Three new ascomycetous yeasts from insect-associated arboreal habitats

1999 ◽  
Vol 46 (1) ◽  
pp. 50-58
Author(s):  
C P Kurtzman
Keyword(s):  
New Species ◽  
Mating Type ◽  
Ribosomal Dna ◽  
Large Subunit ◽  
Sequence Divergence ◽  
New Yeast Species ◽  
Ascomycetous Yeast ◽  
D2 Domain ◽  
New Yeast ◽  
Ascomycetous Yeasts

A new species of Pichia and two new species of Candida are described and were determined to be genetically isolated from all other currently recognized ascomycetous yeasts from their sequence divergence in the species-variable D1/D2 domain of large subunit (26S) ribosomal DNA. The three species were primarily isolated from the frass of wood-boring insects living in pine and spruce trees. The new species and their type strains are the following: Pichia ramenticola NRRL YB-1985 (CBS 8699), mating type alpha (NRRL YB-3835, CBS 8700, mating type a), Candida piceae NRRL YB-2107 (CBS 8701), and Candida wyomingensis NRRL YB-2152 (CBS 8703). Pichia ramenticola and C. piceae assimilate methanol as a carbon source; P. ramenticola is the first known heterothallic ascomycetous yeast to utilize this compound.Key words: Pichia, Candida, new yeast species, ribosomal DNA, molecular systematics, methanol utilization.

Three new insect-associated species of the yeast genus Candida

1998 ◽  
Vol 44 (10) ◽  
pp. 965-973 ◽  
Author(s):  
C P Kurtzman ◽  
C J Robnett
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Large Subunit ◽  
Sequence Divergence ◽  
New Yeast Species ◽  
D2 Domain ◽  
Three New Species ◽  
New Yeast ◽  
Ascomycetous Yeasts ◽  
Associated Species

Three new species of Candida are described that were determined to be genetically isolated from all other currently accepted ascomycetous yeasts based on their sequence divergence in the species-variable D1/D2 domain of large subunit (26S) ribosomal DNA. One of the species was isolated from an ambrosia beetle, whereas the other two were from the frass of wood-boring beetle larvae. The new species and their type strains are the following: Candida ontarioensis NRRL YB-1246 (CBS 8502), Candida tammaniensis NRRL Y-8257 (CBS 8504), and Candida trypodendroni NRRL Y-6488 (CBS 8505).Key words: Candida, new yeast species, ribosomal DNA, molecular systematics.

Kodamaea kakaduensisandCandida tolerans, two new ascomycetous yeast species from AustralianHibiscusflowers

1999 ◽  
Vol 45 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Marc-André Lachance ◽  
Jane M Bowles ◽  
William T Starmer ◽  
J Stuart F. Barker
Keyword(s):  
Ribosomal Dna ◽  
Yeast Species ◽  
Large Subunit ◽  
New Yeast Species ◽  
Ascomycetous Yeast ◽  
Carbon And Nitrogen ◽  
D2 Domain ◽  
New Yeast ◽  
Type Strains ◽  
Candida Mogii

Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs from others by the assimilation of trehalose, melezitose, and xylitol, and is reproductively isolated. The cells of Candida tolerans are small and a pseudomycelium is formed. The carbon and nitrogen assimilation pattern is reminiscent of that of Zygosaccharomyces rouxii but the two are not closely related. Sequences of the D1/D2 domain of large subunit ribosomal DNA confirm the membership of K. kakaduensis in the genus Kodamaea and indicate that C. tolerans belongs to the Clavispora-Metschnikowia clade, with a moderate relatedness to Candida mogii. The type strains are: K. kakaduensis, UWO(PS)98-119.2 (h+, holotype, CBS 8611) and UWO(PS)98-117.1 (h-, isotype, CBS 8612); and C. tolerans, UWO(PS)98-115.5 (CBS 8613).Key words: Kodamaea, Candida, new yeast species, ribosomal DNA, whole-cell PCR.

scholarly journals Description of four Apiotrichum and two Cutaneotrichosporon species isolated from guano samples from bat-inhabited caves in Japan

