Inhibition of cytochrome P450 2E1 expression by oleanolic acid: hepatoprotective effects against carbon tetrachloride-induced hepatic injury

1999 ◽  
Vol 105 (3) ◽  
pp. 215-222 ◽  
Author(s):  
Hye Gwang Jeong
Keyword(s):  
Cytochrome P450 ◽  
Carbon Tetrachloride ◽  
Oleanolic Acid ◽  
Hepatic Injury ◽  
Cytochrome P450 2E1 ◽  
Hepatoprotective Effects

scholarly journals Increased Cytotoxicity of Carbon Tetrachloride in a Human Hepatoma Cell Line Overexpressing Cytochrome P450 2E1

2002 ◽  
Vol 30 (4) ◽  
pp. 400-405 ◽  
Author(s):  
S Takahashi ◽  
T Takahashi ◽  
S Mizobuchi ◽  
M Matsumi ◽  
K Morita ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cytochrome P450 ◽  
Carbon Tetrachloride ◽  
Cell Line ◽  
Hepatoma Cell ◽  
Mrna Level ◽  
Hepatoma Cell Line ◽  
Cytochrome P450 2E1 ◽  
Hsp70 Mrna ◽  
Potential Marker

Cytotoxic free radicals generated during the metabolism of carbon tetrachloride by cytochrome P450 2E1 (CYP2E1) are thought to cause hepatotoxicity. Here, the cytotoxic effects of carbon tetrachloride in a liver cell line expressing CYP2E1 (HLE/2E1) are compared with those in the mother cell line (HLE). The effects of carbon tetrachloride on the gene expression of HSP70, a potential marker of oxidative stress, were also examined. The viability of HLE/2E1 cells after exposure to carbon tetrachloride was significantly decreased compared with that of HLE cells. Northern blot analysis revealed that the HSP70 mRNA level was significantly increased after carbon tetrachloride treatment in both cell lines, while the magnitude of its increase was much greater in HLE/2E1 cells than in HLE cells. These results suggest that the oxidative stress induced by CYP2E1 plays an important role in the increase in cytotoxicity of carbon tetrachloride in CYP2E1-overexpressing cells.

scholarly journals Dietary tomato powder inhibits alcohol-induced hepatic injury by suppressing cytochrome p450 2E1 induction in rodent models

2015 ◽  
Vol 572 ◽  
pp. 81-88 ◽  
Author(s):  
Camilla P. Stice ◽  
Chun Liu ◽  
Koichi Aizawa ◽  
Andrew S. Greenberg ◽  
Lynne M. Ausman ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Hepatic Injury ◽  
Rodent Models ◽  
Cytochrome P450 2E1 ◽  
Tomato Powder
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