Isolation, purification and partial characterization of antibacterial activities produced by a newly isolated Streptomyces sp. US24 strain

2003 ◽  
Vol 154 (5) ◽  
pp. 345-352 ◽  
Author(s):  
Lotfi Mellouli ◽  
Raoudha Ben Ameur-Mehdi ◽  
Samiha Sioud ◽  
Mansour Salem ◽  
Samir Bejar
Keyword(s):  
Antibacterial Activities ◽  
Partial Characterization ◽  
Streptomyces Sp

scholarly journals ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM SOIL OF AD-DAWADMI, SAUDI ARABIA AND SCREENING THEIR ANTIBACTERIAL ACTIVITIES

2017 ◽  
Vol 9 (10) ◽  
pp. 276 ◽  
Author(s):  
Gouse Basha Sheik ◽  
Muazzam Sheriff Maqbul ◽  
Gokul Shankar S. ◽  
Ranjith M S
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Activities ◽  
Soil Samples ◽  
Streptomyces Sp ◽  
Crude Extracts ◽  
Test Organisms ◽  
Inhibition Zones

Objective: To isolate and characterize novel actinomycetes and to evaluate their antibacterial activity against drug-resistant pathogenic bacteriaMethods: In the present study, 19 soil samples were collected from different localities of Ad-Dawadmi, Saudi Arabia. Actinomycetes were isolated from these samples using serial dilution and plating method on Actinomycetes isolation agar supplemented with nalidixic acid and actidione to inhibit bacteria and fungi. Crude extracts of potential actinomycetes were produced by submerged fermentation. The antimicrobial activity of crude extracts of actinomycetes was tested against different bacteria using the agar well diffusion method. Characterization of the isolates was done by morphological, physiological and biochemical methods.Results: A total of 9 (47%) isolates of actinomycetes were isolated from 19 different soil samples tested. Among them, 4 (44%) isolates confirmed as Streptomyces sp. showed potential antimicrobial activity against one or more test organisms. Crude extracts were made from these 4 actinomycetes isolates(DOM1, DOM3, DP3, DP4)and tested for their antibacterial activities against 4 different clinical bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus). Crude extract from DP3 isolate showed highest antibacterial activity against all the four test organisms (28 mm, 21 mm, 20 mm and 18 mm) respectively and DP4 showed lowest antibacterial activity against all the four test organisms (14 mm, 12 mm, 0 mm, 6 mm) respectively. The highest zone of inhibition was shown by DP3 against Staphylococcus aureus (28 mm) and Escherichia coli was resistant for DP4. Most of the Inhibition zones produced by crude extracts showed significant differences when compared with control, tested against test organisms (P<0.05). Inhibition zones produced by DP3 and DOM1 against Staphylococcus aureus were 28 mm and 23 mm, respectively which were strong active when compared with control Ciprofloxacin (18 mm).Conclusion: Further studies for purification of bioactive metabolites and molecular characterization analysis of isolated Streptomyces sp. are in progress which would be helpful in discovering novel compounds of commercial value.

Production and partial characterization of thermophilic proteases from Streptomyces sp. isolated from Brazilian cerrado soil

2004 ◽  
Vol 34 (3-4) ◽  
pp. 354-358 ◽  
Author(s):  
L.A.I De Azeredo ◽  
D.M.G Freire ◽  
R.M.A Soares ◽  
S.G.F Leite ◽  
R.R.R Coelho
Keyword(s):  
Partial Characterization ◽  
Brazilian Cerrado ◽  
Streptomyces Sp

scholarly journals Optimization and partial characterization of endoglucanase produced by Streptomyces sp. B-PNG23

2013 ◽  
Vol 65 (2) ◽  
pp. 549-558 ◽  
Author(s):  
Bettache Azzeddine ◽  
Messis Abdelaziz ◽  
Copinet Estelle ◽  
Kecha Mouloud ◽  
Boucherba Nawel ◽  
...  
Keyword(s):  
Partial Characterization ◽  
Streptomyces Sp

Production and partial characterization of biosurfactant produced by Streptomyces sp. R1

2017 ◽  
Vol 40 (7) ◽  
pp. 1007-1016 ◽  
Author(s):  
Nor Syafirah Zambry ◽  
Adilah Ayoib ◽  
Nur Asshifa Md Noh ◽  
Ahmad Ramli Mohd Yahya
Keyword(s):  
Partial Characterization ◽  
Streptomyces Sp

scholarly journals Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica

2011 ◽  
Vol 42 (3) ◽  
pp. 868-877 ◽  
Author(s):  
Mihaela Cotârleţ ◽  
Teodor Gh Negoiţă ◽  
Gabriela E Bahrim ◽  
Peter Stougaard
Keyword(s):  
Partial Characterization ◽  
Streptomyces Sp ◽  
Cold Active

Chemical Constituents and Antibacterial Activity of Cissus Aralioides.

2020 ◽  
Vol 17 ◽  
Author(s):  
Balogun Olaoye Solomon ◽  
Ajayi Olukayode Solomon ◽  
Owolabi Temitayo Abidemi ◽  
Oladimeji Abdulkarbir Oladele ◽  
Liu Zhiqiang
Keyword(s):  
Plant Extract ◽  
Chemical Constituents ◽  
Ethyl Acetate Fraction ◽  
Antibacterial Activities ◽  
Sub Saharan Africa ◽  
Chromatographic Purification ◽  
Whole Plant ◽  
Sub Saharan ◽  
First Time

: Cissus aralioides is a medicinal plant used in sub-Saharan Africa for treatment of infectious diseases; however the chemical constituents of the plant have not been investigated. Thus, in this study, attempt was made at identifying predominant phytochemical constituents of the plant through chromatographic purification and silylation of the plant extract, and subsequent characterization using spectroscopic and GC-MS techniques. The minimum inhibitory concentration (MICs) for the antibacterial activities of the plant extract, chromatographic fractions and isolated compounds were also examined. Chromatographic purification of the ethyl acetate fraction from the whole plant afforded three compounds: β-sitosterol (1), stigmasterol (2) and friedelin (3). The phytosterols (1 and 2) were obtained together as a mixture. The GC-MS analysis of silylated extract indicated alcohols, fatty acids and sugars as predominant classes, with composition of 24.62, 36.90 and 26.52% respectively. Results of MICs indicated that friedelin and other chromatographic fractions had values (0.0626-1.0 mg/mL) comparable with the standard antibiotics used. Characterization of natural products from C. aralioides is being reported for the first time in this study.

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