2020 ◽  
Vol 70 (8) ◽  
pp. 4458-4469
Author(s):  
Masako Takashima ◽  
Sanae Kurakado ◽  
Otomi Cho ◽  
Ken Kikuchi ◽  
Junta Sugiyama ◽  
...  
Keyword(s):  
New Species ◽  
Yeast Species ◽  
Large Subunit ◽  
Biochemical Properties ◽  
Rrna Genes ◽  
New Yeast Species ◽  
Rrna Gene ◽  
D2 Domain ◽  
Lsu Rrna ◽  
New Yeast

Four new yeast species belonging to the genus Apiotrichum and two new yeast species belonging to Cutaneotrichosporon are described for strains isolated from guano samples from bat-inhabited caves in Japan. In 2005, we reported these isolates as Trichosporon species based on sequence analyses of the D1/D2 domain of large subunit (LSU) rRNA genes according to available basidiomycetous yeast classification criteria; however, to date, they have not been officially published as new species with descriptions. Their phylogenetic positions have been reanalysed based on comparison of internal transcribed spacer (ITS) region sequences (including the 5.8S rRNA gene) and the D1/D2 domain of the LSU rRNA gene with those of known species; we confirmed clear separation from previously described species. Physiological and biochemical properties of the isolates also suggest their distinctiveness. Therefore, we describe Apiotrichum akiyoshidainum (holotype JCM 12595T), Apiotrichum chiropterorum (JCM 12594T), Apiotrichum coprophilum (JCM 12596T), Apiotrichum otae (JCM 12593T), Cutaneotrichosporon cavernicola (JCM 12590T) and Cutaneotrichosporon middelhovenii (JCM 12592T) as new species. C. cavernicola showed particularly distinctive morphology including large inflated anomalous cells on the hyphae and germination from the cells, although clear clamp connections on the hyphae were not confirmed. Further study is needed to elucidate the morph of this species.

scholarly journals Candida heliconiae sp. nov., Candida picinguabensis sp. nov. and Candida saopaulonensis sp. nov., three ascomycetous yeasts from Heliconia velloziana (Heliconiaceae)

2006 ◽  
Vol 56 (5) ◽  
pp. 1147-1151 ◽  
Author(s):  
Carla C. C. Ruivo ◽  
Marc-André Lachance ◽  
Carlos A. Rosa ◽  
Maurício Bacci ◽  
Fernando C. Pagnocca
Keyword(s):  
Forest Ecosystem ◽  
Yeast Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Eastern Brazil ◽  
D2 Domain ◽  
Large Subunit Rdna ◽  
Ascomycetous Yeasts ◽  
Year 2000 ◽  
Type Strains

Strains belonging to three novel yeast species, Candida heliconiae (four isolates), Candida picinguabensis (three isolates) and Candida saopaulonensis (two isolates), were recovered in the year 2000 from water of flower bracts of Heliconia velloziana L. Emigd. (Heliconiaceae) found in a forest ecosystem site in an Atlantic rainforest of south-eastern Brazil. C. picinguabensis and C. saopaulonensis were nearly identical in morphology and physiology, but sequence divergence in the D1/D2 domain of the large-subunit rDNA indicated that they should be regarded as different species. They belong to the Metschnikowiaceae clade. C. heliconiae had affinities to Pichia mexicana and related species, but was genetically isolated from all currently accepted species in that group. The type strains are C. heliconiae UNESP 00-91C1T (=CBS 10000T=NRRL Y-27813T), C. picinguabensis UNESP 00-89T (=CBS 9999T=NRRL Y-27814T) and C. saopaulonensis UNESP 00-99T (=CBS 10001T=NRRL Y-27815T).

scholarly journals Papiliotrema maritimi f.a. sp. nov., a new tremellaceous yeast species associated to macrophytes in a Marshland of South Brazil

2020 ◽  
Author(s):  
Mauricio Ramírez-Castrillón ◽  
Fernanda Fraga Gomes ◽  
Andrea Formoso de Souza ◽  
Belize Rodrigues Leite ◽  
Danielle Machado Pagani ◽  
...  
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Its Region ◽  
New Yeast Species ◽  
Rrna Gene ◽  
Phylogenetic Species Concept ◽  
D2 Domain ◽  
Lsu Rrna Gene ◽  
New Yeast ◽  
South Brazil

