antibacterial activities
Recently Published Documents





2022 ◽  
Vol 26 ◽  
pp. 101273
Jing Xiang ◽  
Yuzhou Li ◽  
Mingxing Ren ◽  
Ping He ◽  
Fengyi Liu ◽  

2023 ◽  
Vol 83 ◽  
S. A. Bano ◽  
S. Naz ◽  
B. Uzair ◽  
M. Hussain ◽  
M. M. Khan ◽  

Abstract Many soil microorganisms’ i.e., bacteria and fungi produce secondary metabolites called antibiotics. These are used for the treatment of some of the bacterial, fungal and protozoal diseases of humans. There is a need for isolation of a broad spectrum of antibiotics from microorganisms due to the emergence of antibiotic resistance. In the present study two antibiotic producing bacteria Klebsiella pneumoniae and Bacillus cereus were isolated from pharmaceutical and poultry feed industry of Hattar, Haripur Pakistan. Total 10 waste samples were collected from different industries (Marble, Ghee, Soap, Mineral, Steel, Poultry Feed, Pharmaceutical, Qarshi, Cosmetic and Glass). Thirty-three bacterial strains were isolated from industrial wastes of these ten different industries. Fourteen out of thirty-three bacterial strains exhibited antimicrobial activities against at least one of the test microbes considered in this study including Escherchia coli, Staphylococcus aureus and Salmonella typhi. The bacteria were isolated by standard serial dilution spread plate technique. Morphological characterization of the isolates was done by Gram staining. Nine bacterial isolates out of fourteen were initially identified as B. cereus and five as K. pneumoniae through biochemical characterization. The antibacterial activities were tested by well diffusion method. Maximum number of antibiotic producing bacteria were isolated from pharmaceutical and poultry feed industry based on the results of primary screening, the most potential isolates S9, S19, S20, S22 and S23 were selected for secondary screening. The maximum activity against E. coli and S. aureus was recorded by bacterial isolate S19 i.e zones of inhibition of 6.5mm and 9mm while S20 showed 7.5mm and 6mm zones respectively. Molecular identification was carried out on the basis of 16S rRNA sequence analysis. Finally, the isolates were identified as B. cereus accession number LC538271and K. pneumoniae accession number MT078679. Analysis of bacterial extract S20 through GC-MS indicated the presence of 8 compounds of diverse nature and structure. Present study suggests that wastes of pharmaceutical and poultry feed industry may have antibiotic producing bacteria. These bacteria could be utilized for the production of antibiotics. B. cereus and K. pneumoniae isolated from wastes of poultry feed and pharmaceutical industries have the potential to produce antibiotics and could be used to control the microbial growth.

2022 ◽  
Vol 8 ◽  
Entesar A. Hassan ◽  
Ihsan A. Shehadi ◽  
Awatef M. Elmaghraby ◽  
Hadir M. Mostafa ◽  
Salem E. Zayed ◽  

In the present study, a general approach for the synthesis of 1-(1H-indol-3-yl)-3,3-dimercaptoprop-2-en-1-one (1) and 5-(1H-indol-3-yl)-3H-1,2-dithiole-3-thione (2) was performed. They are currently used as efficient precursors for the synthesis of some new compounds bearing five- and/or six-membered heterocyclic moieties, e.g., chromenol (3, 4), 3,4-dihydroquinoline (7, 8) and thiopyran (10, 12)-based indole core. In addition, molecular docking studies were achieved, which showed that all the newly synthesized compounds are interacting with the active site region of the target enzymes, the targets UDP-N-acetylmuramatel-alanine ligase (MurC), and human lanosterol14α-demethylase, through hydrogen bonds and pi-stacked interactions. Among these docked ligand molecules, the compound (9) was found to have the minimum binding energy (−11.5 and −8.5 Kcal/mol) as compared to the standard drug ampicillin (−8.0 and −8.1 Kcal/mol) against the target enzymes UDP-N-acetylmuramatel-alanine ligase (MurC), and Human lanosterol14α-demethylase, respectively. Subsequently, all new synthesized analogues were screened for their antibacterial activities against Gram-positive (Bacillus subtilis), and Gram-negative bacteria (Escherichia coli), as well as for antifungal activities against Candida albicans and Aspergillus flavus. The obtained data suggest that the compounds exhibited good to excellent activity against bacterial and fungi strains. The compound (E)-2-(6-(1H-indole-3-carbonyl)-5-thioxotetrahydrothieno [3,2-b]furan-2(3H)-ylidene)-3-(1H-indol-3-yl)-3-oxopropanedithioic acid (9) showed a high binding affinity as well as an excellent biological activity. Therefore, it could serve as the lead for further optimization and to arrive at potential antimicrobial agent.

