The use of phospholipid and neutral lipid fatty acids to estimate biomass of arbuscular mycorrhizal fungi in soil

1995 ◽  
Vol 99 (5) ◽  
pp. 623-629 ◽  
Author(s):  
Pål Axel Olsson ◽  
Erland Bååth ◽  
Iver Jakobsen ◽  
Bengt Söderström
Keyword(s):  
Fatty Acids ◽  
Arbuscular Mycorrhizal Fungi ◽  
Neutral Lipid ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal

scholarly journals Symbiotic association between golden berry (Physalis peruviana) and arbuscular mycorrhizal fungi in heavy metal-contaminated soil

2017 ◽  
Vol 57 (2) ◽  
pp. 173-184 ◽  
Author(s):  
Marieta Hristozkova ◽  
Maria Geneva ◽  
Ira Stancheva ◽  
Ivan Iliev ◽  
Concepción Azcón-Aguilar
Keyword(s):  
Fatty Acids ◽  
Heavy Metal ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Protein Accumulation ◽  
Symbiotic Association ◽  
Tropical Fruit ◽  
Physalis Peruviana ◽  
Mycorrhizal Association

AbstractPhysalis peruvianais one of the most promising tropical fruit plants because of its rapid growth, high yield, and nutritional quality. This study was designed to investigate plant development under heavy metal contamination (Cd, Pb) and responsiveness to arbuscular mycorrhizal fungi (AMF) colonization byRhizophagus clarumandClaroideoglomus claroideum. The antioxidant capacity, total lipid content and fatty acid profile in fruits, accumulation of Cd and Pb in different plant parts, plant dry biomass, and mycorrhizal colonization were determined. As a result of inoculation, a considerable reduction in Cd and Pb in the fruits was observed, compared with non-inoculated plants. The fruit number and dry weight increased in plants associated withC. claroideum.These plants also showed higher acid phosphatase activity, root protein accumulation and glomalin production. The type of antioxidant defense was AMF strain-dependent. Antioxidant activity and H2O2neutralization were enzymatic rather than non-enzymatic processes in the fruits ofC. claroideumplants compared with those forming an association withR. clarum. Mycorrhizal establishment changed the composition and concentration of fruits’ fatty acids. The ratio of unsaturated fatty acids was increased. With respect to the accumulation of bioactive compounds in golden berry the present findings are important for obtaining the optimum benefits of mycorrhizal association under unfavorable conditions.

scholarly journals Fatty acids in arbuscular mycorrhizal fungi are synthesized by the host plant

Science ◽  
2017 ◽  
Vol 356 (6343) ◽  
pp. 1175-1178 ◽  
Author(s):  
Leonie H. Luginbuehl ◽  
Guillaume N. Menard ◽  
Smita Kurup ◽  
Harrie Van Erp ◽  
Guru V. Radhakrishnan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Arbuscular Mycorrhizal Fungi ◽  
Host Plant ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal

scholarly journals 13C Incorporation into Signature Fatty Acids as an Assay for Carbon Allocation in Arbuscular Mycorrhiza

2005 ◽  
Vol 71 (5) ◽  
pp. 2592-2599 ◽  
Author(s):  
Pål Axel Olsson ◽  
Ingrid M. van Aarle ◽  
Mayra E. Gavito ◽  
Per Bengtson ◽  
Göran Bengtsson
Keyword(s):  
Fatty Acid ◽  
Arbuscular Mycorrhizal Fungi ◽  
Neutral Lipid ◽  
Mycorrhizal Fungi ◽  
Carbon Allocation ◽  
Arbuscular Mycorrhizal ◽  
Extraradical Mycelium ◽  
Lipid Fatty Acid ◽  
Neutral Lipid Fatty Acid ◽  
Carbon Transfer

ABSTRACT The ubiquitous arbuscular mycorrhizal fungi consume significant amounts of plant assimilated C, but this C flow has been difficult to quantify. The neutral lipid fatty acid 16:1ω5 is a quantitative signature for most arbuscular mycorrhizal fungi in roots and soil. We measured carbon transfer from four plant species to the arbuscular mycorrhizal fungus Glomus intraradices by estimating 13C enrichment of 16:1ω5 and compared it with 13C enrichment of total root and mycelial C. Carbon allocation to mycelia was detected within 1 day in monoxenic arbuscular mycorrhizal root cultures labeled with [13C]glucose. The 13C enrichment of neutral lipid fatty acid 16:1ω5 extracted from roots increased from 0.14% 1 day after labeling to 2.2% 7 days after labeling. The colonized roots usually were more enriched for 13C in the arbuscular mycorrhizal fungal neutral lipid fatty acid 16:1ω5 than for the root specific neutral lipid fatty acid 18:2ω6,9. We labeled plant assimilates by using 13CO2 in whole-plant experiments. The extraradical mycelium often was more enriched for 13C than was the intraradical mycelium, suggesting rapid translocation of carbon to and more active growth by the extraradical mycelium. Since there was a good correlation between 13C enrichment in neutral lipid fatty acid 16:1ω5 and total 13C in extraradical mycelia in different systems (r 2 = 0.94), we propose that the total amount of labeled C in intraradical and extraradical mycelium can be calculated from the 13C enrichment of 16:1ω5. The method described enables evaluation of C flow from plants to arbuscular mycorrhizal fungi to be made without extraction, purification and identification of fungal mycelia.