AbstractOne new yeast species, Papiliotrema maritimi sp. nov., is being proposed to be suitable into the Rhynchogastremataceae family, belonging to the Tremellales clade. This new species is related to six others from the Papiliotrema genus: P. taeanensis, P. siamense, P. perniciosus, P. nemorosus, P. bandonii, P. japonica and P. fuscus. The novel species is proposed based on the phylogenetic species concept through analysis of the D1/D2 region, part of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. A total of 11 strains of Papiliotrema maritimi sp. nov. were obtained from macrophytes leaves collected in south Brazil. Papiliotrema maritimi sp. nov. differs by 12, 15, 25, 25, 25 and 29 substitutions in the D1/D2 domain from the related species P. fuscus, P. japonica, P. siamense, P. nemorosus, P. bandonii, and P. perniciosus, respectively. Concerning the ITS region, there are 11 substitutions and 52 or more substitutions when compared to P. teanensis and its closest relatives. The type strain of Papiliotrema maritimi sp. nov. is UFMG-CM-Y6048. The MycoBank number for Papiliotrema maritimi sp. nov. is MB 835603.

Candida ipomoeae, a new yeast species related to large-spored Metschnikowia species

1998 ◽  
Vol 44 (8) ◽  
pp. 718-722 ◽  
Author(s):  
Marc-André Lachance ◽  
Carlos A Rosa ◽  
William T Starmer ◽  
Jane M Bowles
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Single Species ◽  
Morning Glory ◽  
New Yeast Species ◽  
Nutritional Profile ◽  
D2 Domain ◽  
Sap Beetles ◽  
Agar Media ◽  
New Yeast

Numerous strains of an unusual asexual yeast species were isolated from flowers of morning glory (Ipomoea spp., Convolvulaceae) and associated drosophilids and sap beetles of the genus Conotelus sampled in Hawaii and in Brazil. The nutritional profile of this yeast is similar to those of Metschnikowia hawaiiensis and Metschnikowia continentalis, which share the same habitats. The cells are large, hydrophobic, and tend to remain attached after budding, causing the colonies on agar media to have a convoluted appearance, reminiscent of popcorn. The sequences of the D1/D2 domain of large subunit rDNAs of strains from three different localities confirmed that a single species is involved, and that it is related to large-spored Metschnikowia species. The type strain is UWO(PS)91-672.1 (CBS 8466).Key words: Candida ipomoeae, yeast, new species, rDNA.

scholarly journals BIODIVERSITY OF YEASTS ISOLATED FROM CON DAO ISLAND – BA RIA VUNG TAU

2018 ◽  
Vol 55 (1A) ◽  
pp. 99
Author(s):  
Dao Thi Luong
Keyword(s):  
New Species ◽  
Yeast Species ◽  
Candida Rugosa ◽  
New Yeast Species ◽  
Yeast Diversity ◽  
26S Rdna ◽  
Diverse Species ◽  
Yeast Strains ◽  
D2 Domain ◽  
New Yeast

During the study on yeast diversity from natural samples in Viet Nam, we have collected 38 samples of flowers, fruits, leaves, litter and soils in Con Dao Island and isolated 54 yeast strains. They were identified based on morphology observation and sequences of D1/D2 domain of 26S rDNA. The results showed that they belonged to 13 genera, 28 species, of which 15 strains were suspected as 13 new species. Thirty-nine strains were identified as Ascomycetes belonging 7 genera, 11 species, and the fifteen remaining strains belonged to Basidomycetes, 6 genera and 13 species. The results also showed that four Ascomycetous and nine Basidomycetous isolated were suspected to be new species. The results also showed that Candida was the genus with highest number isolates- 26 strains, and half of these were Candida rugosa. The most diverse species in this study was found in Cryptococcus genus with 7 species from 8 isolates only, and all of them were suspected to be new species. This study also proved that the samples had differences in yeast diversity and species composition among them. Therefore, Con Dao Island is multi potential to discover and publish new yeast species in Vietnam. 

