restriction Fragment Length

ABSTRACT Individual sequences of a genomic subtracted, PCR-amplified, mixed-sequence probe (GS probe) were cloned and sequenced. The GS probe differentiated restriction fragment length polymorphism patterns forListeria monocytogenes but did not hybridize with members of other bacterial genera. Sequence analysis identified severalL. monocytogenes sequences already present in the GenBank database; the putative identities of other sequences were inferred from homology data, and still other sequences did not exhibit significant levels of homology with any GenBank sequences.

scholarly journals Characterization of the Archaeal Community in a Minerotrophic Fen and Terminal Restriction Fragment Length Polymorphism-Directed Isolation of a Novel Hydrogenotrophic Methanogen

2008 ◽  
Vol 74 (7) ◽  
pp. 2059-2068 ◽  
Author(s):  
Hinsby Cadillo-Quiroz ◽  
Erica Yashiro ◽  
Joseph B. Yavitt ◽  
Stephen H. Zinder
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
New York State ◽  
Rflp Analysis ◽  
Archaeal Community ◽  
Restriction Fragment Length

ABSTRACT Minerotrophic fen peatlands are widely distributed in northern latitudes and, because of their rapid turnover of organic matter, are potentially larger sources of atmospheric methane than bog peatlands per unit area. However, studies of the archaeal community composition in fens are scarce particularly in minerotrophic sites. Several 16S rRNA-based primer sets were used to obtain a broad characterization of the archaeal community in a minerotrophic fen in central New York State. A wide archaeal diversity was observed in the site: 11 euryarchaeal and 2 crenarchaeal groups, most of which were uncultured. The E1 group, a novel cluster in the order Methanomicrobiales, and Methanosaetaceae were the codominant groups in all libraries and results of terminal restriction fragment length polymorphism (T-RFLP) analysis. Given its abundance and potential hydrogenotrophic methane contribution, the E1 group was targeted for culture attempts with a low-ionic-strength medium (PM1). Initial attempts yielded Methanospirillum-dominated cultures. However, by incorporating a T-RFLP analysis as a quick selection tool for treatments and replicates, we were able to select an enrichment dominated by E1. Further dilutions to 10−9 and tracking with T-RFLP yielded a strain named E1-9c. E1-9c is a novel coccoid hydrogenotrophic, mesophilic, slightly acidophilic methanogen and is highly sensitive to Na2S concentrations (requires <0.12 mM for growth). We propose E1-9c as the first representative of a novel genus in the Methanomicrobiales order.

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