Acetobacterium wieringae sp. nov., a new species producing acetic acid from molecular hydrogen and carbon dioxide

The photocatalytic decomposition of aqueous acetic acid into molecular hydrogen, carbon dioxide, and hydrocarbons employing platinized titania (Pt/TiO2) as a photocatalyst has been studied.

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A New Chemolithoautotrophic Arsenite-Oxidizing Bacterium Isolated from a Gold Mine: Phylogenetic, Physiological, and Preliminary Biochemical Studies

Applied and Environmental Microbiology ◽

10.1128/aem.66.1.92-97.2000 ◽

2000 ◽

Vol 66 (1) ◽

pp. 92-97 ◽

Cited By ~ 312

Author(s):

Joanne M. Santini ◽

Lindsay I. Sly ◽

Roger D. Schnagl ◽

Joan M. Macy

Keyword(s):

Carbon Dioxide ◽

New Species ◽

Minimal Medium ◽

Doubling Time ◽

Northern Territory ◽

Gold Mine ◽

Gram Negative ◽

Biochemical Studies ◽

Optimum Ph ◽

A New Species

ABSTRACT A previously unknown chemolithoautotrophic arsenite-oxidizing bacterium has been isolated from a gold mine in the Northern Territory of Australia. The organism, designated NT-26, was found to be a gram-negative motile rod with two subterminal flagella. In a minimal medium containing only arsenite as the electron donor (5 mM), oxygen as the electron acceptor, and carbon dioxide-bicarbonate as the carbon source, the doubling time for chemolithoautotrophic growth was 7.6 h. Arsenite oxidation was found to be catalyzed by a periplasmic arsenite oxidase (optimum pH, 5.5). Based upon 16S rDNA phylogenetic sequence analysis, NT-26 belongs to theAgrobacterium/Rhizobium branch of the α-Proteobacteria and may represent a new species. This recently discovered organism is the most rapidly growing chemolithoautotrophic arsenite oxidizer known.

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The ultrastructure of the major species of an enriched methanogenic culture utilizing acetic acid

Canadian Journal of Microbiology ◽

10.1139/m79-122 ◽

1979 ◽

Vol 25 (7) ◽

pp. 826-832 ◽

Cited By ~ 7

Author(s):

J. Ross Colvin ◽

L. C. Sowden ◽

L. van den Berg

Keyword(s):

Acetic Acid ◽

New Species ◽

Variable Thickness ◽

Cell Walls ◽

Cytoplasmic Membrane ◽

Lead Ions ◽

Outer Leaflet ◽

Major Species ◽

Thin Portion ◽

A New Species

The ultrastructure of the cells of the major component of an enriched culture of a presumed methanogen which utilized acetic acid was studied by transmission and scanning election microscopy. The filaments were composed of Gram-positive, rod-shaped cells, 1–2 μm in length and about 0.5 μm in breadth, attached end to end. Septa between cells were complex, with a central, electron-dense sheet which had a spherical enlargement in the center separating the cell walls. The cells walls themselves were of variable thickness with a light, fluffy, thin portion on the outside and a denser, thicker portion within. They contain a series of rings stacked side by side which are composed of material that stains strongly and positively with phosphotungstate ion. The cytoplasmic membrane of these cells had an outer leaflet which stains more densely with uranium and lead ions than the inner leaflet. There were no recognizable organelles in the cytoplasm other than ribosomes. It is shown in these observations that the presumed methanogen may likely be a new species.

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