scholarly journals Fatty Acid Composition and Eco-Agronomical Traits of Lallemantia Species Modulated Upon Exposed To Arbuscular Mycorrhizal Fungi and Nano Iron Chelate Fertilizers Under Water Deficit Conditions

2021 ◽  
Author(s):  
Arezoo Paravar ◽  
Saeideh Maleki Farahani ◽  
Ali Reza Rezazadeh
Keyword(s):  
Fatty Acids ◽  
Arbuscular Mycorrhizal Fungi ◽  
Water Deficit ◽  
Mycorrhizal Fungi ◽  
Seed Oil ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal ◽  
Water Deficit Stress ◽  
Iron Chelate ◽  
Amf Inoculation

Abstract Background: Application nano iron chelate and AMF fertilizer can increase plants' tolerance against water deficit stress. The main objectives of the current study were to investigate the effect of arbuscular mycorrhizal fungi (AMF) and nano iron chelate fertilizer under drought stress on grain yield, leaf chlorophyll contents, root colonization, oil percentage, and fatty acids profile of Lallemantia species. The experiment was carried out as a factorial based on a complete randomized block design consisting of three factors of irrigation levels of 90 (I90), 60 (I60), and 30% (I30) depletion of available soil water (ASW)), fertilizer levels of control (no fertilizer), AMF inoculation, and nano iron chelate, and plant species of Lallemantia (L. iberica and L. royleana) at the Research Farm of College of Agriculture, Shahed University, Tehran, Iran, in 2018/2019. Results: The results showed that increasing water deficit stress significantly decreased the above traits while applying nano iron and AMF fertilizers significantly increased them across water treatments. AMF fertilizer inoculation significantly improved both species yield. Higher root colonization by AMF inoculation enhanced seed oil and fatty acids (palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, and Eicosenoic acid). In contrast, applying nano iron chelate by increasing chlorophyll content in any irrigation regime could enhance seed oil and some fatty acids such as palmitoleic acid. Conclusions: Water deficit stress and application of fertilizers had different effects on both species. L. iberica, compared to L. royleana, had the most tolerance to water deficit stress and the highest dependence on AMF inoculation. Overall, these results demonstrated that the application of AMF could improve major features of Lallemantia species under deficit irrigation conditions, especially at the I60 irrigation level.

Stimulation of asymbiotic sporulation in arbuscular mycorrhizal fungi by fatty acids

2019 ◽  
Vol 4 (10) ◽  
pp. 1654-1660 ◽  
Author(s):  
Hiromu Kameoka ◽  
Ippo Tsutsui ◽  
Katsuharu Saito ◽  
Yusuke Kikuchi ◽  
Yoshihiro Handa ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Stimulation Of

scholarly journals Dependence of Arbuscular-Mycorrhizal Fungi on Their Plant Host for Palmitic Acid Synthesis

2005 ◽  
Vol 71 (9) ◽  
pp. 5341-5347 ◽  
Author(s):  
Martin Trépanier ◽  
Guillaume Bécard ◽  
Peter Moutoglis ◽  
Claude Willemot ◽  
Serge Gagné ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Arbuscular Mycorrhizal Fungi ◽  
Fatty Acid Synthesis ◽  
Mycorrhizal Fungi ◽  
Fatty Acid Synthase ◽  
Arbuscular Mycorrhizal ◽  
Acid Synthesis ◽  
Fungal Mycelium ◽  
Plant Host

ABSTRACT Lipids are the major form of carbon storage in arbuscular-mycorrhizal fungi. We studied fatty acid synthesis by Glomus intraradices and Gigaspora rosea. [14C]Acetate and [14C]sucrose were incorporated into a synthetic culture medium to test fatty acid synthetic ability in germinating spores (G. intraradices and G. rosea), mycorrhized carrot roots, and extraradical fungal mycelium (G. intraradices). Germinating spores and extraradical hyphae could not synthesize 16-carbon fatty acids but could elongate and desaturate fatty acids already present. The growth stimulation of germinating spores by root exudates did not stimulate fatty acid synthesis. 16-Carbon fatty acids (16:0 and 16:1) were synthesized only by the fungi in the mycorrhized roots. Our data strongly suggest that the fatty acid synthase activity of arbuscular-mycorrhizal fungi is expressed exclusively in the intraradical mycelium and indicate that fatty acid metabolism may play a major role in the obligate biotrophism of arbuscular-mycorrhizal fungi.

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