2022 ◽  
Ehsan Aliakbari ◽  
Reza Eghdam Zamiri ◽  
Mehri Mahdavi ◽  
Vahid Yousefi

Abstract Due to the significance of drug delivery, the design of novel progressive nanomaterials for targeting drug delivery is a significant role in pharmacotherapy, as this method is supposed to reach a more exact target. One of the most distinguished materials by researchers is the poly ionic liquid (PIL), which have been used as anticancer drugs carriers and notably improve the antitumor effect and half-life. In this work, an efficient and stable nanocarrier containing silver nanoparticle, which were well distributed throughout the ionic liquid-based copolymer network (PILP-Ag), was reported for the drug delivery with antimicrobial effect. PILP was synthesized by radical silver nanoparticles was anchored into PIL voids by in-situ reduction, which enrich the adsorption capability of drug and antimicrobial effect of the nanocarrier. The synthesized nanomaterials were characterized by various techniques such as BET, TGA, SEM, TEM, AAS and FT-IR spectroscopy. The antibacterial activities of the silver-containing PIL against both S. aureus and E. coli were studied by determination of the minimum inhibitory concentration and Minimum Bactericidal Concentration.

2022 ◽  
Shasthree Taduri ◽  
Suvarchala Vankudoth ◽  
Pavani Chirumamilla ◽  
Spoorthi Veera

The study aimed to identify bioactive compounds in <i>Muntingia calabura</i> leaf and root methanolic extracts. The Gas Chromatography and Mass Spectroscopy (GC-MS) technique were used to identify bioactive compounds. GC-MS analysis revealed 38 compounds in the leaf and 15 compounds in the root methanolic extracts of <i>M. calabura</i>. The prime potent compound found in leaf extract is 2-{3-[(E)-2-(1H-indol-3-yl)ethenyl]-1,2,4-oxadiazol-5-yl}phenol with 5.78% peak area and cholest-4-en-6-on-3-ol is found in root extracts, has the highest 63.7% peak area and another potent compound Lupeol has 7.3% peak area. The bioactive compounds identified in <i>M. calabura</i> have antibacterial activity against various bacterial strains such as gram-positive and gram-negative bacteria, which showed the efficacy of <i>in vivo</i> plant extracts. These findings validate the therapeutic potentiality of <i>M. calabura</i> leaf and root samples. Furthermore, these screened potential bioactive compounds can be used effectively for biomedical and therapeutic applications.

2022 ◽  
Vol 19 ◽  
Mohamad Hesam Shahrajabian ◽  
Wenli Sun ◽  
Qi Cheng

Abstract: Nutrition therapy on the basis of traditional medicinal plants and herbs is common in many Asian countries, especially Iran and China. Rheum species, especially rhubarbs, belong to plant medicines recognized in 2500 BC. An online search of the literature was carried out at Pubmed/Medline, Scopus, and Google scholar, covering all years until April 2021. The following key terms were used, usually in combinations: Rheum species, rhubarb, natural products, pharmaceutical benefits, anthraquinones and anthranone. After performing the literature search, the bibliographies of all articles were checked for cross-references that were not found in the search databases. Articles were selected if they reported any biological effects, ethnomedicinal uses, phytochemical compounds and botanical description of Rheum species. The most important components of rhubarb are anthraquinones, anthranone, stilbenes, tannins and butyrophenones. Anthraquinones consist of rhein, emodin, aloe-emodin and chrysophanl, and anthranone includes sennosides and rheinosides. The most important health benefits of rhubarb are antioxidant and anticancer activities, antimicrobial activity, wound healing action, hepatoprotective and anti-diabetic effects, and nephroprotective effect, as well as anti-inflammatory, analgesic and antibacterial activities. Integration of both traditional pharmaceutical science and modern medicines may promote sustainability, lead to organic life and promote the cultivation of medicinal plants.