scholarly journals Partial Sequences of Large Subunit Ribosomal DNA of a New Yeast Species,Trichosporon domesticumand Related Species

1997 ◽  
Vol 41 (7) ◽  
pp. 571-573 ◽  
Author(s):  
Takashi Sugita ◽  
Koichi Makimura ◽  
Akemi Nishikawa ◽  
Katsuhisa Uchida ◽  
Hideyo Yamaguchi ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Related Species ◽  
Yeast Species ◽  
Large Subunit ◽  
New Yeast Species ◽  
New Yeast

scholarly journals Candida gosingica sp. nov., an anamorphic ascomycetous yeast closely related to Scheffersomyces spartinae

2011 ◽  
Vol 61 (3) ◽  
pp. 690-694 ◽  
Author(s):  
Chin-Feng Chang ◽  
Cheng-Hsu Yao ◽  
Shuh-Sen Young ◽  
Savitree Limtong ◽  
Rungluk Kaewwichian ◽  
...  
Keyword(s):  
Large Subunit ◽  
Sequence Divergence ◽  
Small Subunit ◽  
Ssu Rdna ◽  
Lsu Rdna ◽  
The Novel ◽  
Ascomycetous Yeast ◽  
Centraalbureau Voor ◽  
5.8S Rdna ◽  
D2 Domain

During surveys on yeast diversity in forest soils from Taiwan and Thailand, ten yeast strains isolated from different samples were found to have similar molecular and physiological characteristics. Sequence analysis of small subunit (SSU) rDNA, the D1/D2 domain of large subunit (LSU) rDNA and internal transcribed spacer (ITS)-5.8S rDNA demonstrated that these strains were closely related to Scheffersomyces spartinae. The novel strains could be differentiated from S. spartinae by a 0.9 % sequence divergence (5 substitutions, 0 gaps) in the D1/D2 domain of LSU rDNA, a 1.5 % divergence (8 substitutions, 0 gaps) in the ITS-5.8S rDNA and a 0.7 % divergence (12 substitutions, 2 gaps) in the SSU rDNA. The novel strains also showed specific patterns of electrophoretic karyotypes that differed from that of S. spartinae. Therefore, a novel yeast species, Candida gosingica sp. nov., is proposed to accommodate these strains. The type strain SJ7S11T (=BCRC 23194T=CBS 11433T) was assigned and deposited in the Bioresource Collection and Research Center (BCRC), Food Industry Development and Research Institute, Hsinchu, Taiwan, and Centraalbureau voor Schimmelcultures (CBS), Utrecht, The Netherlands.

scholarly journals Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil

2010 ◽  
Vol 60 (1) ◽  
pp. 244-248 ◽  
Author(s):  
Melissa Fontes Landell ◽  
Raisa Billodre ◽  
Jesus P. Ramos ◽  
Orílio Leoncini ◽  
Marilene H. Vainstein ◽  
...  
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Sequence Divergence ◽  
Rrna Gene ◽  
The Novel ◽  
D2 Domain ◽  
Its Gene ◽  
Ascomycetous Yeasts ◽  
Positive Results

Two novel yeast species, Candida aechmeae sp. nov. and Candida vrieseae sp. nov., were isolated from bromeliads in Itapuã Park, Rio Grande do Sul, Brazil. These species are genetically isolated from all other currently recognized ascomycetous yeasts based on their sequence divergence in the D1/D2 domain of the LSU rRNA gene. C. aechmeae sp. nov. is phylogenetically close to Candida ubatubensis, a species also isolated from bromeliads in Brazil, but the novel species can be differentiated on the basis of differences in the D1/D2 domain and positive results for the assimilation of l-arabinose, raffinose, inulin and citrate. Candida vrieseae sp. nov. is phylogenetically placed in a clade near Candida membranifaciens that is composed of several species associated with insects, but the novel species can be differentiated from them by the D1/D2 and ITS gene sequences, positive results for the assimilation of nitrite and a negative result for the assimilation of ethylamine. The type strain for Candida aechmeae sp. nov. is BI153T (=CBS 10831T=NRRL Y-48456T) and the type strain for C. vrieseae sp. nov. is BI146T (=CBS 10829T=NRRL Y-48461T).

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