Modeling data from titration, amide H/D exchange, and mass spectrometry to obtain protein-ligand binding constants

2004 ◽  
Author(s):  
M Zhu
Keyword(s):  
Mass Spectrometry ◽  
Ligand Binding ◽  
Binding Constants ◽  
Modeling Data

scholarly journals Unpicking allosteric mechanisms of homo-oligomeric proteins by determining their successive ligand binding constants

2018 ◽  
Vol 373 (1749) ◽  
pp. 20170176 ◽  
Author(s):  
Ranit Gruber ◽  
Amnon Horovitz
Keyword(s):  
Mass Spectrometry ◽  
Ligand Binding ◽  
Single Molecule ◽  
Binding Constants ◽  
Molecular Machines ◽  
Native Mass Spectrometry ◽  
Oligomeric Proteins ◽  
Allosteric Mechanisms ◽  
The Relationship

Advances in native mass spectrometry and single-molecule techniques have made it possible in recent years to determine the values of successive ligand binding constants for large multi-subunit proteins. Given these values, it is possible to distinguish between different allosteric mechanisms and, thus, obtain insights into how various bio-molecular machines work. Here, we describe for ring-shaped homo-oligomers, in particular, how the relationship between the values of successive ligand binding constants is diagnostic for concerted, sequential and probabilistic allosteric mechanisms. This article is part of a discussion meeting issue ‘Allostery and molecular machines’.

Determination of Protein–Ligand Binding Constants of a Cooperatively Regulated Tetrameric Enzyme Using Electrospray Mass Spectrometry

2013 ◽  
Vol 9 (1) ◽  
pp. 218-226 ◽  
Author(s):  
Dragana Cubrilovic ◽  
Wolfgang Haap ◽  
Konstantin Barylyuk ◽  
Armin Ruf ◽  
Martin Badertscher ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ligand Binding ◽  
Electrospray Mass Spectrometry ◽  
Binding Constants
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