Development of a gas chromatographic—mass spectrometric method using multiple analytes for the confirmatory analysis of anabolic steroid residues in horse urine

1986 ◽  
Vol 383 ◽  
pp. 1-8 ◽  
Author(s):  
E. Houghton ◽  
M.C. Dumasia ◽  
P. Teale ◽  
M.S. Moss ◽  
Sharon Sinkins
Keyword(s):  
Anabolic Steroid ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Multiple Analytes ◽  
Confirmatory Analysis ◽  
Chromatographic Mass

Melatonin secretion in humans assessed by measuring its metabolite, 6-sulfatoxymelatonin.

1987 ◽  
Vol 33 (8) ◽  
pp. 1343-1348 ◽  
Author(s):  
C J Bojkowski ◽  
J Arendt ◽  
M C Shih ◽  
S P Markey
Keyword(s):  
Urinary Excretion ◽  
Room Temperature ◽  
Area Under The Curve ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Plasma Samples ◽  
Melatonin Secretion ◽  
Short Pulses ◽  
Chromatographic Mass

Abstract Comparing a direct radioimmunoassay for 6-sulfatoxymelatonin (aMT6s) with an established gas chromatographic/mass spectrometric method for 6-hydroxymelatonin, we found a good correlation r = 0.94 (P less than 0.001, n = 100). aMT6s was stable, both in urine and plasma samples, without preservative, for at least two years at -20 degrees C and for five days at room temperature. Urinary excretion of aMT6s showed considerable inter-individual differences; however, the aMT6s excretion of any one individual was consistent over a four-day period, as assessed by continuous collection from 18 normal volunteers. Total 24-h urinary excretion of aMT6s was significantly correlated with the area under the curve of the respective profiles for plasma melatonin (r = 0.75, P = 0.0002) and plasma aMT6s (r = 0.70, P = 0.0005) for 22 healthy volunteers. At 24:00 h and 03:00 h, sampling plasma at 30-s intervals provided no evidence for episodic secretion (in short pulses) of either melatonin or aMT6s.

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