Determination of LY295501 in human plasma by reverse phase HPLC with UV detection

1999 ◽  
Vol 20 (5) ◽  
pp. 799-805
Author(s):  
M Berna ◽  
P Wood
Keyword(s):  
Human Plasma ◽  
Uv Detection ◽  
Reverse Phase Hplc ◽  
Reverse Phase

scholarly journals Determination of Valdecoxib in Human Plasma Using Reverse Phase HPLC

2011 ◽  
Vol 8 (2) ◽  
pp. 875-881
Author(s):  
Prafulla Kumar Sahu ◽  
K. Ravi Sankar ◽  
M. Mathrusri Annapurna
Keyword(s):  
Human Plasma ◽  
Perchloric Acid ◽  
Mobile Phase ◽  
Internal Standard ◽  
Clinical Settings ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Extraction Recovery ◽  
Rp Hplc

An RP-HPLC analytical method for estimation of valdecoxib in human plasma was developed and validated. Protein precipitation and valdecoxib extraction from plasma (200 μL) was carried out by adding 800 μL perchloric acid (5%, v/v in water) containing nimesulide as the internal standard followed by vortex mixing and centrifugation. The supernatant (20 μL) was then injected onto an ODS C18(25 cm×4.6 mm) column from Shimadzu. The mobile phase comprised of acetonitrile and water (35: 65) with a total run time of 12 min and the wavelength of the detector was set at 244 nm. The extraction recovery of valdecoxib from plasma was >95% and the calibration curve was linear (r2= 0.999) over valdecoxib concentrations ranging from 20 to 1400 μg/mL (n= 10). The method had an accuracy of >92% and LOD and LLOQ of 3.58 μg/mL and 13.45 μg/mL respectively. The method reported is simple, reliable, precise, accurate and has the capability of being used for determination of Valdecoxib in clinical settings.

scholarly journals HPLC methods for the determinatiuon of acetyl- and benzoyl-tiazofurin in rat plasma

2004 ◽  
Vol 69 (1) ◽  
pp. 69-75
Author(s):  
Ljiljana Petrovic ◽  
Vesna Piperski ◽  
Slavica Ristic ◽  
Jelena Tasic ◽  
Vesna Matovic ◽  
...  
Keyword(s):  
Particle Size ◽  
Rat Plasma ◽  
Uv Detection ◽  
Isocratic Elution ◽  
Hydrogen Phosphate ◽  
Linear Calibration ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Detection And Quantification

Reverse-phase HPLC methods for determination of 5?-O-acetyl-tiazofurin (AT) and 5?-O-benzoyl-tiazofurin (BT) in rat plasma were developed and validated in terms of specificity, linearity, precision, accuracy and sensitivity. Linear calibration curves were constructed in the concentration range of 2.50 ? 100.00 ?mol/L for both compounds. The separations were achieved on a Supelcosyl LC-18-DB analytical column (250x4.6 mm; 5_?m particle size) by isocratic elution, with a mixture of 0.1Mdisodium hydrogen phosphate ? methanol. UV detection was performed at a wavelength of 254 nm. The proposed methods enable the detection and quantification of nanomolar concentrations of AT and BT in rat plasma.

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