2022 ◽  
Vol 10 (1) ◽  
pp. 145
Munirah F. Aldayel ◽  
Mayyadah A. Al Kuwayti ◽  
Nermin A. H. El Semary

Chlorella vulgaris from Al-Ahsa, KSA was proved to be an active silver and gold nanoparticle producer. Nanogold and nanosilver particles were characterized using UV-visible spectroscopy, Fourier-transform infrared spectroscopy, and scanning electronmicroscopy. Both nanoparticles were used in the antimicrobial bioassay. The two nanoparticles showed antibacterial activities, with the silver nanoparticles being the most effective. To investigate the argumentative nature of their biosynthesis (i.e., whether it is a biotic or abiotic process), we isolated total ribonucleic acid RNA as an indicator of vitality. RNA was completely absent in samples taken after one week of incubation with silver nitrate and even after one or two days. However, successful extraction was only achievable in samples taken after incubation for one and four hours with silver nitrate. Most importantly, the gel image showed recognizable shearing of the nucleic acid after 4 h as compared to the control. An assumption can be drawn that the synthesis of nanoparticles may start biotically by the action of enzyme(s) and abiotically by action of reducing entities. Nonetheless, with prolonged incubation, excessive nanoparticle accumulation can be deadly. Hence, their synthesis continues abiotically. From the RNA banding profile, we suggest that nanosilver production starts both biotically and abiotically in the first few hours of incubation and then continues abiotically. Nanosilver particles proved to have more of an antimicrobial impact than nanogold and hence are recommended for different applications as antibacterial agents.

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 416
Mostafa El Khomsi ◽  
Hamada Imtara ◽  
Mohammed Kara ◽  
Anouar Hmamou ◽  
Amine Assouguem ◽  

Anchusa italica Retz has been used for a long time in phytotherapy. The aim of the present study was to determine the antioxidant and antibacterial activities of extracts from the leaves and roots of Anchusa italica Retz. We first determined the content of phenolic compounds and flavonoids using Folin–Ciocalteu reagents and aluminum chloride (AlCl3). The antioxidant activity was determined using three methods: reducing power (FRAP), 2.2-diphenyl-1-picrylhydrazyl (DPPH), total antioxidant capacity (TAC). The antimicrobial activity was investigated against four strains of Escherichia coli, two strains of Klebsiella pneumoniae and coagulase-negative Staphylococcus, and one fungal strain of Candida albicans. The results showed that the root extract was rich in polyphenols (43.29 mg GAE/g extract), while the leave extract was rich in flavonoids (28.88 mg QE/g extract). The FRAP assay showed a strong iron reduction capacity for the root extract (IC50 of 0.11 µg/mL) in comparison to ascorbic acid (IC50 of 0.121 µg/mL). The DPPH test determined an IC50 of 0.11 µg/mL for the root extract and an IC50 of 0.14 µg/mL for the leaf extract. These values are low compared to those for ascorbic acid (IC50 of 0.16 µg/mL) and BHT (IC50 0.20 µg/mL). The TAC values of the leaf and root extracts were 0.51 and 0.98 mg AAE/g extract, respectively. In vitro, the extract showed inhibitory activity against all strains studied, with diameters of zones of inhibition in the range of 11.00–16.00 mm for the root extract and 11.67–14.33 mm for the leaf extract. The minimum inhibitory concentration was recorded for the leaf extract against E. coli (ATB:57), corresponding to 5 mg/mL. Overall, this research indicates that the extracts of Anchusa italica Retz roots and leaves exert significant antioxidant and antibacterial activities, probably because of the high content of flavonoids and polyphenols.

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 429
Ming-Der Wu ◽  
Ming-Jen Cheng ◽  
Jih-Jung Chen ◽  
Nanthaphong Khamthong ◽  
Wen-Wei Lin ◽  

Seven new compounds, including one dimer novel skeleton, chamaecyformosanin A (1); three diterpenes, chamaecyformosanins B–D (2-4); one sesquiterpene, chamaecyformosanin E (5); and two monoterpenes, chamaecyformosanins F and G (6 and 7) were isolated from the methanol extract of the bark of Chamaecyparis obtusa var. formosana. Their structures were established by the mean of spectroscopic analysis and the comparison of NMR data with those of known analogues. Their structures were elucidated on the basis of physicochemical evidence, in-depth NMR spectroscopic analysis, and high-resolution mass spectrometry. Furthermore, the isolated compounds were subjected to an evaluation of their antimicrobial activity. Metabolites 1, 3, and 4 present antibacterial activities. It is worth mentioning that the chemical composition of the bark of C. obtusa var. formosana has never been studied in the past. This is the first time the barks from C. obtusa var. formosana were studied and two new skeleton compounds, 1 and 7, were obtained.

2022 ◽  
Ebrahim Nazaripour ◽  
Farideh Mosazadeh ◽  
Seyedeh Sharifeh Rahimi ◽  
Hajar Q. Alijani ◽  
Elham Isaei ◽  

Sign in / Sign up

Export Citation Format

Share Document