A comparative study of serological tests for use in the bovine Herpesvirus 1 eradication programme in The Netherlands

Transmission of bovine herpesvirus 1 (BHV1) within and between herds was studied on the island of Ameland, The Netherlands. There were 50 herds with 3300 head of cattle on the island. Herds were divided into three groups: (1) only containing seronegative cattle, (2) containing seronegative cattle and vaccinated seropositive cattle, and (3) containing only vaccinated cattle. All 23 herds in groups 1 and 2 were monitored. Three major outbreaks of BHV1 infections were observed due to the introduction of infectious cattle. Another major outbreak was most likely induced by reactivation of latent BHV1 in seropositive cattle. The basic reproduction ratio within these herds was estimated at least 4. Only one of these outbreaks led to three secondary outbreaks in susceptible herds in which all cattle were seronegative. These outbreaks were most likely due to respectively, direct animal contact, human transmission, and aerogenic transmission. The basic reproduction ratio between herds in this study was estimated to be 0·6.

Download Full-text

COMPARATIVE STUDY OF THE CLASTOGENIC ACTION OF BOVINE HERPESVIRUS 1 (BOHV-1) IN PRIMARY AND ESTABLISHED CELL CULTURES

VIRUS Reviews & Research ◽

10.17525/vrr.v15i1.38 ◽

2010 ◽

Vol 15 (1) ◽

Author(s):

Edson G. Rocha ◽

Luciano Belloti ◽

Danielle L. Pavão ◽

Karen G. Damasco ◽

Ricardo Harakava ◽

...

Keyword(s):

Comparative Study ◽

Cell Cultures ◽

Bovine Herpesvirus ◽

Bovine Herpesvirus 1 ◽

Established Cell ◽

Herpesvirus 1

Download Full-text

Comparative study on sixteen enzyme-linked immunosorbent assays for the detection of antibodies to bovine herpesvirus 1 in cattle

Veterinary Microbiology ◽

10.1016/0378-1135(93)90112-k ◽

1993 ◽

Vol 35 (1-2) ◽

pp. 11-21 ◽

Cited By ~ 7

Author(s):

J.A. Kramps ◽

S. Quak ◽

K. Weerdmeester ◽

J.T. van Oirschot

Keyword(s):

Comparative Study ◽

Bovine Herpesvirus ◽

Bovine Herpesvirus 1 ◽

Herpesvirus 1 ◽

Immunosorbent Assays

Download Full-text

An evaluation of five serological tests for the detection of antibody to bovine herpesvirus 1 in vaccinated and experimentally infected cattle

Research in Veterinary Science ◽

10.1016/s0034-5288(18)30634-9 ◽

1986 ◽

Vol 41 (3) ◽

pp. 378-382 ◽

Cited By ~ 10

Author(s):

S. EDWARDS ◽

S.B. WOODS ◽

D.G. WESTCOTT ◽

M. EMMERSON ◽

P.C. JONES ◽

...

Keyword(s):

Bovine Herpesvirus ◽

Infected Cattle ◽

Serological Tests ◽

Bovine Herpesvirus 1 ◽

Herpesvirus 1

Download Full-text

Prokaryotic expression of a truncated form of bovine herpesvirus 1 glycoprotein E (gE) and its use in an ELISA for gE antibodies

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2013000100008 ◽

2013 ◽

Vol 33 (1) ◽

pp. 41-46 ◽

Cited By ~ 2

Author(s):

Stephan A.M. Oliveira ◽

Mário Celso S. Brum ◽

Deniz Anziliero ◽

Odir Dellagostin ◽

Rudi Weiblen ◽

...

Keyword(s):

Bovine Herpesvirus ◽

Truncated Form ◽

Serological Tests ◽

Bovine Herpesvirus 1 ◽

Glycoprotein E ◽

High Identity ◽

E Coli ◽

Amino Terminal ◽

Herpesvirus 1 ◽

Escherichia Coli Expression

This article describes the expression of a truncated form of bovine herpesvirus 1 (BoHV-1) glycoprotein E (gE) for use as immunodiagnostic reagent. A 651 nucleotide fragment corresponding to the amino-terminal third (217 amino acids) of BoHV-1 gE - that shares a high identity with the homologous BoHV-5 counterpart - was cloned as a 6×His-tag fusion protein in an Escherichia coli expression vector. A soluble protein of approximately 25 kDa purified from lysates of transformed E. coli was recognized in Western blot (WB) by anti-6xHis-tag and anti-BoHV-1 gE monoclonal antibodies. In addition, the recombinant protein was specifically recognized in WB by antibodies present in the sera of cattle seropositive to BoHV-1 and BoHV-5. An indirect ELISA using the expressed protein as coating antigen performed comparably to a commercial anti-gE ELISA and was able to differentiate serologically calves vaccinated with a gE-deleted BoHV-5 strain from calves infected with BoHV-1. Thus, the truncated gE may be useful for serological tests designed to differentiate BoHV-1/BoHV-5 infected animals from those vaccinated with gE-negative marker vaccines.

Download Full-text