Lipid peroxidation as a consequence of aluminum toxicity in human skin fibroblast cultures: Prevention by superoxide dismutase + catalase, Vitamin E and Vitamin C

1998 ◽  
Vol 95 ◽  
pp. 54
Author(s):  
Rachid Anane ◽  
Edmond Ekué Creppy
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Vitamin E ◽  
Vitamin C ◽  
Human Skin ◽  
Skin Fibroblast ◽  
Aluminum Toxicity ◽  
Human Skin Fibroblast ◽  
Fibroblast Cultures

Lipid peroxidation as pathway of aluminium cytotoxicity in human skin fibroblast cultures: Prevention by superoxide dismutase+catalase and vitamins E and C

2001 ◽  
Vol 20 (9) ◽  
pp. 477-481 ◽  
Author(s):  
R Anane ◽  
E E Creppy
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Human Skin ◽  
Thiobarbituric Acid ◽  
Aluminium Toxicity ◽  
Free Radical Scavengers ◽  
Human Skin Fibroblast ◽  
Fibroblast Cultures ◽  
Vitamins E And C ◽  
Ldh Release

Lipid peroxidation is one of the main manifestations of oxidative damage and has been found to play an important role in the toxicity and carcinogenicity of many xenobiotics. In the present study, we investigated the possible induction of lipid peroxidation by aluminium in human foreskin fibroblast cultures by assaying the malondialde hyde (MDA) produced inside the cells. The MDA–thiobarbituric acid (TBA) adduct was assayed by HPLC using fluorometric quantification after extraction in n-butanol. Lactate dehydrogenase (LDH) release was used as a marker of aluminium toxicity. MDA production was significantly increased after 24 h incubation with aluminium and paralleled LDH release. Superoxide dismutase (SOD)+catalase and vitamins C and E added in the culture medium as oxygen radical and free radical scavengers were efficient in preventing MDA production by aluminium, indicating that oxidative processes are one of the main pathways whereby this metal induces cytotoxicity. The latter is also largely prevented, thus confirming the link between oxidative stress induced by aluminium and its cytotoxicity in human skin fibroblasts. Human & Experimental Toxicology (2001) 20, 477–481.

Modifications of proteoglycans produced by human skin fibroblast cultures during replicative senescence

1993 ◽  
Vol 11 (4) ◽  
pp. 263-269 ◽  
Author(s):  
A. Passi ◽  
R. Albertini ◽  
A. Bardoni ◽  
S. Rindi ◽  
R. Salvini ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Fibroblast ◽  
Replicative Senescence ◽  
Human Skin Fibroblast ◽  
Fibroblast Cultures

Effects of chromosome constitution on growth and longevity of human skin fibroblast cultures

1980 ◽  
Vol 7 (2) ◽  
pp. 141-154 ◽  
Author(s):  
H. Hoehn ◽  
M. Simpson ◽  
E. M. Bryant ◽  
P. S. Rabinovitch ◽  
D. Salk ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Fibroblast ◽  
Chromosome Constitution ◽  
Human Skin Fibroblast ◽  
Fibroblast Cultures

The concentration-dependent effect of anethole on collagen, MMP-2 and GAG in human skin fibroblast cultures

2019 ◽  
Vol 64 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Edyta Andrulewicz-Botulińska ◽  
Iwona Kuźmicz ◽  
Jolanta Nazaruk ◽  
Joanna Wosek ◽  
Anna Galicka
Keyword(s):  
Human Skin ◽  
Skin Fibroblast ◽  
Human Skin Fibroblast ◽  
Dependent Effect ◽  
Fibroblast Cultures
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