scholarly journals Identification of pathogenic dematiaceous fungi and related taxa based on large subunit ribosomal DNA D1/D2 domain sequence analysis

2004 ◽  
Vol 40 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Paride Abliz ◽  
Kazutaka Fukushima ◽  
Kayoko Takizawa ◽  
Kazuko Nishimura
Keyword(s):  
Sequence Analysis ◽  
Ribosomal Dna ◽  
Large Subunit ◽  
Dematiaceous Fungi ◽  
Domain Sequence ◽  
D2 Domain

Kodamaea kakaduensisandCandida tolerans, two new ascomycetous yeast species from AustralianHibiscusflowers

1999 ◽  
Vol 45 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Marc-André Lachance ◽  
Jane M Bowles ◽  
William T Starmer ◽  
J Stuart F. Barker
Keyword(s):  
Ribosomal Dna ◽  
Yeast Species ◽  
Large Subunit ◽  
New Yeast Species ◽  
Ascomycetous Yeast ◽  
Carbon And Nitrogen ◽  
D2 Domain ◽  
New Yeast ◽  
Type Strains ◽  
Candida Mogii

Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs from others by the assimilation of trehalose, melezitose, and xylitol, and is reproductively isolated. The cells of Candida tolerans are small and a pseudomycelium is formed. The carbon and nitrogen assimilation pattern is reminiscent of that of Zygosaccharomyces rouxii but the two are not closely related. Sequences of the D1/D2 domain of large subunit ribosomal DNA confirm the membership of K. kakaduensis in the genus Kodamaea and indicate that C. tolerans belongs to the Clavispora-Metschnikowia clade, with a moderate relatedness to Candida mogii. The type strains are: K. kakaduensis, UWO(PS)98-119.2 (h+, holotype, CBS 8611) and UWO(PS)98-117.1 (h-, isotype, CBS 8612); and C. tolerans, UWO(PS)98-115.5 (CBS 8613).Key words: Kodamaea, Candida, new yeast species, ribosomal DNA, whole-cell PCR.

scholarly journals Candida andamanensis sp. nov., Candida laemsonensis sp. nov. and Candida ranongensis sp. nov., anamorphic yeast species isolated from estuarine waters in a Thai mangrove forest

2011 ◽  
Vol 61 (2) ◽  
pp. 454-461 ◽  
Author(s):  
Somjit Am-In ◽  
Savitree Limtong ◽  
Wichien Yongmanitchai ◽  
Sasitorn Jindamorakot
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
D2 Domain ◽  
Large Subunit Rrna

Five strains (RV5T, RV140, R31T, RS17 and RS28T) representing three novel anamorphic ascomycetous yeast species were isolated by membrane filtration from estuarine waters collected from a mangrove forest in Laem Son National Park, Ranong Province, Thailand, on different occasions. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and the internal transcribed spacer region and phylogenetic analysis, three strains were found to represent two novel Candida species. Two strains (RV5T and RV140) represented a single novel species, for which the name Candida laemsonensis sp. nov. is proposed. The type strain is RV5T (=BCC 35154T =NBRC 105873T =CBS 11419T). Strain R31T was assigned to a novel species that was named Candida andamanensis sp. nov. (type strain R31T =BCC 25965T =NBRC 103862T =CBS 10859T). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and phylogenetic analysis, strains RS17 and RS28T represented another novel species of Candida, for which the name Candida ranongensis sp. nov. is proposed. The type strain is RS28T (=BCC 25964T =NBRC 103861T =CBS 10861T).

A new fungicolous species of Hydropisphaera (Bionectriaceae, Hypocreales) from central China

Phytotaxa ◽  
2016 ◽  
Vol 288 (3) ◽  
pp. 279
Author(s):  
ZHAO-QING ZENG ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Sequence Analysis ◽  
Ribosomal Dna ◽  
Large Subunit ◽  
Taxonomic Position ◽  
Central China ◽  
Nuclear Ribosomal Dna ◽  
Asexual State

A new fungicolous species, Hydropisphaera spinulosa, is described and illustrated based on a collection from central China. It is characterized by non-stromatic perithecia that are solitary to gregarious, globose to subglobose, and becoming cupulate upon drying. The asci are clavate, without an apical ring, and with ascospores that are ellipsoidal to fusiform, uniseptate, and with a spinulose surface. The asexual state is acremonium-like. Comparisons are made between the new species and morphologically similar taxa. Its taxonomic position in Hydropisphaera is confirmed by sequence analysis of the large subunit of the nuclear ribosomal DNA.

