Cytochrome P450 (CYP) isoenzymes in cultured precision-cut human liver slices: Effects of oxygen tension

1998 ◽  
Vol 95 ◽  
pp. 99
Author(s):  
C. Lerche-Langrand ◽  
V. Moronvalle-Halley ◽  
D. Chevalier ◽  
D. Hoët ◽  
D. Leroy ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Oxygen Tension ◽  
Human Liver ◽  
Liver Slices ◽  
Human Liver Slices

Induction of cytochrome P450 isoenzymes in cultured precision-cut rat and human liver slices

Xenobiotica ◽  
1996 ◽  
Vol 26 (3) ◽  
pp. 297-306 ◽  
Author(s):  
B. G. Lake ◽  
C. Charzat ◽  
J. M. Tredger ◽  
A. B. Renwick ◽  
J. A. Beamand ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Human Liver ◽  
Liver Slices ◽  
Human Liver Slices

Evaluation of Human Liver Slices and Reporter Gene Assays as Systems for Predicting the Cytochrome P450 Induction Potential of Drugs in Vivo in Humans

2006 ◽  
Vol 23 (1) ◽  
pp. 56-69 ◽  
Author(s):  
Kajsa P. Persson ◽  
Susanne Ekehed ◽  
Charlotta Otter ◽  
E. S. Mareike Lutz ◽  
Jane McPheat ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Reporter Gene ◽  
Human Liver ◽  
Liver Slices ◽  
Cytochrome P450 Induction ◽  
Human Liver Slices ◽  
Reporter Gene Assays

Human Liver Slices Express the Same Lidocaine Biotransformation Rate as Isolated Human Hepatocytes

1993 ◽  
Vol 21 (4) ◽  
pp. 466-469
Author(s):  
Peter Olinga ◽  
Marjolijn T. Merema ◽  
Dick K.F. Meijer ◽  
Maarten J.H. Slooff ◽  
Geny M.M. Groothuis
Keyword(s):  
Cytochrome P450 ◽  
Individual Differences ◽  
Human Liver ◽  
Isolated Hepatocytes ◽  
Human Hepatocytes ◽  
Isolated Cells ◽  
Liver Slices ◽  
Human Livers ◽  
Optimal Incubation ◽  
Human Liver Slices

In order to investigate whether liver slices are a valuable tool for the assessment of drug metabolism in human liver, we compared the phase I metabolism of lidocaine in human liver slices and hepatocytes prepared from three human livers. Lidocaine is mainly metabolised to monoethylglycinexykdide (MEGX) via a cytochrome P450-mediated N-deethylation. The results indicate that the three livers showed considerable inter-individual differences in the rate of formation of MEGX, and that this difference was equally reflected in slices and isolated cells. The use of liver slices is still under development, and optimal incubation conditions still need to be assessed. However, these results suggest that, in slices of 200–300μm thickness, virtually all hepatocytes are involved in the biotransformation of lidocaine, and that the metabolic activity is preserved equally well as in isolated hepatocytes.

Induction of Cytochrome P450 Enzymes in Cultured Precision-Cut Human Liver Slices

2003 ◽  
Vol 31 (3) ◽  
pp. 282-288 ◽  
Author(s):  
Robert J. Edwards ◽  
Roger J. Price ◽  
Patricia S. Watts ◽  
Anthony B. Renwick ◽  
J. Michael Tredger ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Human Liver ◽  
Cytochrome P450 Enzymes ◽  
Liver Slices ◽  
Human Liver Slices

Cryopreservation of pig and human liver slices

Cryobiology ◽  
1991 ◽  
Vol 28 (2) ◽  
pp. 131-142 ◽  
Author(s):  
Robyn Fisher ◽  
Charles W. Putnam ◽  
Lawrence J. Koep ◽  
I.Glenn Sipes ◽  
A.Jay Gandolfi ◽  
...  
Keyword(s):  
Human Liver ◽  
Liver Slices ◽  
Human Liver Slices

THE METABOLISM OF ALDOSTERONE BY SURVIVING DOG AND HUMAN LIVER SLICES

1960 ◽  
Vol 38 (1) ◽  
pp. 739-756
Author(s):  
Thomas Sandor ◽  
Wojciech J. Nowaczynski ◽  
Jacques Genest
Keyword(s):  
Human Liver ◽  
Chemical Characterization ◽  
Liver Slices ◽  
Metabolic Products ◽  
Reducing Substances ◽  
Dog Liver ◽  
Human Liver Slices ◽  
Acetate Form

Surviving dog liver slices were incubated with d,l-aldosterone-21-monoacetate, d,l-aldosterone, d-aldosterone, and d-aldosterone-21-C14. Human liver slices were incubated with d,l-aldosterone-21-monoacetate and d-aldosterone. The incubations were performed in a Krebs–Ringer–phosphate medium (pH 7.4), with 200 mg glucose added per 100 ml of medium, at a temperature of 37 °C. After incubation, the medium was extracted with chloroform and the crude extract extensively fractionated on column and paper chromatographic systems. In addition to free aldosterone, four metabolic products were isolated, two ring A reduced α-ketolic and two ultraviolet absorbing, non-reducing substances. The partial chemical characterization of these metabolites was attempted. The search for aldosterone metabolites in human urine resulted in the isolation of a substance in acetate form from the urine of a patient suffering from primary aldosteronism which may be identical with one of the ring A reduced metabolites obtained in the in vitro experiments.

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