Three new ascomycetous yeasts from insect-associated arboreal habitats

1999 ◽  
Vol 46 (1) ◽  
pp. 50-58
Author(s):  
C P Kurtzman
Keyword(s):  
New Species ◽  
Mating Type ◽  
Ribosomal Dna ◽  
Large Subunit ◽  
Sequence Divergence ◽  
New Yeast Species ◽  
Ascomycetous Yeast ◽  
D2 Domain ◽  
New Yeast ◽  
Ascomycetous Yeasts

A new species of Pichia and two new species of Candida are described and were determined to be genetically isolated from all other currently recognized ascomycetous yeasts from their sequence divergence in the species-variable D1/D2 domain of large subunit (26S) ribosomal DNA. The three species were primarily isolated from the frass of wood-boring insects living in pine and spruce trees. The new species and their type strains are the following: Pichia ramenticola NRRL YB-1985 (CBS 8699), mating type alpha (NRRL YB-3835, CBS 8700, mating type a), Candida piceae NRRL YB-2107 (CBS 8701), and Candida wyomingensis NRRL YB-2152 (CBS 8703). Pichia ramenticola and C. piceae assimilate methanol as a carbon source; P. ramenticola is the first known heterothallic ascomycetous yeast to utilize this compound.Key words: Pichia, Candida, new yeast species, ribosomal DNA, molecular systematics, methanol utilization.

Three new insect-associated species of the yeast genus Candida

1998 ◽  
Vol 44 (10) ◽  
pp. 965-973 ◽  
Author(s):  
C P Kurtzman ◽  
C J Robnett
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Large Subunit ◽  
Sequence Divergence ◽  
New Yeast Species ◽  
D2 Domain ◽  
Three New Species ◽  
New Yeast ◽  
Ascomycetous Yeasts ◽  
Associated Species

Three new species of Candida are described that were determined to be genetically isolated from all other currently accepted ascomycetous yeasts based on their sequence divergence in the species-variable D1/D2 domain of large subunit (26S) ribosomal DNA. One of the species was isolated from an ambrosia beetle, whereas the other two were from the frass of wood-boring beetle larvae. The new species and their type strains are the following: Candida ontarioensis NRRL YB-1246 (CBS 8502), Candida tammaniensis NRRL Y-8257 (CBS 8504), and Candida trypodendroni NRRL Y-6488 (CBS 8505).Key words: Candida, new yeast species, ribosomal DNA, molecular systematics.

scholarly journals D1/D2 Domain of Large-Subunit Ribosomal DNA for Differentiation of Orpinomyces spp.

2011 ◽  
Vol 77 (18) ◽  
pp. 6722-6725 ◽  
Author(s):  
Sumit S. Dagar ◽  
Sanjay Kumar ◽  
Priti Mudgil ◽  
Rameshwar Singh ◽  
Anil K. Puniya
Keyword(s):  
Ribosomal Dna ◽  
Restriction Site ◽  
Fragment Length Polymorphism ◽  
Large Subunit ◽  
Species Level ◽  
Additional Restriction ◽  
Lsu Rdna ◽  
Content Type ◽  
D2 Domain ◽  
Additional Restriction Site

ABSTRACTThis study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation ofOrpinomyces joyoniiandOrpinomyces intercalarisbased on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T inO. intercalariscreated an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.

scholarly journals Identification of dermatophytes by sequence analysis of the rRNA gene internal transcribed spacer regions

2008 ◽  
Vol 57 (5) ◽  
pp. 592-600 ◽  
Author(s):  
Hsin Chieh Li ◽  
Jean-Philippe Bouchara ◽  
Mark Ming-Long Hsu ◽  
Richard Barton ◽  
Shuli Su ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Trichophyton Mentagrophytes ◽  
Rrna Gene ◽  
Microsporum Canis ◽  
Its Sequencing ◽  
D2 Domain ◽  
Its Sequence Analysis ◽  
Large Subunit Rrna

Identification of dermatophytes using the traditional method is sometimes problematic because of atypical microscopic or macroscopic morphology. The aim of this study was to evaluate the feasibility of using sequencing of the ribosomal internal transcribed spacer (ITS)1 and ITS2 regions for identification of 17 dermatophyte species. The ITS regions of 188 strains (62 reference strains and 126 clinical isolates) were amplified by PCR and sequenced. Species identification was made by sequence comparison with an in-house database comprising ITS sequences of type or neotype strains or by blast searches for homologous sequences in public databases. Strains producing discrepant results between conventional methods and ITS sequence analysis were analysed further by sequencing the D1–D2 domain of the large-subunit rRNA gene for species clarification. The identification rates by ITS1 and ITS2 sequencing were higher than 97 %. Based on reference sequences of type or neotype strains, it was noted that most strains of Trichophyton mentagrophytes were misidentifications of Trichophyton interdigitale. In addition, barcode sequences were present in species of the Microsporum canis complex and Trichophyton rubrum complex. These barcode sequences are useful for species delineation when the results of ITS sequencing are ambiguous. In conclusion, ITS sequencing provides a very accurate and useful method for the identification of dermatophytes.

Sign in / Sign up

Export Citation Format

Share Document